Detailed information NTG0670470

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ISX51_RS02620
• Genome accession: NZ_CP094659
• Coordinates: 523304..524329 (-)
• Length: 341 a.a.
• NCBI ID: WP_102485531.1
• Uniprot ID: -
• Organism: Vibrio lentus strain ED252
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 518304..529329

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ISX51_RS02595 (ISX51_02595)	aroK	519148..519666 (-)	519	WP_004729664.1	shikimate kinase AroK	-
ISX51_RS02600 (ISX51_02600)	pilQ	519843..521537 (-)	1695	WP_281053315.1	type IV pilus secretin PilQ family protein	Machinery gene
ISX51_RS02605 (ISX51_02605)	pilP	521597..522115 (-)	519	WP_102279229.1	pilus assembly protein PilP	Machinery gene
ISX51_RS02610 (ISX51_02610)	pilO	522102..522713 (-)	612	WP_281053316.1	type 4a pilus biogenesis protein PilO	Machinery gene
ISX51_RS02615 (ISX51_02615)	pilN	522706..523320 (-)	615	WP_281053317.1	PilN domain-containing protein	Machinery gene
ISX51_RS02620 (ISX51_02620)	pilM	523304..524329 (-)	1026	WP_102485531.1	type IV pilus assembly protein PilM	Machinery gene
ISX51_RS02625 (ISX51_02625)	-	524481..527039 (+)	2559	WP_076668779.1	PBP1A family penicillin-binding protein	-
ISX51_RS02630 (ISX51_02630)	oxyR	527153..528046 (-)	894	WP_102485533.1	DNA-binding transcriptional regulator OxyR	-
ISX51_RS02635 (ISX51_02635)	-	528045..528224 (+)	180	WP_281053318.1	hypothetical protein	-
ISX51_RS02640 (ISX51_02640)	-	528268..528996 (+)	729	WP_017073712.1	glutathione peroxidase	-

Sequence

Protein

• Length: 341 a.a.
• Molecular weight: 38164.68 Da
• Isoelectric Point: 6.2417

>NTDB_id=670470 ISX51_RS02620 WP_102485531.1 523304..524329(-) (pilM) [Vibrio lentus strain ED252]
MGSSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKELPFGCRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVESRADALTKAGFKP
VVVDTQAHGLLNIWQLASRMYPEKGNWLLVDVGVDQTSLGIIPQGSAPFYKDIAFGTQDLRSTDTPDDIESVFGTAEETH
KFIVSLIEKLKRQLQLYSSVNALQPISGIWLMGEGASIPMVTEELERHFQLSCESLNPLSLFENKVAKRYRLPMDWQHFG
IAAGMAMSGLKWQGGKHVASN

Nucleotide

• Length: 1026 bp

>NTDB_id=670470 ISX51_RS02620 WP_102485531.1 523304..524329(-) (pilM) [Vibrio lentus strain ED252]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGCATCAAAGCCGTGGTACTAAAACCCGTGGGGGAGTT
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTCTGGAGTATCAGAAAA
CTGTTAAGAAACTTAAAGAACTTAGGAAAGAACTACCTTTTGGCTGTCGTAACGTCGCCATCTCGGTACCTGATAACACA
GTAATCAGTAAAGTACTGCAAATAGAGAGTGAGTTAGAAGACAGAGAAAAAGAGTTTTCTATCTATCAAACCTTTGCTCA
CCAGTCTCCCTTTCCTATCGAGGAGCTGAGTTTAGATTTTGTAAAGCTCGAAGACAAACGATTTGGTAAAGGATCGACAA
GCAGTTATCAGGTGTACGCCACTCGTAAAGAAGTAGTAGAGAGTCGAGCGGATGCTTTAACCAAGGCTGGTTTTAAACCT
GTAGTGGTGGATACGCAGGCGCATGGGTTACTCAATATCTGGCAGTTGGCTTCGCGCATGTATCCAGAAAAGGGTAATTG
GTTGCTGGTGGATGTGGGTGTCGACCAAACATCGTTAGGCATCATTCCACAAGGTTCAGCGCCGTTTTATAAAGATATTG
CCTTTGGTACCCAAGATCTCCGTAGCACTGACACCCCTGATGATATCGAGAGCGTATTCGGCACCGCAGAGGAGACACAT
AAGTTCATTGTTAGCTTGATTGAAAAGCTTAAGCGTCAGTTGCAGCTCTATTCCTCGGTCAATGCGCTTCAACCGATCTC
AGGGATCTGGTTGATGGGTGAGGGCGCCAGCATTCCCATGGTAACCGAAGAGCTTGAGCGTCACTTTCAGCTGAGCTGTG
AGTCGCTAAACCCTTTATCTCTTTTTGAGAATAAGGTCGCCAAGAGATACCGACTGCCGATGGACTGGCAACATTTTGGA
ATTGCGGCTGGTATGGCGATGAGCGGGCTTAAATGGCAAGGAGGAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (242-328)

• Type IV pilus assembly protein PilM; (PilM_2) (8-221)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	54.252	100	0.543
pilM	Vibrio campbellii strain DS40M4	49.56	100	0.496