Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   MU539_RS03810 Genome accession   NZ_CP095384
Coordinates   787364..787801 (+) Length   145 a.a.
NCBI ID   WP_257586235.1    Uniprot ID   -
Organism   Lacticaseibacillus rhamnosus strain VHProbi M14     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 779892..818897 787364..787801 within 0


Gene organization within MGE regions


Location: 779892..818897
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MU539_RS03740 (MU539_03730) - 779892..781019 (-) 1128 WP_257586224.1 site-specific integrase -
  MU539_RS03745 (MU539_03735) - 781183..782028 (-) 846 WP_257586225.1 SHOCT domain-containing protein -
  MU539_RS03750 (MU539_03740) - 782088..782861 (-) 774 WP_257586227.1 XRE family transcriptional regulator -
  MU539_RS03755 (MU539_03745) - 783019..783270 (+) 252 WP_005714763.1 helix-turn-helix transcriptional regulator -
  MU539_RS03760 (MU539_03750) - 783267..783482 (+) 216 WP_064520385.1 BRO family protein -
  MU539_RS03765 (MU539_03755) - 783507..784250 (+) 744 WP_064521148.1 phage antirepressor KilAC domain-containing protein -
  MU539_RS03770 (MU539_03760) - 784247..784396 (+) 150 WP_005712705.1 hypothetical protein -
  MU539_RS03775 (MU539_03765) - 784393..784593 (-) 201 WP_003564805.1 hypothetical protein -
  MU539_RS03780 (MU539_03770) - 784668..785024 (+) 357 WP_257586230.1 DUF771 domain-containing protein -
  MU539_RS03785 (MU539_03775) - 785109..785261 (+) 153 WP_164883155.1 hypothetical protein -
  MU539_RS03790 (MU539_03780) - 785266..785469 (+) 204 WP_257586231.1 hypothetical protein -
  MU539_RS03795 (MU539_03785) - 785487..785978 (+) 492 WP_061713682.1 siphovirus Gp157 family protein -
  MU539_RS03800 (MU539_03790) - 785990..786706 (+) 717 WP_257586233.1 ERF family protein -
  MU539_RS03805 (MU539_03795) - 786681..787349 (+) 669 WP_257586234.1 putative HNHc nuclease -
  MU539_RS03810 (MU539_03800) ssb 787364..787801 (+) 438 WP_257586235.1 single-stranded DNA-binding protein Machinery gene
  MU539_RS03815 (MU539_03805) - 787818..788645 (+) 828 WP_257586236.1 helix-turn-helix domain-containing protein -
  MU539_RS03820 (MU539_03810) - 788642..789904 (+) 1263 WP_257586237.1 DnaB helicase C-terminal domain-containing protein -
  MU539_RS03825 (MU539_03815) - 789906..790250 (+) 345 WP_070792325.1 hypothetical protein -
  MU539_RS03830 (MU539_03820) - 790263..790550 (+) 288 WP_070792327.1 helix-turn-helix transcriptional regulator -
  MU539_RS03835 (MU539_03825) - 790537..790791 (+) 255 WP_257586239.1 hypothetical protein -
  MU539_RS03840 (MU539_03830) - 790788..791153 (+) 366 WP_003607027.1 endodeoxyribonuclease -
  MU539_RS03845 (MU539_03835) - 791166..791630 (+) 465 WP_015764333.1 hypothetical protein -
  MU539_RS03850 (MU539_03840) - 791642..791827 (+) 186 WP_014569476.1 hypothetical protein -
  MU539_RS03855 (MU539_03845) - 791824..792372 (+) 549 WP_047676133.1 DUF1642 domain-containing protein -
  MU539_RS03860 (MU539_03850) - 792362..792538 (+) 177 WP_003582729.1 hypothetical protein -
  MU539_RS03865 (MU539_03855) - 792593..792814 (+) 222 WP_005711376.1 helix-turn-helix domain-containing protein -
  MU539_RS03870 (MU539_03860) - 793020..793448 (+) 429 WP_257586241.1 ArpU family phage packaging/lysis transcriptional regulator -
  MU539_RS03875 (MU539_03865) - 793732..794733 (+) 1002 WP_257586468.1 hypothetical protein -
  MU539_RS03880 (MU539_03870) - 795266..796483 (+) 1218 WP_024129330.1 GcrA family cell cycle regulator -
  MU539_RS03885 (MU539_03875) - 796470..797000 (+) 531 WP_021354318.1 NUMOD4 motif-containing HNH endonuclease -
  MU539_RS13640 - 797004..797090 (+) 87 Protein_739 ribonucleoside-diphosphate reductase -
  MU539_RS03890 (MU539_03880) - 797103..797420 (+) 318 WP_306808353.1 hypothetical protein -
  MU539_RS03895 (MU539_03885) - 797432..797866 (+) 435 Protein_741 hypothetical protein -
  MU539_RS03900 (MU539_03890) - 797894..798049 (+) 156 WP_257586519.1 hypothetical protein -
  MU539_RS03905 (MU539_03895) - 798062..798550 (+) 489 WP_257586244.1 HNH endonuclease -
  MU539_RS03910 (MU539_03900) - 798686..799159 (+) 474 WP_024129329.1 phage terminase small subunit P27 family -
  MU539_RS03915 (MU539_03905) - 799156..801048 (+) 1893 WP_257586246.1 terminase large subunit -
  MU539_RS03920 (MU539_03910) - 801035..801244 (+) 210 WP_080751823.1 hypothetical protein -
  MU539_RS03925 (MU539_03915) - 801244..802431 (+) 1188 WP_039141544.1 phage portal protein -
  MU539_RS03930 (MU539_03920) - 802409..803125 (+) 717 WP_257586247.1 head maturation protease, ClpP-related -
  MU539_RS03935 (MU539_03925) - 803125..804366 (+) 1242 WP_257586248.1 phage major capsid protein -
  MU539_RS03940 (MU539_03930) - 804438..804746 (+) 309 WP_257586249.1 hypothetical protein -
  MU539_RS03945 (MU539_03935) - 804736..805080 (+) 345 WP_257586469.1 phage head closure protein -
  MU539_RS03950 (MU539_03940) - 805083..805502 (+) 420 WP_024129324.1 HK97-gp10 family putative phage morphogenesis protein -
  MU539_RS03955 (MU539_03945) - 805499..805870 (+) 372 WP_021354702.1 DUF806 family protein -
  MU539_RS03960 (MU539_03950) - 805882..806517 (+) 636 WP_257586252.1 phage tail protein -
  MU539_RS03965 (MU539_03955) - 806675..807025 (+) 351 WP_257586253.1 phage tail assembly chaperone -
  MU539_RS03970 (MU539_03960) - 807003..807233 (+) 231 WP_057709200.1 hypothetical protein -
  MU539_RS03975 (MU539_03965) - 807263..811339 (+) 4077 WP_257586254.1 tape measure protein -
  MU539_RS03980 (MU539_03970) - 811340..813289 (+) 1950 WP_257586256.1 distal tail protein Dit -
  MU539_RS03985 (MU539_03975) - 813290..816238 (+) 2949 WP_257586258.1 collagen-like protein -
  MU539_RS03990 (MU539_03980) - 816254..816544 (+) 291 WP_257586259.1 hypothetical protein -
  MU539_RS03995 (MU539_03985) - 816606..816668 (+) 63 WP_225424246.1 hypothetical protein -
  MU539_RS04000 (MU539_03990) - 816649..816999 (+) 351 WP_225350104.1 hypothetical protein -
  MU539_RS04005 (MU539_03995) - 817014..817427 (+) 414 WP_032961125.1 phage holin -
  MU539_RS04010 (MU539_04000) - 817442..817714 (+) 273 WP_032964520.1 DUF6275 family protein -
  MU539_RS04015 (MU539_04005) - 817725..818897 (+) 1173 WP_257586261.1 GH25 family lysozyme -

Sequence


Protein


Download         Length: 145 a.a.        Molecular weight: 15924.54 Da        Isoelectric Point: 5.7222

>NTDB_id=676618 MU539_RS03810 WP_257586235.1 787364..787801(+) (ssb) [Lacticaseibacillus rhamnosus strain VHProbi M14]
MLNSVALTGRLTKDVDLRYTQSGTAVGSFTIAVDRQFRSANGNRETDFINCVIWTKSAENLAKFTHKGSLIGVEGHIQTR
TYDNAQGNKVYVTEVVVENFALLEPRQTSQESQQRSANNPAAASQGNRFANNGQPIDVSDDDLPF

Nucleotide


Download         Length: 438 bp        

>NTDB_id=676618 MU539_RS03810 WP_257586235.1 787364..787801(+) (ssb) [Lacticaseibacillus rhamnosus strain VHProbi M14]
ATGCTTAATTCAGTTGCTTTAACAGGCAGATTAACTAAAGACGTTGACCTTCGCTACACACAAAGCGGAACGGCAGTCGG
TTCATTCACAATCGCTGTTGACCGTCAATTCCGCAGTGCGAACGGTAACCGGGAGACTGACTTCATCAATTGTGTCATCT
GGACTAAGTCAGCTGAGAACCTCGCTAAGTTCACGCATAAGGGTTCACTCATTGGTGTTGAAGGTCACATTCAGACGCGC
ACATACGACAACGCACAAGGCAATAAAGTGTATGTAACTGAGGTCGTTGTCGAGAATTTCGCCTTGCTTGAGCCACGGCA
GACGTCTCAGGAAAGCCAACAACGATCGGCTAATAACCCAGCGGCCGCAAGCCAAGGCAACAGGTTTGCCAACAACGGTC
AGCCAATCGATGTCAGCGATGATGATCTTCCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

57.647

100

0.676

  ssbA Bacillus subtilis subsp. subtilis str. 168

47.093

100

0.559

  ssbB/cilA Streptococcus pneumoniae TIGR4

40.268

100

0.414

  ssbB/cilA Streptococcus mitis NCTC 12261

40.268

100

0.414

  ssbB/cilA Streptococcus pneumoniae Rx1

39.597

100

0.407

  ssbB/cilA Streptococcus pneumoniae D39

39.597

100

0.407

  ssbB/cilA Streptococcus pneumoniae R6

39.597

100

0.407

  ssbB/cilA Streptococcus mitis SK321

39.597

100

0.407

  ssbB Bacillus subtilis subsp. subtilis str. 168

52.83

73.103

0.386

  ssbB Streptococcus sobrinus strain NIDR 6715-7

37.241

100

0.372

  ssb Neisseria gonorrhoeae MS11

31.395

100

0.372