Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   M2J84_RS02710 Genome accession   NZ_CP096918
Coordinates   574605..575828 (-) Length   407 a.a.
NCBI ID   WP_045267485.1    Uniprot ID   A0A7X2I2Z9
Organism   Comamonas aquatica strain NY8661     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 569605..580828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2J84_RS02680 (M2J84_02685) - 570521..571453 (+) 933 WP_270152478.1 ATP-binding protein -
  M2J84_RS02685 (M2J84_02690) - 571475..571936 (+) 462 WP_270151284.1 NUDIX domain-containing protein -
  M2J84_RS02690 (M2J84_02695) - 572039..572248 (-) 210 WP_045267488.1 DNA gyrase inhibitor YacG -
  M2J84_RS02695 (M2J84_02700) zapD 572275..573030 (-) 756 WP_042419933.1 cell division protein ZapD -
  M2J84_RS02700 (M2J84_02705) coaE 573125..573733 (-) 609 WP_154163335.1 dephospho-CoA kinase -
  M2J84_RS02705 (M2J84_02710) pilD 573736..574605 (-) 870 WP_270151289.1 prepilin peptidase Machinery gene
  M2J84_RS02710 (M2J84_02715) pilC 574605..575828 (-) 1224 WP_045267485.1 type II secretion system F family protein Machinery gene
  M2J84_RS02715 (M2J84_02720) pilB 575880..577625 (-) 1746 WP_270151291.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M2J84_RS02720 (M2J84_02725) - 577784..578713 (-) 930 WP_270152480.1 polyprenyl synthetase family protein -
  M2J84_RS02725 (M2J84_02730) rplU 578924..579235 (+) 312 WP_042417032.1 50S ribosomal protein L21 -
  M2J84_RS02730 (M2J84_02735) rpmA 579254..579514 (+) 261 WP_042416998.1 50S ribosomal protein L27 -
  M2J84_RS02735 (M2J84_02740) cgtA 579678..580751 (+) 1074 WP_154163305.1 Obg family GTPase CgtA -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44589.73 Da        Isoelectric Point: 9.5471

>NTDB_id=685148 M2J84_RS02710 WP_045267485.1 574605..575828(-) (pilC) [Comamonas aquatica strain NY8661]
MATAAPGRSNAKEFIYEWAGKDRQGKALHGEMRAVGEAQVQASLRRQGIFITKLKKRSMRAGKKIKPKDIALFTRQMATM
MKAGVPLLQSFDIVGRGNPNPSVTKLLNDIRQDVETGTSLSMAFRKHPMHFNALYCNLIEAGEAAGILDTLMDRLATYME
KTEAIKGKIKSALMYPIAVIVVAFVVVTVIMIFVIPAFKEVFTSFGADLPAPTLLVMAISEFFVAYWWLIFGVIGGGLYF
FMQAWRRSPKMQRVMDRLLLKLPIFGILIDKSCVARWTRTLSTMFAAGVPLVEALDSVGGASGNSVYREATDKIQQMVAT
GTNLTDAMTSADVFPSMVLQMCAIGEESGSIDHMLGKAADFYEQEVDEMVAGLSSLMEPIIIVILGGIIGGIVVSMYLPI
FKLGQVV

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=685148 M2J84_RS02710 WP_045267485.1 574605..575828(-) (pilC) [Comamonas aquatica strain NY8661]
ATGGCAACCGCAGCACCTGGCAGGAGCAACGCAAAGGAGTTCATTTACGAATGGGCCGGCAAAGACCGCCAGGGCAAGGC
ACTGCACGGAGAGATGCGGGCCGTTGGGGAAGCCCAGGTCCAGGCCTCCTTGCGCCGCCAGGGCATCTTCATCACCAAGC
TGAAGAAGCGCAGCATGCGCGCCGGCAAGAAGATCAAGCCCAAGGACATTGCGCTGTTCACCCGCCAGATGGCCACCATG
ATGAAGGCCGGGGTCCCGCTGCTGCAGTCGTTCGACATCGTCGGGCGTGGCAACCCCAATCCCAGCGTGACCAAGCTGCT
CAACGACATCCGCCAGGATGTGGAGACCGGCACCTCGCTGAGCATGGCGTTCCGCAAGCACCCCATGCACTTCAACGCCT
TGTACTGCAACCTGATCGAGGCCGGGGAAGCTGCGGGCATCCTGGACACCTTGATGGACCGCCTGGCCACCTACATGGAA
AAGACCGAGGCCATCAAGGGCAAGATCAAGTCCGCGCTGATGTACCCCATCGCGGTGATCGTGGTGGCGTTCGTGGTGGT
GACGGTGATCATGATCTTTGTGATCCCGGCGTTCAAGGAAGTCTTCACCTCGTTCGGCGCCGACTTGCCGGCCCCGACGC
TACTGGTCATGGCCATCAGTGAGTTCTTTGTCGCGTATTGGTGGCTGATCTTTGGCGTGATCGGTGGCGGCCTGTATTTC
TTCATGCAGGCCTGGCGGCGCAGTCCCAAGATGCAGCGCGTGATGGACCGGCTGCTGCTGAAGCTGCCCATTTTTGGCAT
CCTGATCGACAAATCCTGCGTCGCCCGCTGGACACGCACCCTCTCCACCATGTTCGCCGCCGGTGTGCCCCTGGTGGAAG
CACTGGACTCCGTCGGCGGCGCCTCGGGCAACAGCGTCTACCGGGAAGCCACCGACAAGATCCAGCAGATGGTTGCCACC
GGCACCAATCTCACCGATGCCATGACAAGTGCCGATGTCTTCCCGTCGATGGTGCTGCAGATGTGTGCCATCGGCGAGGA
ATCGGGCTCCATCGATCACATGCTGGGCAAGGCGGCCGACTTCTACGAACAGGAAGTGGACGAGATGGTCGCTGGCCTTT
CCAGCCTGATGGAGCCCATCATCATCGTGATCCTGGGTGGCATCATCGGCGGGATTGTGGTGTCCATGTACCTGCCGATC
TTCAAGTTAGGACAAGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(73-196)

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7X2I2Z9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.887

98.034

0.538
  pilG Neisseria gonorrhoeae MS11

51.01

97.297

0.496
  pilG Neisseria meningitidis 44/76-A

50.758

97.297

0.494
  pilC Legionella pneumophila strain ERS1305867

50.505

97.297

0.491
  pilC Acinetobacter baylyi ADP1

49.246

97.789

0.482
  pilC Acinetobacter baumannii D1279779

48.622

98.034

0.477
  pilC Vibrio campbellii strain DS40M4

40.049

100

0.405
  pilC Thermus thermophilus HB27

39.089

100

0.4
  pilC Vibrio cholerae strain A1552

40.909

97.297

0.398