Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NF674_RS22150 Genome accession   NZ_CP099610
Coordinates   5008420..5009637 (+) Length   405 a.a.
NCBI ID   WP_252875096.1    Uniprot ID   -
Organism   Pseudomonas moraviensis strain OTU5VILLAA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5003420..5014637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF674_RS22130 (NF674_22130) - 5004833..5005132 (+) 300 WP_064364128.1 DUF2845 domain-containing protein -
  NF674_RS22135 (NF674_22135) - 5005400..5005753 (-) 354 WP_042610476.1 BON domain-containing protein -
  NF674_RS22140 (NF674_22140) - 5006053..5006490 (-) 438 WP_064364126.1 pilin -
  NF674_RS22145 (NF674_22145) pilB 5006717..5008417 (+) 1701 WP_042610478.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NF674_RS22150 (NF674_22150) pilC 5008420..5009637 (+) 1218 WP_252875096.1 type II secretion system F family protein Machinery gene
  NF674_RS22155 (NF674_22155) pilD 5009639..5010511 (+) 873 WP_252875097.1 A24 family peptidase Machinery gene
  NF674_RS22160 (NF674_22160) coaE 5010508..5011131 (+) 624 WP_252875098.1 dephospho-CoA kinase -
  NF674_RS22165 (NF674_22165) yacG 5011128..5011328 (+) 201 WP_042610482.1 DNA gyrase inhibitor YacG -
  NF674_RS22170 (NF674_22170) - 5011378..5012067 (-) 690 WP_252875099.1 energy-coupling factor ABC transporter permease -
  NF674_RS22175 (NF674_22175) - 5012257..5012883 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -
  NF674_RS22180 (NF674_22180) - 5012880..5013407 (+) 528 WP_065615663.1 MOSC domain-containing protein -
  NF674_RS22185 (NF674_22185) - 5013459..5013632 (+) 174 WP_007909875.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44089.75 Da        Isoelectric Point: 10.1214

>NTDB_id=701110 NF674_RS22150 WP_252875096.1 5008420..5009637(+) (pilC) [Pseudomonas moraviensis strain OTU5VILLAA1]
MAVKAAKVSIYAWEGTDRKGSKVSGELSGQNPALIKAQLRKQGINPGKVRKKSASLLSFGKRIKPQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFATSLRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
EALKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPGFTLMIISLSEFMQQWWWAILFALVAAFFGTR
HALKTSQALRDRRDAWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRVRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDDMLDKVASFYEAEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=701110 NF674_RS22150 WP_252875096.1 5008420..5009637(+) (pilC) [Pseudomonas moraviensis strain OTU5VILLAA1]
ATGGCGGTCAAGGCAGCAAAAGTCAGCATTTACGCCTGGGAAGGCACGGACCGCAAAGGCAGCAAAGTCAGCGGCGAGTT
GAGTGGGCAGAATCCCGCACTGATCAAGGCGCAGCTGCGCAAACAGGGGATCAACCCGGGCAAGGTGCGCAAGAAATCCG
CCTCCCTGCTGAGCTTCGGCAAACGCATCAAGCCGCAGGACATCGCCTTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGCGTGCCGCTGTTGCAGTCCTTCGACATCATCGGCGAGGGCTTCGAAAACCCGGCCATGCGCAAACTCGTCGACGA
GGTCAAACAGGAGGTCGCCGCCGGTAACAGCTTCGCCACGTCATTGCGCAAAAAACCGCAATACTTCGACGAGTTGTACT
GCAATCTGGTCGACGCTGGCGAACAGTCCGGCGCCCTCGATACCTTGCTCGAACGGGTTGCGACCTATAAGGAAAAAAGC
GAAGCGCTCAAGGCCAAGATCAAGAAGGCGATGACTTACCCTACTGCCGTGGTGCTGGTGGCGGCAGTGGTGACCGGTAT
TCTGCTGGTCAAAGTGGTGCCGCAGTTCCAGTCGGTGTTTTCCGGGTTCGGCGCCGAATTGCCGGGCTTCACTCTGATGA
TCATCAGCCTGTCGGAGTTCATGCAGCAGTGGTGGTGGGCGATTCTCTTTGCCCTGGTCGCGGCGTTTTTCGGCACTCGC
CACGCGCTGAAGACTTCGCAGGCCCTGCGCGACCGTCGCGATGCCTGGCTGCTGAAGCTGCCGCTGGTGGGCACCTTGAT
GTACAAGTCCGCCGTTGCACGTTTTGCCCGCACCTTGTCGACTACATTCGCAGCCGGTGTGCCGTTGGTGGAAGCGCTCG
ATTCGGTCGCCGGCGCCACCGGCAACGTGGTGTTCAAGCGTGCGGTGCTGCGGGTCCGGCAGGACGTGTCGACCGGCATG
CAGCTGAATTTCTCGATGCGCAGCACCGGTGTATTCCCCAACATGGCGGTACAAATGACCGCCATCGGCGAGGAGTCCGG
GGCACTGGATGACATGCTCGACAAGGTTGCCAGTTTCTATGAGGCCGAAGTCGATAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGGGTCATCGTCGGTGGACTGGTGGTGGCCATGTACCTGCCGATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.321

100

0.743
  pilC Acinetobacter baylyi ADP1

58.333

100

0.588
  pilC Acinetobacter baumannii D1279779

58.333

100

0.588
  pilC Legionella pneumophila strain ERS1305867

53

98.765

0.523
  pilC Vibrio cholerae strain A1552

44.444

97.778

0.435
  pilG Neisseria gonorrhoeae MS11

43.176

99.506

0.43
  pilG Neisseria meningitidis 44/76-A

42.432

99.506

0.422
  pilC Vibrio campbellii strain DS40M4

41.379

100

0.415
  pilC Thermus thermophilus HB27

39.098

98.519

0.385