Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M2J84_RS02715 Genome accession   NZ_CP096918
Coordinates   575880..577625 (-) Length   581 a.a.
NCBI ID   WP_270151291.1    Uniprot ID   -
Organism   Comamonas aquatica strain NY8661     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 570880..582625
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2J84_RS02685 (M2J84_02690) - 571475..571936 (+) 462 WP_270151284.1 NUDIX domain-containing protein -
  M2J84_RS02690 (M2J84_02695) - 572039..572248 (-) 210 WP_045267488.1 DNA gyrase inhibitor YacG -
  M2J84_RS02695 (M2J84_02700) zapD 572275..573030 (-) 756 WP_042419933.1 cell division protein ZapD -
  M2J84_RS02700 (M2J84_02705) coaE 573125..573733 (-) 609 WP_154163335.1 dephospho-CoA kinase -
  M2J84_RS02705 (M2J84_02710) pilD 573736..574605 (-) 870 WP_270151289.1 prepilin peptidase Machinery gene
  M2J84_RS02710 (M2J84_02715) pilC 574605..575828 (-) 1224 WP_045267485.1 type II secretion system F family protein Machinery gene
  M2J84_RS02715 (M2J84_02720) pilB 575880..577625 (-) 1746 WP_270151291.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M2J84_RS02720 (M2J84_02725) - 577784..578713 (-) 930 WP_270152480.1 polyprenyl synthetase family protein -
  M2J84_RS02725 (M2J84_02730) rplU 578924..579235 (+) 312 WP_042417032.1 50S ribosomal protein L21 -
  M2J84_RS02730 (M2J84_02735) rpmA 579254..579514 (+) 261 WP_042416998.1 50S ribosomal protein L27 -
  M2J84_RS02735 (M2J84_02740) cgtA 579678..580751 (+) 1074 WP_154163305.1 Obg family GTPase CgtA -
  M2J84_RS02740 (M2J84_02745) proB 580832..581968 (+) 1137 WP_154163304.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63313.63 Da        Isoelectric Point: 6.3015

>NTDB_id=685149 M2J84_RS02715 WP_270151291.1 575880..577625(-) (pilB) [Comamonas aquatica strain NY8661]
MNAASTASLIAQSSSNTALAGLARALVSAGQLPEAVALETARKAHASKNSFVAQLVASGHMPAAELAFQIAQIFRTPLLD
LDTIDPQRLPRDLLDAKLCSMHKVLPLLKRGNRLVVATADPTDKDAAEKIKFTAQMGVDWVIVECDKLQRLVETTTKSAS
EALDAFNSDADFQFDEVVDAPAPDDKDSNASEVEDAPVVKFLHKMLIDAFNMRASDLHFEPYEHSYRVRFRIDGELREIA
SPPIAIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGADRVIDFRVSTLPTLFGEKIVIRILDPSSARMGIDALGYEPE
EKGRLLDAIQRPYGMILVTGPTGSGKTVSLYSCLNLLNKPGVNISTAEDPSEINMPGVNQVNVNEKAGLTFATALRSFLR
QDPDVIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKQPH
DVPHETLVEAGYEEHQLNGSWVPYKAVGCSACNNGYKGRVGIYQVMPITEAIQQIILRDGSALEIAAQARSEGVKSLRES
GLDKVRQGITSLEEVLAVTNL

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=685149 M2J84_RS02715 WP_270151291.1 575880..577625(-) (pilB) [Comamonas aquatica strain NY8661]
ATGAACGCCGCATCCACTGCATCCTTGATCGCCCAGTCCTCCTCGAACACGGCACTGGCCGGGCTGGCCCGGGCCCTGGT
CAGCGCAGGCCAGCTCCCGGAAGCGGTTGCGCTGGAGACGGCCCGCAAGGCACACGCCAGCAAGAACAGCTTCGTGGCAC
AGCTGGTGGCATCCGGGCACATGCCTGCGGCGGAGCTGGCCTTTCAGATCGCACAGATCTTTCGTACACCGCTGCTGGAC
CTGGACACCATCGATCCCCAGCGCCTGCCCAGGGATTTACTGGACGCCAAGCTGTGCAGCATGCACAAGGTGCTGCCCCT
GCTCAAGCGCGGCAACCGCCTGGTGGTAGCCACAGCAGACCCCACCGACAAGGACGCCGCGGAGAAGATCAAATTCACCG
CCCAGATGGGGGTGGACTGGGTCATCGTCGAATGCGACAAACTGCAGCGGCTGGTCGAAACAACGACCAAGAGTGCCTCT
GAGGCCCTGGACGCCTTCAATTCGGATGCCGACTTCCAGTTCGACGAAGTGGTGGATGCGCCCGCCCCCGACGACAAGGA
CAGCAATGCATCCGAGGTCGAGGACGCGCCGGTCGTCAAGTTCCTGCACAAGATGCTGATTGACGCCTTCAACATGCGGG
CCTCGGACCTGCACTTTGAGCCCTACGAACACAGCTACCGCGTGCGCTTCCGGATCGATGGCGAACTGCGGGAGATCGCG
AGCCCGCCCATCGCCATCAAGGAAAAACTGGCCTCGCGCATCAAGGTGATCTCGCGACTGGACATCTCGGAGAAACGCGT
CCCCCAGGATGGGCGCATGAAGCTCAAGGTCGGTGCTGACCGCGTCATCGACTTCCGTGTGTCCACCTTGCCCACGCTGT
TCGGCGAGAAGATCGTGATCCGTATCCTGGACCCCAGCAGCGCCAGGATGGGCATCGATGCCCTGGGCTACGAGCCCGAG
GAAAAGGGTCGTCTGCTGGATGCCATCCAGCGGCCCTACGGCATGATCCTGGTCACAGGGCCCACCGGCTCGGGCAAAAC
CGTGTCGCTATACAGCTGCCTGAACCTGCTGAACAAACCCGGGGTCAACATCTCCACGGCCGAAGATCCCTCGGAAATCA
ACATGCCCGGCGTCAACCAGGTCAATGTGAATGAAAAGGCCGGACTGACTTTCGCCACCGCCCTGCGATCCTTCCTGCGC
CAGGACCCGGATGTCATCATGGTCGGTGAAATCCGTGATCTGGAAACAGCCGACATCGCCATCAAGGCCGCGCAGACGGG
GCACTTGGTGCTATCCACCCTGCACACCAACGATGCGCCCACCACGTTGACGCGCATGCGCAACATGGGCATTGCGCCGT
TCAACATCGCCTCCAGCGTGATCCTGATCACGGCCCAGCGTCTGGCGCGCCGCTTGTGCCCACAATGCAAGCAGCCGCAT
GATGTGCCACACGAAACCCTGGTCGAAGCCGGTTACGAGGAACACCAGCTGAATGGCTCCTGGGTCCCCTACAAGGCTGT
GGGCTGCAGTGCCTGCAACAATGGTTACAAGGGCCGCGTGGGCATCTACCAGGTGATGCCGATCACCGAAGCAATCCAAC
AAATCATCCTGCGCGACGGCAGTGCCCTGGAGATTGCCGCCCAGGCCAGGAGCGAAGGCGTGAAATCGCTGCGCGAATCC
GGATTGGACAAAGTCCGGCAAGGCATCACTTCGCTGGAAGAGGTGCTGGCCGTCACCAATTTGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-154)

Type II/IV secretion system protein (T2SSE)

(204-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.3

97.418

0.539
  pilB Acinetobacter baylyi ADP1

55.496

97.074

0.539
  pilF Neisseria gonorrhoeae MS11

51.865

96.902

0.503
  pilB Legionella pneumophila strain ERS1305867

50.436

98.623

0.497
  pilB Vibrio cholerae strain A1552

47.197

95.181

0.449
  pilB Vibrio campbellii strain DS40M4

45.633

96.558

0.441
  pilB Vibrio parahaemolyticus RIMD 2210633

46.927

92.427

0.434
  pilF Thermus thermophilus HB27

41.084

98.451

0.404
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.587

91.738

0.363