Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NF679_RS22365 Genome accession   NZ_CP099601
Coordinates   4983713..4984930 (+) Length   405 a.a.
NCBI ID   WP_252868298.1    Uniprot ID   -
Organism   Pseudomonas siliginis strain OTU6MONEA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4978713..4989930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF679_RS22345 (NF679_22345) - 4980184..4980483 (+) 300 WP_095181833.1 DUF2845 domain-containing protein -
  NF679_RS22350 (NF679_22350) - 4980719..4981072 (-) 354 WP_252868297.1 BON domain-containing protein -
  NF679_RS22355 (NF679_22355) - 4981367..4981783 (-) 417 WP_056790436.1 pilin -
  NF679_RS22360 (NF679_22360) pilB 4982010..4983710 (+) 1701 WP_056790439.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NF679_RS22365 (NF679_22365) pilC 4983713..4984930 (+) 1218 WP_252868298.1 type II secretion system F family protein Machinery gene
  NF679_RS22370 (NF679_22370) pilD 4984932..4985804 (+) 873 WP_252868299.1 A24 family peptidase Machinery gene
  NF679_RS22375 (NF679_22375) coaE 4985801..4986424 (+) 624 WP_186569367.1 dephospho-CoA kinase -
  NF679_RS22380 (NF679_22380) yacG 4986421..4986621 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  NF679_RS22385 (NF679_22385) - 4986629..4986844 (-) 216 WP_016773148.1 hypothetical protein -
  NF679_RS22390 (NF679_22390) - 4986910..4987599 (-) 690 WP_252868300.1 energy-coupling factor ABC transporter permease -
  NF679_RS22395 (NF679_22395) - 4987790..4988416 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -
  NF679_RS22400 (NF679_22400) - 4988413..4988940 (+) 528 WP_252868301.1 MOSC domain-containing protein -
  NF679_RS22405 (NF679_22405) - 4988992..4989165 (+) 174 WP_007909875.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44029.69 Da        Isoelectric Point: 10.1214

>NTDB_id=700993 NF679_RS22365 WP_252868298.1 4983713..4984930(+) (pilC) [Pseudomonas siliginis strain OTU6MONEA1]
MAVKAAKVSVYAWEGTDRKGSKVSGELSGQNPALIKAQLRKQGINPGKVRKKSASLLSFGKRIKPQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
EALKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPGFTLMIISLSEFMQQWWWAILFALVAAFFGTR
HALKTSQALRDRRDAWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRVRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDDMLDKVAGFYEAEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=700993 NF679_RS22365 WP_252868298.1 4983713..4984930(+) (pilC) [Pseudomonas siliginis strain OTU6MONEA1]
ATGGCGGTCAAGGCAGCAAAAGTCAGCGTTTACGCCTGGGAAGGCACCGACCGCAAAGGCAGTAAAGTCAGCGGCGAGTT
GAGCGGACAGAACCCCGCACTGATCAAGGCGCAGCTGCGCAAACAGGGGATCAACCCGGGTAAGGTGCGCAAGAAATCCG
CGTCCCTGCTGAGCTTCGGCAAACGCATCAAACCGCAAGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGCGTGCCGCTGTTGCAGTCCTTCGACATCATCGGCGAAGGCTTCGAAAACCCGGCCATGCGCAAACTGGTCGACGA
GGTCAAACAGGAGGTCGCGGCCGGTAACAGCTTCGCTACGGCCCTGCGCAAGAAACCGCAATACTTCGACGAGTTGTACT
GCAATCTGGTCGACGCCGGCGAACAGTCTGGCGCCCTCGACACCTTGCTTGAACGGGTCGCGACCTATAAGGAAAAAAGC
GAAGCGCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCCACCGCCGTGGTGCTGGTGGCGGCAGTGGTAACAGGCAT
TCTGCTGGTCAAAGTGGTGCCGCAGTTCCAGTCGGTGTTTTCCGGGTTCGGCGCAGAACTGCCGGGCTTCACCCTGATGA
TCATCAGCCTGTCGGAATTCATGCAGCAGTGGTGGTGGGCCATTCTTTTTGCGCTGGTGGCAGCGTTTTTCGGCACTCGC
CACGCGTTGAAGACTTCGCAGGCCCTGCGCGACCGGCGCGATGCCTGGCTGCTGAAGCTGCCACTGGTGGGCACCTTGAT
GTACAAGTCCGCCGTCGCACGTTTTGCCCGCACCCTGTCGACCACTTTCGCAGCCGGTGTGCCGCTGGTGGAAGCGCTGG
ATTCGGTCGCCGGCGCCACCGGCAATGTGGTGTTCAAGCGCGCGGTGCTGCGCGTGCGGCAAGACGTCTCGACCGGCATG
CAACTGAATTTCTCCATGCGCAGCACCGGTGTGTTTCCCAACATGGCGGTGCAGATGACCGCCATCGGCGAGGAATCGGG
CGCGCTGGATGACATGCTCGACAAGGTCGCCGGTTTTTATGAAGCGGAAGTCGATAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGGGTCATCGTCGGTGGACTGGTGGTGGCCATGTACCTGCCGATCTTCCAG
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.321

100

0.743
  pilC Acinetobacter baylyi ADP1

58.824

100

0.593
  pilC Acinetobacter baumannii D1279779

58.578

100

0.59
  pilC Legionella pneumophila strain ERS1305867

52.75

98.765

0.521
  pilC Vibrio cholerae strain A1552

44.697

97.778

0.437
  pilG Neisseria gonorrhoeae MS11

43.424

99.506

0.432
  pilG Neisseria meningitidis 44/76-A

42.68

99.506

0.425
  pilC Vibrio campbellii strain DS40M4

41.626

100

0.417
  pilC Thermus thermophilus HB27

39.599

98.519

0.39