Detailed information NTG0687105

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M3M50_RS11865
• Genome accession: NZ_CP097108
• Coordinates: 2657188..2658303 (+)
• Length: 371 a.a.
• NCBI ID: WP_249016287.1
• Uniprot ID: -
• Organism: Pseudomonas bijieensis strain SP1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2652188..2663303

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M3M50_RS11850 (M3M50_11850)	-	2653135..2654055 (+)	921	WP_163854430.1	sugar kinase	-
M3M50_RS11855 (M3M50_11855)	-	2654194..2654469 (-)	276	WP_003182808.1	peptidylprolyl isomerase	-
M3M50_RS11860 (M3M50_11860)	-	2654518..2657055 (-)	2538	WP_232200183.1	PAS domain-containing protein	-
M3M50_RS11865 (M3M50_11865)	pilU	2657188..2658303 (+)	1116	WP_249016287.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
M3M50_RS11870 (M3M50_11870)	-	2658468..2660423 (-)	1956	WP_116832005.1	acetoacetate--CoA ligase	-
M3M50_RS11875 (M3M50_11875)	hbdH	2660581..2661357 (-)	777	WP_116832006.1	3-hydroxybutyrate dehydrogenase	-
M3M50_RS11880 (M3M50_11880)	-	2661365..2662756 (-)	1392	WP_109751812.1	GntP family permease	-

Sequence

Protein

• Length: 371 a.a.
• Molecular weight: 41200.18 Da
• Isoelectric Point: 6.4242

>NTDB_id=687105 M3M50_RS11865 WP_249016287.1 2657188..2658303(+) (pilU) [Pseudomonas bijieensis strain SP1]
MEIDPLLRILASQNGSDLYMSTGAPPCARFEGVLKPLGNEACKVGDIARLAESLMDAEQRLEFDRELEMNLAISLAGVGR
FRVNIFKQRNDVSMVIRNVKLDIPRFEDLKLPRVLLNTIMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTRTGQRRAAVEVMLGSPTVADLIRRNELGELKGIMEKSEELGMQTFDQAL
FNLVAEGAIDEEEALKNADSANNLRLRLKLHAESGAAPPADPAAGEWGLMD

Nucleotide

• Length: 1116 bp

>NTDB_id=687105 M3M50_RS11865 WP_249016287.1 2657188..2658303(+) (pilU) [Pseudomonas bijieensis strain SP1]
ATGGAAATCGATCCGTTGTTGCGAATCCTGGCGAGCCAGAATGGTTCCGATCTTTACATGTCCACGGGGGCGCCACCGTG
CGCGCGTTTCGAGGGCGTGCTCAAGCCCTTGGGTAATGAAGCATGCAAGGTCGGCGACATTGCCAGGCTCGCCGAGTCTT
TGATGGACGCCGAACAGCGCCTTGAGTTCGATCGGGAGCTGGAAATGAACCTGGCGATCTCCCTGGCCGGCGTCGGGCGT
TTCCGGGTCAATATCTTCAAACAACGCAATGACGTGTCCATGGTGATACGCAACGTCAAGCTGGACATCCCGCGTTTCGA
AGACCTGAAACTGCCACGGGTGCTGCTCAATACCATCATGCAGAAGCAAGGGCTGATGCTGTTCGTCGGAGCGACGGGGT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGATTACCGCAACCGCAACAGCAGCGGCCACATCATCACCATCGAAGAC
CCGGTGGAGTATATCCATCGGCACAAGAAATCCATCATCAACCAGCGGGAGGTCGGTGTCGACACCCGCAGTTTTCATGC
CGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGGTACTGATCGGCGAGATCCGCGACCGGGAAACCATGGAGCATG
CCTTGGCGTTTGCCGACACCGGGCACTTGGTGATTTCCACGTTGCATGCCCACAACGCCAACCAGGCCCTGGACCGGGTA
ATCAACTTCTTCCCGGAAGAGCGTCGGCCGCAGTTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTTTCCCAACG
CCTGGTGCGCACCCGTACCGGCCAGCGTCGGGCGGCGGTGGAGGTGATGCTGGGCTCGCCCACGGTGGCCGACCTGATCC
GCCGCAATGAGCTGGGCGAGCTCAAGGGCATCATGGAAAAGTCCGAAGAGTTGGGCATGCAGACCTTTGACCAGGCCTTG
TTCAACCTGGTGGCAGAGGGAGCCATCGATGAGGAAGAAGCGCTGAAAAACGCTGATTCGGCGAACAACCTGCGCTTGCG
GCTGAAGTTGCATGCCGAGTCGGGCGCGGCGCCACCCGCCGATCCGGCGGCGGGTGAGTGGGGGTTGATGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (79-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.338	95.687	0.539
pilU	Acinetobacter baylyi ADP1	51.791	97.844	0.507
pilU	Vibrio cholerae strain A1552	53.143	94.34	0.501
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.42	98.922	0.38
pilT	Vibrio cholerae strain A1552	41.141	89.757	0.369
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	89.757	0.369
pilT	Pseudomonas stutzeri DSM 10701	39.535	92.722	0.367
pilT	Pseudomonas aeruginosa PAK	39.244	92.722	0.364
pilT	Acinetobacter baumannii D1279779	40.12	90.027	0.361
pilT	Acinetobacter baumannii strain A118	40.12	90.027	0.361