Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MUU73_RS04665 Genome accession   NZ_CP095184
Coordinates   975253..976383 (-) Length   376 a.a.
NCBI ID   WP_162110440.1    Uniprot ID   -
Organism   Pseudoxanthomonas mexicana strain F12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 970253..981383
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUU73_RS04660 (MUU73_04660) - 974539..975072 (-) 534 WP_245125116.1 DUF6622 family protein -
  MUU73_RS04665 (MUU73_04665) pilU 975253..976383 (-) 1131 WP_162110440.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MUU73_RS04670 (MUU73_04670) pilT 976515..977552 (-) 1038 WP_093490243.1 type IV pilus twitching motility protein PilT Machinery gene
  MUU73_RS04675 (MUU73_04675) - 977646..978329 (+) 684 WP_245126742.1 YggS family pyridoxal phosphate-dependent enzyme -
  MUU73_RS04680 (MUU73_04680) proC 978360..979202 (+) 843 WP_245125118.1 pyrroline-5-carboxylate reductase -
  MUU73_RS04685 (MUU73_04685) - 979262..979723 (+) 462 WP_245125121.1 DUF4426 domain-containing protein -
  MUU73_RS04690 (MUU73_04690) - 979891..980685 (-) 795 WP_245125123.1 cobalamin-binding protein -
  MUU73_RS04695 (MUU73_04695) aac(6') 980689..981084 (-) 396 WP_245126744.1 aminoglycoside 6'-N-acetyltransferase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41893.23 Da        Isoelectric Point: 6.8286

>NTDB_id=675977 MUU73_RS04665 WP_162110440.1 975253..976383(-) (pilU) [Pseudoxanthomonas mexicana strain F12]
MSTIDFTSFLKLMAHQKASDLFITSGMPPSIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETRIPTIEELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWDAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRTQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIRDGEIHKLKEVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDARTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=675977 MUU73_RS04665 WP_162110440.1 975253..976383(-) (pilU) [Pseudoxanthomonas mexicana strain F12]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACGTCCGGCAT
GCCGCCGTCCATCAAGGTGCACGGCAAGATCTCGCCGATCACGCAGACGCCGCTGACGGCGCAGCAGTCGCGCGACCTGG
TGCTGAACGTGATGACGCCCTCGCAGCGCGAGGAGTTCGAAAAGACCCACGAATGCAACTTCGCCATCGGCGTGGCCGGC
GTGGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTAGGCATGGTGCTGCGCCGCATCGAGACGCGCATCCC
GACCATCGAAGAGCTGAACCTGCCGCCGGTCATCAAGACGCTGGCCATGACCAAGCGCGGCATCATCATCTTCGTGGGTG
CGACCGGTACCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAACTCGACCGGCCACATCATCACC
ATCGAAGACCCCATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGTGAAGTGGGTATCGACACCGACAG
CTGGGATGCGGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCACCCAGCTGCTGATGGACCTGTCGCTCAACCTGAAGGGCGTGGT
AGCGCAGCAGCTGATCCCCACGCCGGACGGCAAGGGCCGCCGCGTGGCGATGGAGATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGTGAGATCCACAAGCTGAAGGAAGTGATGAAGGAGTCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCTACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCCCGCACGCTGGCGCAGGGCCTGGATGGCGTGGAGATCGCGG
AAGTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.143

93.085

0.402
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367
  pilT Legionella pneumophila strain Lp02

40.597

89.096

0.362
  pilT Legionella pneumophila strain ERS1305867

40.597

89.096

0.362