Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CKCBHOJB_RS14860 Genome accession   NZ_CP097870
Coordinates   3223330..3225045 (+) Length   571 a.a.
NCBI ID   WP_281049434.1    Uniprot ID   -
Organism   Thauera sp. GDN1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3218330..3230045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKCBHOJB_RS14835 (CKCBHOJB_02959) - 3218680..3219456 (-) 777 WP_281049429.1 SprT family zinc-dependent metalloprotease -
  CKCBHOJB_RS14840 (CKCBHOJB_02960) - 3219453..3220202 (-) 750 WP_281049430.1 molybdopterin-binding protein -
  CKCBHOJB_RS14845 (CKCBHOJB_02961) - 3220199..3220975 (-) 777 WP_281049431.1 EI24 domain-containing protein -
  CKCBHOJB_RS14850 (CKCBHOJB_02962) ffh 3220992..3222362 (-) 1371 WP_281049432.1 signal recognition particle protein -
  CKCBHOJB_RS14855 (CKCBHOJB_02963) ccsA 3222411..3223244 (+) 834 WP_281049433.1 cytochrome c biogenesis protein CcsA -
  CKCBHOJB_RS14860 (CKCBHOJB_02964) pilB 3223330..3225045 (+) 1716 WP_281049434.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CKCBHOJB_RS14865 (CKCBHOJB_02965) pilC 3225061..3226290 (+) 1230 WP_281049435.1 type II secretion system F family protein Machinery gene
  CKCBHOJB_RS14870 (CKCBHOJB_02966) pilD 3226294..3227145 (+) 852 WP_281049436.1 A24 family peptidase Machinery gene
  CKCBHOJB_RS14875 (CKCBHOJB_02967) - 3227278..3227541 (+) 264 WP_281049437.1 zinc ribbon domain-containing protein -
  CKCBHOJB_RS14880 (CKCBHOJB_02968) - 3227543..3228169 (+) 627 WP_281049438.1 DUF502 domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62114.49 Da        Isoelectric Point: 5.2254

>NTDB_id=693176 CKCBHOJB_RS14860 WP_281049434.1 3223330..3225045(+) (pilB) [Thauera sp. GDN1]
MAATTKPTLSGFARALIQHGHLQEADAVTCSAQAGDTTSAFMLEVANRGLMSCAAMARFAAETFGYPLLDITAFDPALFA
REAVDRKLLAKHQVAPLLKRQNRITLAVADPSNLRALDEIRFQTGMQLELVIAEADKLKRVVDTLSESAADTLKELTGEA
FDMDMLQQDGPTPQRDEDEANEVDDAPVVKFIQKVLIDAINEGASDVHFEPYEKFYRIRVRTDGILRDVAQPPLVLKDKI
AARIKVISRLDISEKRVPQDGRMKLVLSRNKAIDFRVSTLPTLHGEKIVMRILDPSSAMLGIDALGYDPDQKEALLAAIE
RPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNISTAEDPAEINLAGINQVNVNEKAGLTFSTALRAFLRQDPDVIMVGE
IRDLETAEISIKAAQTGHLVMSTVHTNDAPATLERLKNMGVAPFNIASSVILITAQRLARRLCSCKKPVDIPVEALIEAG
FGPEELDGSWQTMGPVGCDRCKGTGYKGRVGIYQVMPITEEIARIIMTGGNSMDIAAQAQREGVLDLRQSGLRKVKQGVT
SLEEVLATTNE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=693176 CKCBHOJB_RS14860 WP_281049434.1 3223330..3225045(+) (pilB) [Thauera sp. GDN1]
ATGGCGGCAACCACCAAACCGACCCTGAGCGGATTCGCCAGGGCGCTGATCCAGCATGGACATCTGCAGGAAGCGGATGC
CGTCACCTGCTCCGCACAGGCGGGTGACACCACCAGCGCCTTCATGCTCGAGGTCGCCAACCGCGGCCTGATGAGCTGTG
CCGCAATGGCGCGCTTCGCTGCCGAGACCTTCGGCTACCCGCTCCTGGACATCACCGCCTTCGACCCCGCGCTGTTCGCC
CGCGAGGCGGTCGACCGCAAGCTGCTGGCCAAGCACCAGGTCGCCCCACTGCTCAAGCGCCAGAACCGGATCACGCTCGC
GGTCGCCGATCCCTCGAACCTGCGCGCCCTCGACGAGATCCGCTTCCAGACCGGCATGCAGCTCGAGCTGGTCATCGCCG
AGGCGGACAAGCTCAAGCGCGTGGTCGACACCCTGTCCGAATCGGCCGCCGACACGCTCAAGGAGCTGACCGGCGAAGCC
TTCGACATGGACATGCTGCAGCAGGACGGGCCGACGCCGCAGCGCGACGAGGACGAGGCCAACGAGGTCGATGACGCCCC
GGTCGTCAAGTTCATCCAGAAGGTGCTGATCGACGCGATCAACGAGGGCGCATCCGACGTCCACTTCGAGCCCTACGAGA
AGTTCTACCGCATCCGCGTGCGCACCGACGGCATCCTGCGCGACGTCGCCCAGCCGCCGCTGGTGCTGAAGGACAAGATC
GCCGCACGCATCAAGGTGATCTCGCGCCTGGACATCTCCGAGAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTGGTGCT
GTCGCGGAACAAGGCGATCGACTTCCGGGTATCGACGCTGCCCACGCTGCACGGCGAGAAGATCGTCATGCGCATCCTCG
ACCCGAGCTCGGCCATGCTCGGCATCGACGCGCTCGGCTACGACCCCGATCAGAAGGAGGCCCTGCTCGCCGCCATCGAG
CGCCCCTACGGCATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTCTACACCTGCCTGAACCTGCT
CAACAAGCCCGGCGTGAACATCTCCACTGCCGAGGATCCGGCGGAAATCAACCTCGCCGGCATCAACCAGGTCAACGTCA
ACGAAAAGGCCGGCCTGACCTTCTCCACCGCGCTCCGCGCCTTCCTCCGCCAGGACCCGGACGTCATCATGGTGGGCGAG
ATCCGCGACCTCGAGACCGCCGAGATCTCGATCAAGGCGGCGCAGACCGGCCACCTGGTGATGTCCACCGTGCATACCAA
CGACGCCCCGGCGACGCTGGAACGGCTGAAGAACATGGGCGTGGCGCCGTTCAATATCGCCTCGTCGGTGATTCTTATCA
CGGCCCAGCGCCTCGCCCGCCGGCTATGCTCCTGCAAGAAGCCGGTGGACATCCCGGTCGAGGCGCTGATCGAGGCTGGA
TTCGGCCCCGAAGAGCTCGACGGCAGCTGGCAGACGATGGGCCCGGTGGGCTGCGATCGCTGCAAGGGCACGGGCTACAA
GGGCCGCGTCGGCATCTACCAGGTCATGCCGATCACCGAGGAAATCGCCCGCATCATCATGACGGGCGGCAACTCGATGG
ACATCGCGGCCCAGGCCCAGCGCGAGGGCGTGCTGGACCTGCGCCAGTCGGGACTGCGCAAGGTGAAACAGGGCGTCACC
TCACTCGAAGAAGTGCTGGCGACGACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.652

100

0.56
  pilB Acinetobacter baylyi ADP1

55.497

100

0.557
  pilB Legionella pneumophila strain ERS1305867

52.205

99.299

0.518
  pilF Neisseria gonorrhoeae MS11

52.12

99.124

0.517
  pilB Vibrio cholerae strain A1552

49.455

96.322

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

49.234

91.419

0.45
  pilB Vibrio campbellii strain DS40M4

48.381

91.944

0.445
  pilF Thermus thermophilus HB27

40.678

92.995

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.341

92.469

0.373