Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NF675_RS22145 Genome accession   NZ_CP099596
Coordinates   5053085..5054302 (+) Length   405 a.a.
NCBI ID   WP_041477818.1    Uniprot ID   -
Organism   Pseudomonas siliginis strain OTU6BAGNBB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5048085..5059302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NF675_RS22125 (NF675_22125) - 5049531..5049830 (+) 300 WP_252878910.1 DUF2845 domain-containing protein -
  NF675_RS22130 (NF675_22130) - 5050095..5050448 (-) 354 WP_252878911.1 BON domain-containing protein -
  NF675_RS22135 (NF675_22135) - 5050739..5051155 (-) 417 WP_071173630.1 pilin -
  NF675_RS22140 (NF675_22140) pilB 5051382..5053082 (+) 1701 WP_056790439.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NF675_RS22145 (NF675_22145) pilC 5053085..5054302 (+) 1218 WP_041477818.1 type II secretion system F family protein Machinery gene
  NF675_RS22150 (NF675_22150) pilD 5054304..5055176 (+) 873 WP_252878912.1 A24 family peptidase Machinery gene
  NF675_RS22155 (NF675_22155) coaE 5055173..5055796 (+) 624 WP_076564149.1 dephospho-CoA kinase -
  NF675_RS22160 (NF675_22160) yacG 5055793..5055993 (+) 201 WP_016773149.1 DNA gyrase inhibitor YacG -
  NF675_RS22165 (NF675_22165) - 5056001..5056216 (-) 216 WP_016773148.1 hypothetical protein -
  NF675_RS22170 (NF675_22170) - 5056282..5056971 (-) 690 WP_041477814.1 energy-coupling factor ABC transporter permease -
  NF675_RS22175 (NF675_22175) - 5057162..5057788 (+) 627 WP_007909887.1 DUF1780 domain-containing protein -
  NF675_RS22180 (NF675_22180) - 5057785..5058312 (+) 528 WP_160768590.1 MOSC domain-containing protein -
  NF675_RS22185 (NF675_22185) - 5058364..5058537 (+) 174 WP_007909875.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44043.72 Da        Isoelectric Point: 10.1214

>NTDB_id=700876 NF675_RS22145 WP_041477818.1 5053085..5054302(+) (pilC) [Pseudomonas siliginis strain OTU6BAGNBB1]
MAVKAAKVSVYAWEGTDRKGSKVSGELSGQNPALIKAQLRKQGINPGKVRKKSASLLTFGKRIKPQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
EALKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPGFTLMIISLSEFMQQWWWAILFALVAAFFGTR
HALKTSQALRDRRDAWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRVRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDDMLDKVAGFYEAEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=700876 NF675_RS22145 WP_041477818.1 5053085..5054302(+) (pilC) [Pseudomonas siliginis strain OTU6BAGNBB1]
ATGGCCGTCAAGGCAGCGAAAGTCAGCGTTTACGCCTGGGAAGGCACCGACCGCAAAGGCAGTAAAGTCAGCGGCGAGTT
GAGCGGACAGAACCCCGCACTGATCAAGGCGCAGCTGCGCAAACAGGGGATCAACCCGGGCAAGGTGCGCAAGAAATCCG
CGTCCCTGCTGACCTTCGGCAAACGCATCAAACCGCAGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCCGGCGTGCCGCTGTTGCAGTCCTTCGACATCATCGGCGAAGGCTTCGAAAACCCGGCCATGCGCAAACTGGTCGACGA
GGTCAAACAGGAGGTCGCGGCCGGTAACAGCTTCGCTACGGCCCTGCGCAAGAAACCGCAATACTTCGACGAGTTGTACT
GCAACCTGGTCGACGCCGGCGAACAGTCCGGTGCCCTCGACACTTTGCTCGAACGGGTCGCGACCTATAAGGAAAAAAGC
GAAGCGCTCAAGGCCAAGATCAAAAAAGCCATGACTTACCCTACTGCGGTGGTGCTGGTGGCGGCGGTGGTGACCGGCAT
TCTGCTGGTCAAAGTGGTGCCGCAGTTCCAGTCGGTATTCTCCGGGTTCGGCGCCGAACTGCCGGGCTTTACCCTGATGA
TCATCAGCCTGTCGGAGTTCATGCAGCAGTGGTGGTGGGCGATTCTTTTTGCGCTGGTGGCAGCGTTTTTCGGCACTCGC
CACGCGTTGAAGACTTCGCAGGCCCTGCGCGACCGCCGCGATGCCTGGCTGCTGAAGCTGCCGCTGGTAGGCACTTTGAT
GTACAAGTCCGCCGTCGCCCGCTTTGCCCGCACCCTGTCGACCACTTTCGCAGCCGGTGTGCCGCTGGTGGAAGCGCTGG
ATTCGGTCGCCGGCGCCACCGGCAATGTGGTGTTCAAGCGCGCGGTGTTGCGGGTGCGGCAGGACGTCTCGACCGGCATG
CAACTGAATTTCTCCATGCGCAGCACCGGTGTGTTTCCCAACATGGCGGTGCAGATGACCGCCATCGGCGAGGAATCGGG
CGCGCTGGATGACATGCTCGACAAGGTCGCCGGTTTTTATGAAGCGGAAGTCGATAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTGGGGGTCATCGTCGGTGGACTGGTGGTGGCCATGTACCTGCCGATCTTCCAG
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.074

100

0.741
  pilC Acinetobacter baylyi ADP1

58.824

100

0.593
  pilC Acinetobacter baumannii D1279779

58.578

100

0.59
  pilC Legionella pneumophila strain ERS1305867

52.75

98.765

0.521
  pilC Vibrio cholerae strain A1552

44.697

97.778

0.437
  pilG Neisseria gonorrhoeae MS11

43.424

99.506

0.432
  pilG Neisseria meningitidis 44/76-A

42.68

99.506

0.425
  pilC Vibrio campbellii strain DS40M4

41.872

100

0.42
  pilC Thermus thermophilus HB27

39.599

98.519

0.39