Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MRK42_RS13265 Genome accession   NZ_CP095328
Coordinates   2901673..2902812 (+) Length   379 a.a.
NCBI ID   WP_342010804.1    Uniprot ID   -
Organism   Aeromonas sp. 19NY04SH05-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2896673..2907812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRK42_RS13235 (MRK42_13200) - 2896863..2897243 (-) 381 WP_338610404.1 diacylglycerol kinase -
  MRK42_RS13240 (MRK42_13205) - 2897387..2898448 (+) 1062 WP_026456871.1 3-deoxy-7-phosphoheptulonate synthase -
  MRK42_RS13245 (MRK42_13210) - 2898529..2899314 (-) 786 WP_225631846.1 ATP-binding cassette domain-containing protein -
  MRK42_RS13250 (MRK42_13215) btuC 2899301..2900308 (-) 1008 WP_338610406.1 vitamin B12 ABC transporter permease BtuC -
  MRK42_RS13255 (MRK42_13220) - 2900411..2901142 (-) 732 WP_354688282.1 transporter substrate-binding domain-containing protein -
  MRK42_RS13260 (MRK42_13225) - 2901257..2901613 (+) 357 WP_005337656.1 DMT family protein -
  MRK42_RS13265 (MRK42_13230) pilU 2901673..2902812 (+) 1140 WP_342010804.1 type IVa pilus ATPase TapW Machinery gene
  MRK42_RS13270 (MRK42_13235) - 2902883..2903332 (-) 450 WP_042069911.1 isoprenylcysteine carboxylmethyltransferase family protein -
  MRK42_RS13275 (MRK42_13240) alr 2903496..2904722 (+) 1227 WP_042028580.1 alanine racemase -
  MRK42_RS13280 (MRK42_13245) - 2904785..2905729 (-) 945 WP_042028579.1 TerC family protein -
  MRK42_RS13285 (MRK42_13250) oppF 2906027..2907022 (-) 996 WP_042028578.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42280.32 Da        Isoelectric Point: 6.4287

>NTDB_id=676363 MRK42_RS13265 WP_342010804.1 2901673..2902812(+) (pilU) [Aeromonas sp. 19NY04SH05-1]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGVLRPLSETPLNPGDVASIADAIMDKEQKEQFERELEMNLAISLPQVGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTSDGGRRAAVEIMLGTHTIRDMVKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWREKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=676363 MRK42_RS13265 WP_342010804.1 2901673..2902812(+) (pilU) [Aeromonas sp. 19NY04SH05-1]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTTTATCTGTCAACCGGCGCCCCCCCCTG
CGCCAAGTTCAACGGGGTGTTGCGCCCGCTGAGCGAGACCCCCCTCAATCCGGGAGATGTAGCGAGCATCGCCGACGCCA
TCATGGACAAGGAGCAGAAGGAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGGTAGGCCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAGGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCGCGCTTTGA
GGATCTCAAACTCCCCCCCGTGCTGCTCGACACCATCATGGAAAAACGGGGTCTGGTGCTGTTCGTCGGCGGTACCGGCT
CGGGCAAATCCACCTCGCTGGCGGCCCTTATCGATCACCGCAACCGCAACAGCGGCGGCCACATCATCACCATTGAGGAT
CCGGTGGAGTTCGTTCACCGCCACCGCAAGAGCATCATCAACCAGCGCGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACTCTGCGCCAGGCGCCGGACGTGATCCTCATCGGGGAAATTCGTGATCGCGAGACCATGGAGCATG
CCCTGGCCTTCTCGGAAACCGGTCACCTCGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGACGCCCGCAATTGCTCAACGATCTCGGCAACAACCTCAAGGCATTTGTCTCCCAACG
GCTGGTGAAGACCTCGGACGGCGGTCGCCGGGCGGCGGTGGAGATCATGCTCGGCACTCACACCATACGTGACATGGTCA
AACGGGGTGAATTTGGCGGCCTGAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATCGATGAAGAAGAAGCAGTGAAAAACGCCGACTCGGCCAATAACCTGCGCCTCAA
GATCAAGTTGTGGCGGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAACCCACCAAGGATG
AGAAAGACGACCTCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.6

98.945

0.53
  pilU Acinetobacter baylyi ADP1

52.958

93.668

0.496
  pilU Vibrio cholerae strain A1552

50.142

93.14

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.076

93.14

0.383
  pilT Legionella pneumophila strain ERS1305867

41.617

88.127

0.367
  pilT Legionella pneumophila strain Lp02

41.617

88.127

0.367
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364