Detailed information NTG0069431

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: SR187_RS00815
• Genome accession: NZ_AP018400
• Coordinates: 126383..126817 (+)
• Length: 144 a.a.
• NCBI ID: WP_120171206.1
• Uniprot ID: -
• Organism: Streptococcus ruminantium strain GUT-187
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 121383..131817

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SR187_RS00780 (SR187_0805)	-	122033..122401 (+)	369	WP_120171203.1	DUF1033 family protein	-
SR187_RS00785 (SR187_0810)	-	122439..123389 (-)	951	WP_024532257.1	S66 peptidase family protein	-
SR187_RS00790 (SR187_0815)	comYA	123551..124501 (+)	951	WP_120171204.1	competence type IV pilus ATPase ComGA	Machinery gene
SR187_RS00795 (SR187_0820)	comYB	124422..125453 (+)	1032	WP_120171205.1	competence type IV pilus assembly protein ComGB	Machinery gene
SR187_RS00800 (SR187_0825)	comYC	125450..125731 (+)	282	WP_024532254.1	competence type IV pilus major pilin ComGC	Machinery gene
SR187_RS00805 (SR187_0830)	comGD	125718..126131 (+)	414	WP_024532253.1	competence type IV pilus minor pilin ComGD	-
SR187_RS00810 (SR187_0835)	comYE	126103..126396 (+)	294	WP_120172446.1	competence type IV pilus minor pilin ComGE	Machinery gene
SR187_RS00815 (SR187_0840)	comGF/cglF	126383..126817 (+)	435	WP_120171206.1	competence type IV pilus minor pilin ComGF	Machinery gene
SR187_RS00820 (SR187_0845)	comGG	126795..127208 (+)	414	WP_120171207.1	competence type IV pilus minor pilin ComGG	-
SR187_RS00825 (SR187_0850)	comYH	127213..128166 (+)	954	WP_120171208.1	class I SAM-dependent methyltransferase	Machinery gene
SR187_RS00830 (SR187_0855)	-	128216..129403 (+)	1188	WP_120171209.1	acetate kinase	-
SR187_RS00835 (SR187_0860)	-	129708..130262 (+)	555	WP_120171210.1	folate family ECF transporter S component	-
SR187_RS00840 (SR187_0865)	-	130361..131617 (+)	1257	WP_120171211.1	folylpolyglutamate synthase/dihydrofolate synthase family protein	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16487.97 Da
• Isoelectric Point: 8.8413

>NTDB_id=69431 SR187_RS00815 WP_120171206.1 126383..126817(+) (comGF/cglF) [Streptococcus ruminantium strain GUT-187]
MLKTRAPAFTLLECLVALVILSGSLLVFEGLSKLISHEVRYQSKVLQKDWLLFSDQLRAEWAQAALVRVENNRIYINKEG
QGLAFGKSRSDDFRKTNEKGQGYQPMLYGLQATEIVQEGRLVRMDFTFTNGEERTFIYAFEKTG

Nucleotide

• Length: 435 bp

>NTDB_id=69431 SR187_RS00815 WP_120171206.1 126383..126817(+) (comGF/cglF) [Streptococcus ruminantium strain GUT-187]
TTGCTAAAAACTAGGGCTCCAGCTTTTACCCTTTTAGAATGTTTAGTGGCTTTGGTCATCCTGTCGGGGAGTCTCTTAGT
ATTTGAAGGATTAAGCAAATTGATTTCTCATGAAGTTCGTTACCAAAGCAAGGTACTTCAAAAGGATTGGCTCCTTTTCT
CGGATCAGCTGCGTGCAGAATGGGCTCAGGCGGCTTTGGTTAGAGTTGAGAATAACAGAATCTACATCAATAAGGAAGGT
CAAGGCCTTGCCTTTGGAAAATCACGTTCGGATGATTTTCGGAAGACGAATGAGAAGGGGCAAGGGTATCAGCCTATGTT
ATACGGTCTTCAAGCGACGGAAATTGTTCAAGAGGGAAGACTAGTTAGAATGGATTTTACCTTTACAAATGGAGAGGAGC
GAACTTTTATCTATGCTTTTGAAAAAACAGGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (40-134)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus mitis NCTC 12261	52.518	96.528	0.507
comGF/cglF	Streptococcus pneumoniae Rx1	52.174	95.833	0.5
comGF/cglF	Streptococcus pneumoniae D39	52.174	95.833	0.5
comGF/cglF	Streptococcus pneumoniae R6	52.174	95.833	0.5
comGF/cglF	Streptococcus pneumoniae TIGR4	52.174	95.833	0.5
comGF/cglF	Streptococcus mitis SK321	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	49.645	97.917	0.486
comYF	Streptococcus mutans UA140	48.936	97.917	0.479
comGF	Lactococcus lactis subsp. cremoris KW2	45.775	98.611	0.451