Detailed information NTG0676022

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MUU75_RS16050
• Genome accession: NZ_CP095186
• Coordinates: 3434327..3435457 (+)
• Length: 376 a.a.
• NCBI ID: WP_245092139.1
• Uniprot ID: -
• Organism: Pseudoxanthomonas mexicana strain F31
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3429327..3440457

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MUU75_RS16025 (MUU75_16025)	-	3430031..3430837 (+)	807	WP_245106501.1	IS110 family transposase	-
MUU75_RS16030 (MUU75_16030)	-	3430987..3431448 (-)	462	WP_245092125.1	DUF4426 domain-containing protein	-
MUU75_RS16035 (MUU75_16035)	proC	3431509..3432351 (-)	843	WP_245092137.1	pyrroline-5-carboxylate reductase	-
MUU75_RS16040 (MUU75_16040)	-	3432382..3433065 (-)	684	WP_245094236.1	YggS family pyridoxal phosphate-dependent enzyme	-
MUU75_RS16045 (MUU75_16045)	-	3433159..3434195 (+)	1037	Protein_3176	type IV pilus twitching motility protein PilT	-
MUU75_RS16050 (MUU75_16050)	pilU	3434327..3435457 (+)	1131	WP_245092139.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MUU75_RS16055 (MUU75_16055)	-	3435801..3438923 (-)	3123	WP_245092140.1	TonB-dependent receptor	-
MUU75_RS16060 (MUU75_16060)	-	3438922..3439065 (+)	144	WP_245092142.1	hypothetical protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 41891.25 Da
• Isoelectric Point: 6.8287

>NTDB_id=676022 MUU75_RS16050 WP_245092139.1 3434327..3435457(+) (pilU) [Pseudoxanthomonas mexicana strain F31]
MSTIDFTSFLKLMAHQKASDLFITSGMPPSIKVHGKISPITQTPLTAQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTIEELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRTQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIRDGEIHKLKEVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDARTLAQGLDGVEIAEVR

Nucleotide

• Length: 1131 bp

>NTDB_id=676022 MUU75_RS16050 WP_245092139.1 3434327..3435457(+) (pilU) [Pseudoxanthomonas mexicana strain F31]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACGTCCGGCAT
GCCGCCGTCCATCAAGGTGCACGGCAAGATTTCGCCGATCACCCAGACTCCGCTGACGGCGCAGCAGTCGCGCGACCTGG
TGCTGAACGTGATGACGCCGGCGCAGCGCGAGGAATTCGAAAAGACCCACGAATGCAACTTCGCCATCGGCGTGGCCGGC
GTGGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGCCGCATCGAGACGCGCATCCC
GACCATCGAAGAGCTGAACCTGCCGCCGGTCATCAAGACGCTGGCCATGACCAAGCGCGGCATCATCATCTTCGTGGGCG
CCACCGGCACCGGCAAGTCCACGTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGCCACATCATCACC
ATCGAAGACCCCATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGTGAAGTCGGCATCGACACCGACAG
CTGGGAAGCGGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCACCCAGCTGCTGATGGACCTGTCGCTCAACCTGAAGGGCGTGGT
GGCGCAGCAGCTGATCCCCACGCCGGACGGCAAGGGGCGCCGCGTGGCGATGGAGATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATGAAGGAGTCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCTATGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAACTGGCCCAGGGTGGCGATGCCCGCACGCTGGCGCAGGGCCTGGATGGCGTGGAGATCGCGG
AAGTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (117-272)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	67.847	97.606	0.662
pilU	Acinetobacter baylyi ADP1	63.866	94.947	0.606
pilU	Vibrio cholerae strain A1552	54.213	94.681	0.513
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.857	93.085	0.399
pilT	Acinetobacter baumannii strain A118	40.407	91.489	0.37
pilT	Acinetobacter nosocomialis M2	40.407	91.489	0.37
pilT	Acinetobacter baumannii D1279779	40.407	91.489	0.37
pilT	Pseudomonas aeruginosa PAK	41.194	89.096	0.367
pilT	Legionella pneumophila strain Lp02	40.597	89.096	0.362
pilT	Legionella pneumophila strain ERS1305867	40.597	89.096	0.362