Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   PCP36_RS08245 Genome accession   NZ_CP115227
Coordinates   1728215..1728679 (-) Length   154 a.a.
NCBI ID   WP_134236867.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain F052     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1723215..1733679
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP36_RS08230 (PCP36_08225) nadC 1725736..1726584 (+) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PCP36_RS08240 (PCP36_08235) - 1726765..1728123 (-) 1359 WP_226342803.1 O-antigen ligase family protein -
  PCP36_RS08245 (PCP36_08240) pilA/pilAI 1728215..1728679 (-) 465 WP_134236867.1 pilin Machinery gene
  PCP36_RS08250 (PCP36_08245) pilB 1728910..1730610 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCP36_RS08255 (PCP36_08250) pilC 1730614..1731831 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  PCP36_RS08260 (PCP36_08255) pilD 1731832..1732704 (+) 873 WP_174216249.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PCP36_RS08265 (PCP36_08260) coaE 1732701..1733312 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  PCP36_RS08270 (PCP36_08265) yacG 1733309..1733509 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16235.55 Da        Isoelectric Point: 8.4605

>NTDB_id=697238 PCP36_RS08245 WP_134236867.1 1728215..1728679(-) (pilA/pilAI) [Pseudomonas aeruginosa strain F052]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSKTPNDTQYDIGFTE
STLLDGSGKSQIQVTDNKNGTVELVATLGKSSGSAIKGAVITVSRDAQGVWSCNITKTPTAWKPNYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=697238 PCP36_RS08245 WP_134236867.1 1728215..1728679(-) (pilA/pilAI) [Pseudomonas aeruginosa strain F052]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCTCGTACCCAGGTGACCCGTGCCGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAGGAGATTGTTTCCAGCAAGACTCCTAACGATACCCAGTATGACATCGGCTTCACCGAG
TCTACTTTGCTAGATGGTTCTGGTAAGAGTCAGATCCAGGTAACGGACAATAAAAATGGCACCGTTGAGTTGGTCGCTAC
CTTGGGTAAATCTTCTGGTTCCGCCATCAAAGGGGCTGTCATCACTGTTTCGCGTGACGCTCAAGGAGTCTGGAGCTGCA
ATATCACCAAAACTCCTACAGCTTGGAAGCCCAACTACGCTCCGGCTAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.444

99.351

0.442
  pilA Acinetobacter baumannii strain A118

43.421

98.701

0.429
  comP Acinetobacter baylyi ADP1

39.634

100

0.422
  pilA Pseudomonas aeruginosa PAK

41.401

100

0.422
  pilA Ralstonia pseudosolanacearum GMI1000

35.955

100

0.416
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.065

100

0.383
  pilA Vibrio cholerae strain A1552

38.065

100

0.383
  pilA Vibrio cholerae C6706

38.065

100

0.383
  pilA Vibrio parahaemolyticus RIMD 2210633

39.333

97.403

0.383
  pilA2 Legionella pneumophila strain ERS1305867

38.411

98.052

0.377