Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NG827_RS06785 Genome accession   NZ_CP099533
Coordinates   1628065..1629195 (+) Length   376 a.a.
NCBI ID   WP_010344137.1    Uniprot ID   -
Organism   Xanthomonas sacchari strain LT6-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1623065..1634195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG827_RS06760 (NG827_06750) - 1623208..1623660 (-) 453 WP_267098247.1 DUF4426 domain-containing protein -
  NG827_RS06765 (NG827_06755) proC 1623803..1624648 (-) 846 WP_267098248.1 pyrroline-5-carboxylate reductase -
  NG827_RS06770 (NG827_06760) - 1624777..1625805 (-) 1029 WP_318526745.1 hypothetical protein -
  NG827_RS06775 (NG827_06765) - 1626122..1626778 (-) 657 WP_267098636.1 YggS family pyridoxal phosphate-dependent enzyme -
  NG827_RS06780 (NG827_06770) pilT 1626900..1627937 (+) 1038 WP_010344138.1 type IV pilus twitching motility protein PilT Machinery gene
  NG827_RS06785 (NG827_06775) pilU 1628065..1629195 (+) 1131 WP_010344137.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NG827_RS06790 (NG827_06780) - 1629310..1630599 (+) 1290 WP_267098250.1 WD40 repeat domain-containing protein -
  NG827_RS06795 (NG827_06785) - 1630596..1632095 (+) 1500 WP_267098251.1 diguanylate cyclase -
  NG827_RS06800 (NG827_06790) - 1632446..1633042 (+) 597 WP_267098252.1 MFS transporter permease -
  NG827_RS06805 (NG827_06795) - 1633195..1633911 (+) 717 WP_267098253.1 YitT family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41906.19 Da        Isoelectric Point: 6.8811

>NTDB_id=700456 NG827_RS06785 WP_010344137.1 1628065..1629195(+) (pilU) [Xanthomonas sacchari strain LT6-2]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKITPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPSVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRVINFFPEDRRSQLLMDLSLNLRGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDARTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=700456 NG827_RS06785 WP_010344137.1 1628065..1629195(+) (pilU) [Xanthomonas sacchari strain LT6-2]
ATGAGCACCATCGACTTCACCTCCTTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCTCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCACCCCGATCACGCAGACGCCGCTGACCCCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACGCCGGCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGT
GTGGGGCGCTTCCGCGTCAGTTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGCATTCC
CAGCGTGGACGAGCTGAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCCACCGGGCACATCATCACC
ATCGAGGACCCGATTGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGATAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGACCATGCCATCTCCTTCGCCGAGACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCGTCATCAACTTCTTCCCCGAGGACCGCCGCAGCCAGCTGCTGATGGACCTGTCGCTGAACCTGCGCGGCGTGGT
CGCGCAGCAGCTGATCCCGACCCCGGACGGCAAGGGCCGCCGCGTGGCCATGGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGAGGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAGTCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCCACGCCGATTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGGCGCAGGGCGGCGACGCGCGCACCCTGGCGCAGGGCCTGGACGGGGTCGAGATCGCCG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.485

97.606

0.649
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42

93.085

0.391
  pilT Pseudomonas aeruginosa PAK

41.003

90.16

0.37
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Legionella pneumophila strain ERS1305867

40.597

89.096

0.362
  pilT Legionella pneumophila strain Lp02

40.597

89.096

0.362