Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NF674_RS22145 Genome accession   NZ_CP099610
Coordinates   5006717..5008417 (+) Length   566 a.a.
NCBI ID   WP_042610478.1    Uniprot ID   -
Organism   Pseudomonas moraviensis strain OTU5VILLAA1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5001717..5013417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF674_RS22125 (NF674_22125) - 5001885..5004836 (+) 2952 WP_252875095.1 DUF748 domain-containing protein -
  NF674_RS22130 (NF674_22130) - 5004833..5005132 (+) 300 WP_064364128.1 DUF2845 domain-containing protein -
  NF674_RS22135 (NF674_22135) - 5005400..5005753 (-) 354 WP_042610476.1 BON domain-containing protein -
  NF674_RS22140 (NF674_22140) - 5006053..5006490 (-) 438 WP_064364126.1 pilin -
  NF674_RS22145 (NF674_22145) pilB 5006717..5008417 (+) 1701 WP_042610478.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NF674_RS22150 (NF674_22150) pilC 5008420..5009637 (+) 1218 WP_252875096.1 type II secretion system F family protein Machinery gene
  NF674_RS22155 (NF674_22155) pilD 5009639..5010511 (+) 873 WP_252875097.1 A24 family peptidase Machinery gene
  NF674_RS22160 (NF674_22160) coaE 5010508..5011131 (+) 624 WP_252875098.1 dephospho-CoA kinase -
  NF674_RS22165 (NF674_22165) yacG 5011128..5011328 (+) 201 WP_042610482.1 DNA gyrase inhibitor YacG -
  NF674_RS22170 (NF674_22170) - 5011378..5012067 (-) 690 WP_252875099.1 energy-coupling factor ABC transporter permease -
  NF674_RS22175 (NF674_22175) - 5012257..5012883 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -
  NF674_RS22180 (NF674_22180) - 5012880..5013407 (+) 528 WP_065615663.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62597.63 Da        Isoelectric Point: 6.0450

>NTDB_id=701109 NF674_RS22145 WP_042610478.1 5006717..5008417(+) (pilB) [Pseudomonas moraviensis strain OTU5VILLAA1]
MNDIALSGLAKQLVQAELLTETSARQSWAQAQRNRVSLVNYLVHNKLVNSRQIAEIASEHFGMALLDLNCLDKETQPKGL
VSEKLVRQHCALPLWRRGNKLFVGLSDPSNQQAISDIQFSTGLSTEAILVEDDKLSDAIDKFFDSHASGLEEMADVDLDG
LDVESIDDNKQDVIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRMRTDGMLREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEAEQKALYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCAHCKRPLEIPHETLLKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDIQMRRDGFADLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=701109 NF674_RS22145 WP_042610478.1 5006717..5008417(+) (pilB) [Pseudomonas moraviensis strain OTU5VILLAA1]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTCCAGGCCGAATTGCTCACGGAAACAAGCGCCCGGCAATC
CTGGGCGCAGGCCCAGCGCAATCGTGTGTCGCTGGTCAATTACCTGGTGCACAACAAACTGGTCAACAGCCGGCAGATCG
CCGAGATCGCCTCGGAACATTTCGGCATGGCGCTGCTCGATCTCAACTGCCTCGACAAGGAGACCCAGCCCAAGGGCCTG
GTCAGTGAAAAACTGGTGCGTCAGCACTGCGCCCTGCCCCTGTGGCGGCGTGGCAACAAATTGTTCGTGGGTCTATCCGA
CCCGAGCAATCAGCAGGCAATCAGCGACATCCAGTTCAGCACCGGCCTGAGCACCGAAGCGATTCTGGTCGAGGACGACA
AGCTGAGCGACGCCATCGACAAGTTCTTCGACAGCCATGCTTCGGGCCTGGAAGAAATGGCCGATGTCGATCTGGACGGC
CTCGACGTCGAGTCCATCGATGACAACAAGCAGGACGTTATCGGCGGCCTCGATGCGGACGATGCTCCAGTCGTGCGCTT
CGTGCACAAGATGCTGCTCGATGCGATCAAGAGCGGCTCTTCCGACCTGCATTTCGAGCCCTACGAGAAAAACTACCGGG
TACGCATGCGCACCGACGGCATGCTGCGCGAGGTGGCCAAGCCGCCGATCCAGTTGGCCGGGCGCATTGCCGCACGTTTG
AAGGTCATGGCCAGCCTCGATATTTCGGAACGGCGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCGAAAAGCAA
ATCCATCGATTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGTG
CGCAAATGGGCATCGACGCCTTGGGTTACGAGGCCGAACAAAAAGCCTTGTACCTCGCTGCGCTGAAACAGCCGCAAGGG
ATGATTCTGGTCACTGGCCCCACGGGCTCCGGTAAAACCGTGTCGCTGTACACCGGGCTGAACATCCTCAACACCGTCGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCCGCCAGG
GGCTGGATTTCGCTCAGGCCCTGCGTTCGTTTCTGCGTCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCGATCAAAGCTGCGCAGACCGGTCACCTGGTGCTATCGACCCTGCACACCAACAGCGCGGCGGA
AACTCTCACGCGCCTGCACAACATGGGTATTCCCGGTTTCAACATCGCCACCTCGGTGAGCCTGATCATCGCCCAGCGTC
TGGCACGCAAGTTATGTGCGCACTGCAAACGGCCGCTGGAGATCCCGCACGAAACCTTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGTCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAATGGCGGGTACAAAGGACGCGTGGGGAT
TTATGAAGTGGTAAAAAACACACCGGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCGCTGGAGATCGACATCCAGA
TGCGCCGCGACGGTTTCGCCGACCTGCGCACCTCCGGGCTGCACAAGGCCATGCAAGGCATCACCAGCCTTGAGGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.699

99.823

0.576
  pilB Acinetobacter baumannii D1279779

57.447

99.647

0.572
  pilB Legionella pneumophila strain ERS1305867

56.714

100

0.567
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50.446

99.117

0.5
  pilF Neisseria gonorrhoeae MS11

50

99.647

0.498
  pilB Vibrio campbellii strain DS40M4

49.385

100

0.496
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.065

94.523

0.369