Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   M1B70_RS03850 Genome accession   NZ_CP096571
Coordinates   772901..773326 (+) Length   141 a.a.
NCBI ID   WP_128211868.1    Uniprot ID   -
Organism   Pediococcus acidilactici strain BB2-4M     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 763884..802578 772901..773326 within 0


Gene organization within MGE regions


Location: 763884..802578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1B70_RS03770 (M1B70_03770) - 763884..764999 (-) 1116 WP_128212073.1 site-specific integrase -
  M1B70_RS03775 (M1B70_03775) - 765223..765420 (-) 198 WP_053905909.1 hypothetical protein -
  M1B70_RS03780 (M1B70_03780) - 765567..766694 (-) 1128 WP_159215548.1 hypothetical protein -
  M1B70_RS03785 (M1B70_03785) - 766757..767164 (-) 408 WP_128212081.1 ImmA/IrrE family metallo-endopeptidase -
  M1B70_RS03790 (M1B70_03790) - 767173..767514 (-) 342 WP_002831842.1 helix-turn-helix transcriptional regulator -
  M1B70_RS03795 (M1B70_03795) - 767771..767977 (+) 207 WP_159215549.1 hypothetical protein -
  M1B70_RS03800 (M1B70_03800) - 767967..768275 (-) 309 WP_128211981.1 hypothetical protein -
  M1B70_RS03805 (M1B70_03805) - 768334..768552 (+) 219 WP_235016628.1 helix-turn-helix transcriptional regulator -
  M1B70_RS03810 (M1B70_03810) - 768566..768703 (+) 138 WP_159215550.1 hypothetical protein -
  M1B70_RS03815 (M1B70_03815) - 768695..768889 (-) 195 WP_128211983.1 hypothetical protein -
  M1B70_RS03820 (M1B70_03820) - 768995..769777 (+) 783 WP_128211989.1 phage antirepressor -
  M1B70_RS03825 (M1B70_03825) - 770056..770403 (+) 348 WP_229092966.1 DUF771 domain-containing protein -
  M1B70_RS03830 (M1B70_03830) - 770504..770758 (+) 255 WP_128211987.1 hypothetical protein -
  M1B70_RS03835 (M1B70_03835) - 770868..771530 (-) 663 WP_159215551.1 hypothetical protein -
  M1B70_RS03840 (M1B70_03840) - 771754..772209 (+) 456 WP_128211872.1 chromosome segregation protein SMC -
  M1B70_RS03845 (M1B70_03845) - 772210..772908 (+) 699 WP_128211870.1 ERF family protein -
  M1B70_RS03850 (M1B70_03850) ssb 772901..773326 (+) 426 WP_128211868.1 single-stranded DNA-binding protein Machinery gene
  M1B70_RS03855 (M1B70_03855) - 773338..774021 (+) 684 WP_128211866.1 putative HNHc nuclease -
  M1B70_RS03860 (M1B70_03860) - 774005..774235 (+) 231 WP_128211864.1 hypothetical protein -
  M1B70_RS03865 (M1B70_03865) - 774274..775026 (+) 753 WP_128211862.1 conserved phage C-terminal domain-containing protein -
  M1B70_RS03870 (M1B70_03870) - 775178..775822 (+) 645 WP_229092964.1 ATP-binding protein -
  M1B70_RS03875 (M1B70_03875) - 775940..776572 (+) 633 WP_128211858.1 RNA polymerase subunit sigma-70 -
  M1B70_RS03880 (M1B70_03880) - 776553..776954 (+) 402 WP_128211856.1 RusA family crossover junction endodeoxyribonuclease -
  M1B70_RS03885 (M1B70_03885) - 776951..777100 (+) 150 WP_164873659.1 hypothetical protein -
  M1B70_RS03890 (M1B70_03890) - 777087..777458 (+) 372 WP_128211854.1 N-acetylmuramoyl-L-alanine amidase -
  M1B70_RS03895 (M1B70_03895) - 777461..777601 (+) 141 WP_159215552.1 hypothetical protein -
  M1B70_RS03900 (M1B70_03900) - 777601..777801 (+) 201 WP_128211852.1 hypothetical protein -
  M1B70_RS03905 (M1B70_03905) - 777818..778183 (+) 366 WP_159215553.1 hypothetical protein -
  M1B70_RS03910 (M1B70_03910) - 778187..778495 (+) 309 WP_200834124.1 hypothetical protein -
  M1B70_RS03915 (M1B70_03915) - 778509..778772 (+) 264 WP_128211995.1 hypothetical protein -
  M1B70_RS03920 (M1B70_03920) - 779109..779543 (+) 435 WP_128211997.1 ArpU family phage packaging/lysis transcriptional regulator -
  M1B70_RS03925 (M1B70_03925) - 779765..780085 (+) 321 WP_159215554.1 bacteriocin immunity protein -
  M1B70_RS03930 (M1B70_03930) - 780170..780625 (+) 456 WP_128212001.1 HNH endonuclease signature motif containing protein -
  M1B70_RS03935 (M1B70_03935) - 780622..780921 (+) 300 WP_128212003.1 hypothetical protein -
  M1B70_RS03940 (M1B70_03940) - 781120..781584 (+) 465 WP_128211816.1 phage terminase small subunit P27 family -
  M1B70_RS03945 (M1B70_03945) - 781584..783458 (+) 1875 WP_159215555.1 terminase TerL endonuclease subunit -
  M1B70_RS03950 (M1B70_03950) - 783659..784771 (+) 1113 WP_128211812.1 phage portal protein -
  M1B70_RS03955 (M1B70_03955) - 784752..785345 (+) 594 WP_230312667.1 HK97 family phage prohead protease -
  M1B70_RS03960 (M1B70_03960) - 785346..786680 (+) 1335 WP_230312668.1 phage major capsid protein -
  M1B70_RS03965 (M1B70_03965) - 786763..787233 (+) 471 WP_128211810.1 Ig-like domain-containing protein -
  M1B70_RS03970 (M1B70_03970) - 787249..787572 (+) 324 WP_128211808.1 head-tail connector protein -
  M1B70_RS03975 (M1B70_03975) - 787562..787909 (+) 348 WP_229092888.1 phage head closure protein -
  M1B70_RS03980 (M1B70_03980) - 787909..788322 (+) 414 WP_128211804.1 HK97 gp10 family phage protein -
  M1B70_RS03985 (M1B70_03985) - 788325..788699 (+) 375 WP_159215557.1 DUF806 family protein -
  M1B70_RS03990 (M1B70_03990) - 788712..789314 (+) 603 WP_159215558.1 phage tail protein -
  M1B70_RS03995 (M1B70_03995) - 789451..789855 (+) 405 WP_128211654.1 phage tail tube assembly chaperone -
  M1B70_RS04000 (M1B70_04000) - 789888..790058 (+) 171 WP_164873654.1 hypothetical protein -
  M1B70_RS04005 (M1B70_04005) - 790062..795176 (+) 5115 WP_128211656.1 tape measure protein -
  M1B70_RS04010 (M1B70_04010) - 795189..796022 (+) 834 WP_128211658.1 phage tail domain-containing protein -
  M1B70_RS04015 (M1B70_04015) - 796081..797166 (+) 1086 WP_159215560.1 phage tail protein -
  M1B70_RS04020 (M1B70_04020) - 797156..797476 (+) 321 WP_128211663.1 hypothetical protein -
  M1B70_RS04025 (M1B70_04025) - 797477..797788 (+) 312 WP_128211665.1 hypothetical protein -
  M1B70_RS04030 (M1B70_04030) - 797788..799521 (+) 1734 WP_159215561.1 BppU family phage baseplate upper protein -
  M1B70_RS04035 (M1B70_04035) - 799514..800473 (+) 960 WP_128211669.1 hypothetical protein -
  M1B70_RS04040 (M1B70_04040) - 800473..800751 (+) 279 WP_128211671.1 hypothetical protein -
  M1B70_RS04045 (M1B70_04045) - 800751..800897 (+) 147 WP_075139822.1 XkdX family protein -
  M1B70_RS04050 (M1B70_04050) - 801004..801231 (+) 228 WP_235020341.1 hypothetical protein -
  M1B70_RS04055 (M1B70_04055) - 801231..801473 (+) 243 WP_128211673.1 phage holin -
  M1B70_RS04060 (M1B70_04060) - 801457..802578 (+) 1122 WP_128211675.1 peptidoglycan recognition family protein -

Sequence


Protein


Download         Length: 141 a.a.        Molecular weight: 15657.41 Da        Isoelectric Point: 7.9971

>NTDB_id=681798 M1B70_RS03850 WP_128211868.1 772901..773326(+) (ssb) [Pediococcus acidilactici strain BB2-4M]
MINRTVLIGRLTKDVELRHTAKGDAVASFTVAVNRQFTNSQGEREADFINCVMWRKAAENFAKYTQKGSLVGIDGRIQTR
SYENQQGQRVYVTEVVADNFSLLDSKPKGNQQNNARQASTPGDLFANGGQSIDISDDQLPF

Nucleotide


Download         Length: 426 bp        

>NTDB_id=681798 M1B70_RS03850 WP_128211868.1 772901..773326(+) (ssb) [Pediococcus acidilactici strain BB2-4M]
ATGATTAATCGAACAGTCCTAATAGGACGCCTAACTAAAGATGTTGAACTTCGCCACACAGCTAAAGGTGATGCGGTAGC
TAGTTTTACCGTGGCAGTTAACCGACAGTTTACCAACTCACAGGGTGAACGCGAAGCGGATTTCATCAACTGTGTAATGT
GGCGTAAGGCAGCAGAAAACTTTGCCAAGTACACACAAAAAGGTTCGTTGGTAGGCATTGACGGTCGAATTCAAACCCGT
TCGTACGAAAACCAACAAGGACAACGAGTTTATGTAACTGAGGTTGTAGCTGATAACTTCTCGTTGCTAGATTCGAAACC
AAAAGGCAACCAGCAAAATAATGCACGGCAAGCATCAACGCCGGGAGATCTGTTCGCCAATGGTGGTCAATCAATCGACA
TTTCAGATGATCAGCTCCCCTTTTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

58.824

100

0.709

  ssbA Bacillus subtilis subsp. subtilis str. 168

54.07

100

0.66

  ssbB Bacillus subtilis subsp. subtilis str. 168

62.037

76.596

0.475

  ssbB Streptococcus sobrinus strain NIDR 6715-7

43.662

100

0.44

  ssbB/cilA Streptococcus pneumoniae TIGR4

42.958

100

0.433

  ssbB/cilA Streptococcus mitis NCTC 12261

42.958

100

0.433

  ssbB/cilA Streptococcus pneumoniae Rx1

42.254

100

0.426

  ssbB/cilA Streptococcus pneumoniae D39

42.254

100

0.426

  ssbB/cilA Streptococcus pneumoniae R6

42.254

100

0.426

  ssbB/cilA Streptococcus mitis SK321

42.254

100

0.426

  ssbA Streptococcus mutans UA159

39.007

100

0.39