Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CKCBHOJB_RS14865 Genome accession   NZ_CP097870
Coordinates   3225061..3226290 (+) Length   409 a.a.
NCBI ID   WP_281049435.1    Uniprot ID   -
Organism   Thauera sp. GDN1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3220061..3231290
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKCBHOJB_RS14845 (CKCBHOJB_02961) - 3220199..3220975 (-) 777 WP_281049431.1 EI24 domain-containing protein -
  CKCBHOJB_RS14850 (CKCBHOJB_02962) ffh 3220992..3222362 (-) 1371 WP_281049432.1 signal recognition particle protein -
  CKCBHOJB_RS14855 (CKCBHOJB_02963) ccsA 3222411..3223244 (+) 834 WP_281049433.1 cytochrome c biogenesis protein CcsA -
  CKCBHOJB_RS14860 (CKCBHOJB_02964) pilB 3223330..3225045 (+) 1716 WP_281049434.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CKCBHOJB_RS14865 (CKCBHOJB_02965) pilC 3225061..3226290 (+) 1230 WP_281049435.1 type II secretion system F family protein Machinery gene
  CKCBHOJB_RS14870 (CKCBHOJB_02966) pilD 3226294..3227145 (+) 852 WP_281049436.1 A24 family peptidase Machinery gene
  CKCBHOJB_RS14875 (CKCBHOJB_02967) - 3227278..3227541 (+) 264 WP_281049437.1 zinc ribbon domain-containing protein -
  CKCBHOJB_RS14880 (CKCBHOJB_02968) - 3227543..3228169 (+) 627 WP_281049438.1 DUF502 domain-containing protein -
  CKCBHOJB_RS14885 (CKCBHOJB_02969) aspS 3228254..3230053 (+) 1800 WP_281049439.1 aspartate--tRNA ligase -
  CKCBHOJB_RS14890 (CKCBHOJB_02970) - 3230139..3230534 (+) 396 WP_281049440.1 ribonuclease domain-containing protein -
  CKCBHOJB_RS14895 (CKCBHOJB_02971) - 3230531..3230914 (+) 384 WP_281049441.1 barstar family protein -
  CKCBHOJB_RS14900 (CKCBHOJB_02972) - 3230853..3231086 (-) 234 WP_348634850.1 cysteine-rich CWC family protein -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44218.21 Da        Isoelectric Point: 9.8184

>NTDB_id=693177 CKCBHOJB_RS14865 WP_281049435.1 3225061..3226290(+) (pilC) [Thauera sp. GDN1]
MATASRAMRATGPKEELYTWEGKDKTGKVVRGEIRANGDAMVQAMLRRQGIQVGKIKKLKMSRGGRITDKDIALFTRQLA
TMMKSGVPLLQAFDIAMKGSGNPALSRLLNEIRTDVETGLNLSQAFAKHPVHFDKLFCNLVAAGEQAGILDSLLDRIATY
KEKILAIKGKIKSALFYPAAVVVVAALVISVMMLFVIPEFKNVFSSFGADLPAPTLLVIAMSDFFVDYWYVIFGVLIGAI
VATAWSYKRSTAMQTTLDRLVLRLPVIGPIIRKATIARWTRTLSTMFAAGVPLVEALDSVGGAAGNHVYMVATKQIQSEV
STGTSLTVAMQNADVFPTMVVQMVSIGEESGQLDGMLGKVADFFEQEVDDAVAGLSQLLEPIIMVFLGTIIGGLVVAMYL
PIFKLGSVI

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=693177 CKCBHOJB_RS14865 WP_281049435.1 3225061..3226290(+) (pilC) [Thauera sp. GDN1]
ATGGCGACTGCGAGCCGGGCGATGCGCGCCACAGGCCCCAAGGAAGAGCTGTACACCTGGGAAGGCAAGGACAAGACCGG
CAAGGTCGTGCGCGGCGAGATCCGCGCCAACGGGGACGCCATGGTGCAGGCCATGCTGCGCCGCCAGGGCATCCAGGTCG
GCAAGATCAAGAAGCTGAAGATGTCGCGCGGCGGGCGCATCACCGACAAGGACATCGCGCTGTTCACCCGCCAGCTCGCC
ACCATGATGAAGTCCGGCGTGCCGCTGCTGCAGGCCTTCGACATCGCCATGAAGGGCTCGGGCAATCCGGCGCTTTCGCG
CCTGCTCAACGAGATCCGCACCGACGTCGAGACCGGCCTCAACCTGTCGCAGGCCTTCGCCAAGCATCCGGTCCATTTCG
ACAAGCTGTTCTGCAACCTGGTCGCCGCCGGCGAACAGGCCGGCATCCTGGACAGCCTGCTAGACCGCATCGCCACCTAC
AAGGAAAAGATCCTCGCCATCAAGGGCAAGATCAAGTCCGCGCTGTTCTACCCGGCCGCGGTCGTGGTGGTCGCCGCGCT
GGTGATCTCGGTGATGATGCTGTTCGTGATTCCCGAGTTCAAGAACGTGTTCTCCAGCTTCGGCGCCGATCTGCCGGCGC
CGACCCTGCTGGTGATCGCGATGTCGGACTTCTTCGTCGACTACTGGTACGTCATATTCGGCGTCCTGATCGGGGCGATC
GTCGCCACCGCCTGGAGCTACAAGCGCTCCACCGCCATGCAGACCACGCTCGACCGCCTGGTGCTGCGCCTGCCGGTGAT
CGGCCCGATCATCCGCAAGGCCACGATCGCACGCTGGACGCGCACGCTGTCGACGATGTTCGCCGCCGGCGTGCCGCTGG
TGGAGGCGCTGGACTCGGTGGGTGGCGCCGCCGGCAACCACGTCTACATGGTGGCGACCAAGCAGATCCAGAGCGAGGTC
AGCACCGGCACCAGCCTCACCGTCGCCATGCAGAATGCGGACGTGTTCCCCACCATGGTGGTGCAGATGGTGTCGATCGG
CGAGGAATCCGGCCAGCTCGACGGCATGCTCGGCAAGGTCGCCGACTTCTTCGAGCAGGAGGTCGACGACGCCGTCGCCG
GCCTGTCGCAACTGCTCGAGCCGATCATCATGGTGTTCCTGGGCACCATCATCGGCGGCCTGGTGGTGGCGATGTACCTG
CCGATCTTCAAGCTCGGCTCGGTCATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

53.448

99.267

0.531
  pilC Pseudomonas stutzeri DSM 10701

50.627

97.555

0.494
  pilC Acinetobacter baylyi ADP1

50.251

97.311

0.489
  pilG Neisseria gonorrhoeae MS11

50.126

97.066

0.487
  pilG Neisseria meningitidis 44/76-A

49.874

97.066

0.484
  pilC Acinetobacter baumannii D1279779

47.355

97.066

0.46
  pilC Vibrio cholerae strain A1552

42.172

96.822

0.408
  pilC Vibrio campbellii strain DS40M4

39.901

99.267

0.396
  pilC Thermus thermophilus HB27

39.152

98.044

0.384