Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   SR187_RS00795 Genome accession   NZ_AP018400
Coordinates   124422..125453 (+) Length   343 a.a.
NCBI ID   WP_120171205.1    Uniprot ID   -
Organism   Streptococcus ruminantium strain GUT-187     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 119422..130453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SR187_RS00780 (SR187_0805) - 122033..122401 (+) 369 WP_120171203.1 DUF1033 family protein -
  SR187_RS00785 (SR187_0810) - 122439..123389 (-) 951 WP_024532257.1 S66 peptidase family protein -
  SR187_RS00790 (SR187_0815) comYA 123551..124501 (+) 951 WP_120171204.1 competence type IV pilus ATPase ComGA Machinery gene
  SR187_RS00795 (SR187_0820) comYB 124422..125453 (+) 1032 WP_120171205.1 competence type IV pilus assembly protein ComGB Machinery gene
  SR187_RS00800 (SR187_0825) comYC 125450..125731 (+) 282 WP_024532254.1 competence type IV pilus major pilin ComGC Machinery gene
  SR187_RS00805 (SR187_0830) comGD 125718..126131 (+) 414 WP_024532253.1 competence type IV pilus minor pilin ComGD -
  SR187_RS00810 (SR187_0835) comYE 126103..126396 (+) 294 WP_120172446.1 competence type IV pilus minor pilin ComGE Machinery gene
  SR187_RS00815 (SR187_0840) comGF/cglF 126383..126817 (+) 435 WP_120171206.1 competence type IV pilus minor pilin ComGF Machinery gene
  SR187_RS00820 (SR187_0845) comGG 126795..127208 (+) 414 WP_120171207.1 competence type IV pilus minor pilin ComGG -
  SR187_RS00825 (SR187_0850) comYH 127213..128166 (+) 954 WP_120171208.1 class I SAM-dependent methyltransferase Machinery gene
  SR187_RS00830 (SR187_0855) - 128216..129403 (+) 1188 WP_120171209.1 acetate kinase -
  SR187_RS00835 (SR187_0860) - 129708..130262 (+) 555 WP_120171210.1 folate family ECF transporter S component -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 38998.90 Da        Isoelectric Point: 8.7768

>NTDB_id=69428 SR187_RS00795 WP_120171205.1 124422..125453(+) (comYB) [Streptococcus ruminantium strain GUT-187]
MIAFLQQDISVIGRQRQKRLSLDHQRKVIELFNNLFASGFHLGEIVDFLRRSQLLADRYTQVLSDGLLAGKPFSSLLADL
RFSDAVVTQVSLAEVHGNTSLSLFHIQSYLENVSKIRKKLIEVATYPVILLAFLLLIVVGLKNYLLPQLEEENIATVLIN
HLPAIFLSVVFLLSVGVLAGLTCYRKASKIKVFSRLVALPFLGKVIQTYLTAYYAREWGSLIGQGLEMPQIVELMQTQRS
QLFREIGEDLESALSNGQSFHYHIQSYPFFKRELSLIIEYGQVKSKLGSELALYAAECWEEFFSRVNRAMQLIQPLVFLF
VALMVVLIYAAMLLPIYQNMELK

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=69428 SR187_RS00795 WP_120171205.1 124422..125453(+) (comYB) [Streptococcus ruminantium strain GUT-187]
TTGATAGCCTTTTTGCAGCAGGATATATCAGTCATAGGCAGGCAGAGACAGAAAAGATTGTCTTTGGATCACCAGCGTAA
GGTGATTGAATTATTTAACAATCTCTTTGCAAGTGGTTTTCATTTGGGAGAAATTGTGGATTTTCTCAGGCGCAGTCAGC
TCCTAGCGGACCGTTACACACAGGTTTTATCAGATGGACTGTTGGCTGGTAAACCATTTTCTAGCCTTCTAGCGGATCTT
CGTTTTTCGGATGCTGTTGTCACTCAGGTTTCTCTTGCAGAAGTTCATGGTAATACTAGTTTGAGTCTGTTCCACATCCA
GTCCTATCTGGAAAATGTCAGTAAAATCCGTAAAAAATTGATCGAGGTTGCCACTTATCCAGTCATTTTACTGGCTTTTC
TTCTCCTGATTGTAGTAGGCTTGAAAAACTATCTCTTGCCGCAATTGGAAGAAGAAAACATAGCAACGGTGTTGATTAAC
CACTTACCAGCTATTTTTTTATCCGTAGTTTTCTTATTATCGGTAGGAGTTTTAGCAGGTCTTACTTGCTATCGCAAAGC
GAGCAAAATCAAGGTCTTTTCACGCTTGGTTGCTTTGCCATTTTTGGGAAAAGTTATCCAAACCTATCTAACAGCGTATT
ATGCTAGGGAGTGGGGAAGTCTAATCGGTCAGGGCTTAGAAATGCCACAGATTGTGGAGTTGATGCAGACACAGCGGTCG
CAATTATTCAGAGAGATTGGAGAAGATTTAGAATCAGCTCTTTCAAATGGTCAGAGCTTCCATTATCATATTCAGTCCTA
TCCATTTTTCAAGCGAGAGTTGAGTTTGATTATTGAGTATGGACAAGTCAAATCCAAACTGGGAAGCGAGTTAGCATTGT
ATGCAGCGGAGTGTTGGGAAGAATTTTTCTCCCGAGTCAATAGGGCCATGCAGTTGATTCAACCGCTAGTCTTTCTCTTT
GTAGCCTTGATGGTCGTTCTTATCTACGCAGCCATGTTGCTGCCTATTTATCAGAATATGGAGTTAAAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(30-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus gordonii str. Challis substr. CH1

63.158

99.708

0.63
  comGB/cglB Streptococcus mitis NCTC 12261

61.012

97.959

0.598
  comYB Streptococcus mutans UA159

58.43

100

0.586
  comYB Streptococcus mutans UA140

58.43

100

0.586
  comGB/cglB Streptococcus pneumoniae Rx1

59.821

97.959

0.586
  comGB/cglB Streptococcus pneumoniae R6

59.821

97.959

0.586
  comGB/cglB Streptococcus pneumoniae TIGR4

59.821

97.959

0.586
  comGB/cglB Streptococcus pneumoniae D39

59.821

97.959

0.586
  comGB/cglB Streptococcus mitis SK321

59.524

97.959

0.583
  comGB Lactococcus lactis subsp. cremoris KW2

49.852

98.251

0.49

Multiple sequence alignment