Detailed information NTG0685147

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M2J84_RS02705
• Genome accession: NZ_CP096918
• Coordinates: 573736..574605 (-)
• Length: 289 a.a.
• NCBI ID: WP_270151289.1
• Uniprot ID: -
• Organism: Comamonas aquatica strain NY8661
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 568736..579605

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M2J84_RS02675 (M2J84_02680)	argJ	569012..570241 (+)	1230	WP_270151282.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
M2J84_RS02680 (M2J84_02685)	-	570521..571453 (+)	933	WP_270152478.1	ATP-binding protein	-
M2J84_RS02685 (M2J84_02690)	-	571475..571936 (+)	462	WP_270151284.1	NUDIX domain-containing protein	-
M2J84_RS02690 (M2J84_02695)	-	572039..572248 (-)	210	WP_045267488.1	DNA gyrase inhibitor YacG	-
M2J84_RS02695 (M2J84_02700)	zapD	572275..573030 (-)	756	WP_042419933.1	cell division protein ZapD	-
M2J84_RS02700 (M2J84_02705)	coaE	573125..573733 (-)	609	WP_154163335.1	dephospho-CoA kinase	-
M2J84_RS02705 (M2J84_02710)	pilD	573736..574605 (-)	870	WP_270151289.1	prepilin peptidase	Machinery gene
M2J84_RS02710 (M2J84_02715)	pilC	574605..575828 (-)	1224	WP_045267485.1	type II secretion system F family protein	Machinery gene
M2J84_RS02715 (M2J84_02720)	pilB	575880..577625 (-)	1746	WP_270151291.1	type IV-A pilus assembly ATPase PilB	Machinery gene
M2J84_RS02720 (M2J84_02725)	-	577784..578713 (-)	930	WP_270152480.1	polyprenyl synthetase family protein	-
M2J84_RS02725 (M2J84_02730)	rplU	578924..579235 (+)	312	WP_042417032.1	50S ribosomal protein L21	-
M2J84_RS02730 (M2J84_02735)	rpmA	579254..579514 (+)	261	WP_042416998.1	50S ribosomal protein L27	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 31441.00 Da
• Isoelectric Point: 7.6447

>NTDB_id=685147 M2J84_RS02705 WP_270151289.1 573736..574605(-) (pilD) [Comamonas aquatica strain NY8661]
MDFSVWSGTALAALLGLLIGSFLNVVIHRLPRMMEQQWDAECAQHRAAQAGQTYTPTPPALTLSKPRSRCPHCAHPIGWL
ENIPVLSWLYLRGQCAHCHAPIGIRYPLVEVATAGLFAWCITHWGFTPTGWAWCGFSATLLALALIDWDTTYLPDDLTLP
LLWAGLLATALGWTSMALGDAVWGAAIGYLSLWSIYWVFKLITGKEGMGYGDFKLFAALGAWFGWQALVPMILMASVIGA
VIGIALKLRSSLREGGYIPFGPFLAGAGFTAMFFGPDRLLRAVLTALGL

Nucleotide

• Length: 870 bp

>NTDB_id=685147 M2J84_RS02705 WP_270151289.1 573736..574605(-) (pilD) [Comamonas aquatica strain NY8661]
ATGGACTTTTCTGTATGGAGCGGTACCGCGCTGGCGGCATTGCTGGGGCTGCTGATCGGCAGCTTCTTGAACGTGGTGAT
CCATCGCCTGCCTCGCATGATGGAGCAGCAATGGGATGCCGAATGCGCACAGCACCGGGCAGCACAGGCGGGTCAAACCT
ACACGCCCACTCCGCCCGCACTGACACTGTCCAAGCCCCGCTCGCGCTGCCCCCACTGCGCCCACCCCATAGGCTGGCTG
GAGAACATCCCGGTGCTCAGCTGGCTGTACCTGCGCGGCCAATGCGCACACTGCCACGCTCCGATCGGCATCCGCTACCC
CCTGGTGGAAGTCGCGACGGCCGGGCTGTTCGCCTGGTGCATCACCCACTGGGGATTCACCCCGACAGGCTGGGCCTGGT
GCGGCTTCAGCGCCACCTTGCTGGCACTGGCGCTGATCGACTGGGACACCACCTACCTGCCCGATGACCTGACCCTGCCG
CTGCTGTGGGCCGGCCTGCTGGCCACTGCGCTGGGCTGGACCTCGATGGCCCTGGGCGACGCCGTCTGGGGCGCCGCCAT
CGGCTACCTGTCGCTGTGGTCCATCTACTGGGTTTTCAAACTGATCACCGGCAAGGAAGGCATGGGCTACGGTGACTTCA
AGCTGTTCGCCGCCCTGGGCGCCTGGTTCGGCTGGCAAGCCCTGGTGCCCATGATCTTGATGGCGTCGGTGATCGGCGCC
GTCATTGGCATCGCCCTGAAGCTGCGCAGCAGCCTGCGCGAGGGGGGCTACATCCCGTTCGGCCCCTTTCTGGCGGGCGC
GGGCTTTACCGCGATGTTCTTCGGCCCAGACCGCCTGCTGCGCGCCGTGCTGACGGCGTTGGGCCTGTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (14-123)

• Type IV leader peptidase family (Peptidase_A24) (138-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.763	96.54	0.519
pilD	Vibrio campbellii strain DS40M4	49.097	95.848	0.471
pilD	Acinetobacter nosocomialis M2	47.872	97.578	0.467
pilD	Acinetobacter baumannii D1279779	47.518	97.578	0.464
pilD	Neisseria gonorrhoeae MS11	45.329	100	0.453