Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ISX51_RS02600 Genome accession   NZ_CP094659
Coordinates   519843..521537 (-) Length   564 a.a.
NCBI ID   WP_281053315.1    Uniprot ID   -
Organism   Vibrio lentus strain ED252     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 514843..526537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISX51_RS02580 (ISX51_02580) - 515519..516367 (-) 849 WP_017108564.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  ISX51_RS02585 (ISX51_02585) - 516437..517954 (-) 1518 WP_281053314.1 AAA family ATPase -
  ISX51_RS02590 (ISX51_02590) aroB 518034..519122 (-) 1089 WP_017104650.1 3-dehydroquinate synthase -
  ISX51_RS02595 (ISX51_02595) aroK 519148..519666 (-) 519 WP_004729664.1 shikimate kinase AroK -
  ISX51_RS02600 (ISX51_02600) pilQ 519843..521537 (-) 1695 WP_281053315.1 type IV pilus secretin PilQ family protein Machinery gene
  ISX51_RS02605 (ISX51_02605) pilP 521597..522115 (-) 519 WP_102279229.1 pilus assembly protein PilP Machinery gene
  ISX51_RS02610 (ISX51_02610) pilO 522102..522713 (-) 612 WP_281053316.1 type 4a pilus biogenesis protein PilO Machinery gene
  ISX51_RS02615 (ISX51_02615) pilN 522706..523320 (-) 615 WP_281053317.1 PilN domain-containing protein Machinery gene
  ISX51_RS02620 (ISX51_02620) pilM 523304..524329 (-) 1026 WP_102485531.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 61369.94 Da        Isoelectric Point: 4.4045

>NTDB_id=670466 ISX51_RS02600 WP_281053315.1 519843..521537(-) (pilQ) [Vibrio lentus strain ED252]
MNKGLQGAVLSMMLFFSVMSHAETSPNNLDNIDFRVNKNKDAVIIIELATSTAVVDVQRVQEGLSIELLNTKVDDDKLYL
LDVKDFATLVEGVEVFRETPSTRLLATIADDYQYEYNLKGRFIEVIVSKPLVKESVAEKSVLEKEGKLISINFQDIPVRN
VLQLIADYNDFNLVVSDSVSGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKAELDLREQQALEKSRLEEELG
ELKSEIIKINFAKATDIADMIGGEGAVSMLSDRGSITIDERTNSLLIRELEENIAVIRGIIESLDIPVKQVQIEARIVTV
TEGNLDELGVRWGVSSTNGSFKVGGSIEGNHPSQIAPYDDNGGDSAIDDYLNVNLGATSPNASSIAFQVAKLGSDTLLDL
ELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLVLDLSVTQDRP
GQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVSSTVDKVPLLGDLPVLGALFRRSYENVGKSELLIFVTPK
VVIQ

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=670466 ISX51_RS02600 WP_281053315.1 519843..521537(-) (pilQ) [Vibrio lentus strain ED252]
ATGAACAAAGGGCTACAGGGTGCAGTGCTGTCTATGATGTTGTTTTTTAGCGTGATGAGTCACGCAGAGACATCACCCAA
TAACTTGGACAACATCGATTTCAGGGTCAACAAGAACAAAGATGCGGTCATCATTATTGAATTGGCAACCAGCACTGCGG
TGGTCGATGTTCAAAGAGTTCAAGAAGGGCTGAGCATTGAACTGCTTAATACTAAGGTGGATGACGACAAGCTTTATTTG
TTGGATGTAAAAGACTTTGCCACCTTAGTTGAAGGGGTTGAAGTCTTTAGAGAAACGCCGAGTACCCGACTGTTAGCCAC
GATCGCCGATGATTATCAATACGAGTACAACCTAAAAGGTCGTTTTATCGAGGTCATCGTGAGTAAGCCGCTGGTTAAAG
AGAGTGTCGCTGAGAAGAGTGTGTTGGAGAAAGAGGGCAAACTCATCTCAATTAACTTCCAAGACATTCCTGTTCGTAAT
GTTCTTCAGCTCATTGCCGATTACAACGACTTCAACTTAGTGGTGTCTGATTCGGTCTCAGGAAACCTCACTTTACGGTT
AGATGGCGTACCTTGGCAGCAAGTTCTCGACATTATCTTACAAGTTAAAGGCTTGGATAAGCGTGTTGATGGCAATGTCA
TCTTGGTTGCGCCGAAAGCTGAACTTGACCTTCGAGAGCAGCAAGCCCTAGAAAAATCTCGTTTGGAAGAAGAACTGGGT
GAGCTTAAATCGGAAATCATCAAGATTAACTTTGCTAAAGCGACCGACATCGCCGATATGATTGGTGGGGAAGGAGCGGT
GAGTATGCTGTCTGATCGTGGCTCGATTACCATTGATGAGAGAACCAATTCACTGCTCATTCGTGAGTTGGAAGAAAACA
TTGCGGTGATACGAGGCATCATTGAATCGCTAGACATTCCCGTGAAGCAAGTTCAGATAGAAGCGCGTATTGTCACTGTT
ACTGAGGGTAATCTTGATGAACTAGGAGTGCGTTGGGGCGTTTCCTCGACCAACGGAAGCTTCAAAGTCGGCGGCTCAAT
AGAAGGTAACCATCCATCACAAATTGCACCGTATGACGATAATGGTGGGGATAGTGCGATTGACGATTACCTCAATGTTA
ATCTAGGGGCGACATCGCCTAATGCATCAAGCATTGCGTTTCAGGTGGCTAAGCTGGGTTCAGATACCTTGCTTGATCTT
GAATTGTCGGCACTACAACAAGAGTCTAAAGCTGAGATTATTTCTAGCCCACGCTTAATCACCACCAATAAAAAGCCCGC
TTACATTGAGCAAGGTACTGAAATCCCTTATTTAGAATCCTCTTCCAGTGGTGCAACTTCGGTCGCATTTAAAAAAGCAG
TCTTGAGTCTTAAGGTCACACCACAGATAACCCCTGATAATCGTCTGGTATTAGATTTGAGTGTTACTCAGGACAGACCT
GGCCAGGTTGTGAAAACAGGAACCGGTGAGGCTGTGGCAATCGACACCCAAAGAATTGGCACGCAAGTGCTTGTTAATAA
TGGTGAAACCGTTGTTCTTGGTGGGATATTTCAACACAGTGTTAGCAGCACAGTCGACAAAGTTCCGTTGTTGGGAGACC
TGCCAGTTTTGGGGGCATTGTTCCGTCGCAGCTATGAAAATGTGGGTAAAAGTGAACTACTTATTTTTGTTACACCTAAA
GTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(171-219)

Bacterial type II/III secretion system short domain (Secretin_N)

(245-310)

Bacterial type II and III secretion system protein (Secretin)

(404-562)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

80.839

97.163

0.785
  pilQ Vibrio cholerae strain A1552

80.256

96.986

0.778
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

80.256

96.986

0.778
  pilQ Legionella pneumophila strain ERS1305867

42.962

96.986

0.417
  pilQ Legionella pneumophila strain Lp02

42.962

96.986

0.417
  pilQ Pseudomonas aeruginosa PAK

40.934

98.759

0.404
  comQ Acinetobacter baylyi ADP1

38.783

100

0.395
  pilQ Acinetobacter baumannii strain A118

37.478

100

0.385
  pilQ Acinetobacter baumannii D1279779

37.478

100

0.385