Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ISX51_RS02615 Genome accession   NZ_CP094659
Coordinates   522706..523320 (-) Length   204 a.a.
NCBI ID   WP_281053317.1    Uniprot ID   -
Organism   Vibrio lentus strain ED252     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 517706..528320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISX51_RS02590 (ISX51_02590) aroB 518034..519122 (-) 1089 WP_017104650.1 3-dehydroquinate synthase -
  ISX51_RS02595 (ISX51_02595) aroK 519148..519666 (-) 519 WP_004729664.1 shikimate kinase AroK -
  ISX51_RS02600 (ISX51_02600) pilQ 519843..521537 (-) 1695 WP_281053315.1 type IV pilus secretin PilQ family protein Machinery gene
  ISX51_RS02605 (ISX51_02605) pilP 521597..522115 (-) 519 WP_102279229.1 pilus assembly protein PilP Machinery gene
  ISX51_RS02610 (ISX51_02610) pilO 522102..522713 (-) 612 WP_281053316.1 type 4a pilus biogenesis protein PilO Machinery gene
  ISX51_RS02615 (ISX51_02615) pilN 522706..523320 (-) 615 WP_281053317.1 PilN domain-containing protein Machinery gene
  ISX51_RS02620 (ISX51_02620) pilM 523304..524329 (-) 1026 WP_102485531.1 type IV pilus assembly protein PilM Machinery gene
  ISX51_RS02625 (ISX51_02625) - 524481..527039 (+) 2559 WP_076668779.1 PBP1A family penicillin-binding protein -
  ISX51_RS02630 (ISX51_02630) oxyR 527153..528046 (-) 894 WP_102485533.1 DNA-binding transcriptional regulator OxyR -
  ISX51_RS02635 (ISX51_02635) - 528045..528224 (+) 180 WP_281053318.1 hypothetical protein -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 23414.97 Da        Isoelectric Point: 10.0012

>NTDB_id=670469 ISX51_RS02615 WP_281053317.1 522706..523320(-) (pilN) [Vibrio lentus strain ED252]
MLHQINLLPWRDEIRAQHKKRFVHLVILGVIIALGGQWAVGNYFHAQQDKQQARLTYLKQYIAQLDRQIKSLKVAEQEHK
AILTRLDVVESLQLGRNKTTDFMNLMPELIPEGVYVDKIKMNGQEIEMSGISDSTARLATMLDNLERSESLKSVEMHSIV
HNRKRFNKEFQTFKVSFVFSPVSLDNTTAESTTAKIREKGANHG

Nucleotide


Download         Length: 615 bp        

>NTDB_id=670469 ISX51_RS02615 WP_281053317.1 522706..523320(-) (pilN) [Vibrio lentus strain ED252]
ATGTTGCATCAAATTAACCTGTTGCCTTGGCGTGATGAGATTCGCGCTCAGCACAAAAAGCGCTTTGTTCATCTGGTCAT
TCTTGGCGTCATCATCGCGCTTGGAGGGCAATGGGCGGTGGGCAACTATTTCCATGCTCAGCAAGACAAGCAGCAAGCAC
GTCTTACTTACCTAAAGCAATACATTGCCCAACTCGACCGACAAATTAAGTCGTTGAAAGTCGCGGAGCAAGAACATAAA
GCGATCCTGACTCGACTCGACGTTGTGGAATCCTTACAGCTTGGTCGCAATAAGACCACTGATTTTATGAACTTGATGCC
GGAGCTGATTCCTGAGGGCGTCTATGTCGACAAGATAAAGATGAATGGTCAGGAGATTGAAATGTCGGGCATCAGTGACA
GTACTGCTCGTCTTGCGACTATGTTGGATAACTTGGAGCGCTCTGAGTCACTTAAAAGTGTCGAGATGCATTCCATTGTT
CATAACCGTAAGCGCTTCAATAAGGAGTTCCAGACCTTCAAGGTCTCTTTTGTGTTTAGTCCTGTTTCTTTAGACAACAC
AACCGCAGAGAGCACAACCGCGAAGATAAGAGAGAAGGGGGCGAACCATGGCTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-174)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

65.217

90.196

0.588
  pilN Vibrio cholerae strain A1552

64.674

90.196

0.583