Detailed information NTG0677797

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LGT42_RS10540
• Genome accession: NZ_CP095733
• Coordinates: 2192551..2193648 (-)
• Length: 365 a.a.
• NCBI ID: WP_226072179.1
• Uniprot ID: -
• Organism: Methylophaga pinxianii strain TMB1265
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2187551..2198648

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LGT42_RS10520 (LGT42_010520)	kdsA	2187620..2188456 (-)	837	WP_226071868.1	3-deoxy-8-phosphooctulonate synthase	-
LGT42_RS10525 (LGT42_010525)	-	2188456..2190093 (-)	1638	WP_226071869.1	CTP synthase	-
LGT42_RS10530 (LGT42_010530)	tilS	2190181..2191524 (-)	1344	WP_226071870.1	tRNA lysidine(34) synthetase TilS	-
LGT42_RS10535 (LGT42_010535)	-	2191534..2192490 (-)	957	WP_226071871.1	acetyl-CoA carboxylase carboxyltransferase subunit alpha	-
LGT42_RS10540 (LGT42_010540)	pilU	2192551..2193648 (-)	1098	WP_226072179.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LGT42_RS10545 (LGT42_010545)	dnaE	2193737..2197222 (-)	3486	WP_226071872.1	DNA polymerase III subunit alpha	-
LGT42_RS10550 (LGT42_010550)	nagZ	2197298..2198347 (-)	1050	WP_226071873.1	beta-N-acetylhexosaminidase	-

Sequence

Protein

• Length: 365 a.a.
• Molecular weight: 40823.96 Da
• Isoelectric Point: 5.4291

>NTDB_id=677797 LGT42_RS10540 WP_226072179.1 2192551..2193648(-) (pilU) [Methylophaga pinxianii strain TMB1265]
MMPYLKLMADKDASDLFFCTAIEPQIKIQGVIRPIGNKKMLTGEVKELAYSLMDQQQIAEFETNLEMNFAVPVHGVGRFR
VNIFRQRGEVSMVIRYIRTHIPNFEEMSLPSVLGNVVMEKRGLILVVGATGSGKSTTLAAMIGHRNRNSGSHILTIEDPL
EFVHSHQRSIVQQREVGIDTLSYENALKNALREAPDVILIGEIRDMDTMKHAINYSETGHLCLATLHANNANQALDRIIN
FFPEMMHRQILMDLSLNLRAIISQRLLPSVEDGKLVPAVEVMLSSPYIAELINKGQISAIKDAMKESSEPGTITFDQALL
ALYREGKISQEDALRNADSRNDLSLEIRMGATHDDNDAEDELILS

Nucleotide

• Length: 1098 bp

>NTDB_id=677797 LGT42_RS10540 WP_226072179.1 2192551..2193648(-) (pilU) [Methylophaga pinxianii strain TMB1265]
ATGATGCCTTACCTGAAACTGATGGCTGATAAAGATGCCTCGGATTTGTTTTTTTGTACCGCTATTGAACCGCAAATCAA
AATTCAGGGTGTTATTCGTCCGATTGGTAACAAAAAAATGCTGACGGGTGAGGTGAAAGAGCTGGCTTATTCACTGATGG
ACCAGCAGCAGATTGCCGAGTTTGAAACCAATTTGGAAATGAACTTTGCTGTGCCCGTGCATGGTGTGGGCCGGTTCAGG
GTAAACATCTTCCGACAACGCGGTGAAGTGTCGATGGTGATTCGATACATCCGTACTCATATCCCTAATTTTGAAGAAAT
GAGTTTACCCTCGGTATTGGGTAATGTGGTGATGGAAAAGCGTGGCCTGATTCTGGTTGTCGGCGCTACGGGCAGCGGTA
AATCCACTACCTTAGCTGCGATGATAGGTCACCGTAACCGTAATTCCGGTTCGCATATTCTGACCATTGAAGATCCGCTG
GAATTTGTGCATTCGCATCAACGTTCTATCGTGCAGCAGCGTGAAGTAGGCATTGATACCCTGTCATATGAAAATGCCTT
GAAAAATGCATTGCGTGAAGCACCGGACGTTATCCTTATCGGTGAAATCCGCGATATGGACACCATGAAACATGCAATAA
ACTACTCAGAAACCGGTCATCTTTGTTTGGCGACATTGCATGCCAATAATGCCAATCAGGCATTGGATCGCATTATTAAC
TTTTTCCCCGAAATGATGCATCGCCAGATCCTCATGGACTTATCACTAAATTTGCGCGCGATTATTTCGCAGCGCTTATT
ACCCTCAGTAGAGGATGGCAAACTGGTACCGGCAGTGGAAGTGATGCTCAGCTCTCCCTATATCGCGGAATTGATCAATA
AAGGTCAAATAAGTGCGATTAAAGATGCGATGAAAGAAAGCAGCGAACCTGGCACCATTACCTTTGACCAGGCTTTGTTA
GCGCTCTATCGTGAAGGCAAGATTTCTCAGGAAGATGCGCTACGCAATGCGGATTCCCGTAATGACCTCAGCCTGGAAAT
CCGGATGGGGGCAACCCATGATGATAATGATGCAGAAGATGAATTAATCCTCAGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (53-267)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.556	96.164	0.534
pilU	Vibrio cholerae strain A1552	53.179	94.795	0.504
pilU	Acinetobacter baylyi ADP1	51.297	95.068	0.488
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.544	91.233	0.397
pilT	Neisseria gonorrhoeae MS11	39.883	93.425	0.373
pilT	Neisseria meningitidis 8013	39.883	93.425	0.373
pilT	Pseudomonas aeruginosa PAK	40.24	91.233	0.367
pilT	Pseudomonas stutzeri DSM 10701	40.24	91.233	0.367
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.244	89.863	0.362
pilT	Vibrio cholerae strain A1552	40.244	89.863	0.362