Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MTP24_RS13885 Genome accession   NZ_CP094441
Coordinates   3004530..3004946 (+) Length   138 a.a.
NCBI ID   WP_279388985.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. APM04     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2999530..3009946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTP24_RS13860 (MTP24_13860) pdhR 3000385..3001134 (-) 750 WP_243531713.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  MTP24_RS13865 (MTP24_13865) ampE 3001521..3002372 (-) 852 WP_243531715.1 beta-lactamase regulator AmpE -
  MTP24_RS13870 (MTP24_13870) ampD 3002386..3002940 (-) 555 WP_243533726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MTP24_RS13875 (MTP24_13875) nadC 3003157..3004002 (+) 846 WP_243531718.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MTP24_RS13885 (MTP24_13885) pilA 3004530..3004946 (+) 417 WP_279388985.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  MTP24_RS13890 (MTP24_13890) pilC 3005015..3006244 (+) 1230 WP_243531720.1 type II secretion system F family protein Machinery gene
  MTP24_RS13895 (MTP24_13895) pilD 3006392..3007285 (+) 894 WP_243531722.1 A24 family peptidase Machinery gene
  MTP24_RS13900 (MTP24_13900) coaE 3007303..3007935 (+) 633 WP_203985175.1 dephospho-CoA kinase -
  MTP24_RS13905 (MTP24_13905) pilB 3008068..3009744 (+) 1677 WP_243531725.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14326.59 Da        Isoelectric Point: 4.8536

>NTDB_id=669470 MTP24_RS13885 WP_279388985.1 3004530..3004946(+) (pilA) [Pseudoalteromonas sp. APM04]
MKTMTQKQQGFTLIELMIVVAIIGILAAVALPAYQNYTKKAYFSEVILATAPYKAGVQTCILTNAIADCDTGSNGIPATT
ATKVVASVSVTDGVILVTPKAVNGVLATDTYTLTPVGATNAGDQITSWTETCANGIYC

Nucleotide


Download         Length: 417 bp        

>NTDB_id=669470 MTP24_RS13885 WP_279388985.1 3004530..3004946(+) (pilA) [Pseudoalteromonas sp. APM04]
ATGAAAACAATGACTCAAAAACAACAGGGTTTCACCCTAATTGAATTAATGATTGTAGTAGCAATCATCGGTATTCTTGC
AGCGGTAGCATTGCCTGCATATCAAAATTACACCAAAAAAGCATATTTTTCAGAGGTAATTTTAGCAACTGCGCCTTATA
AAGCTGGTGTTCAAACTTGCATATTAACTAATGCAATCGCTGACTGTGATACTGGTTCTAATGGTATACCTGCAACTACA
GCAACAAAAGTTGTAGCATCTGTTTCAGTTACTGACGGCGTAATACTAGTAACACCTAAAGCAGTGAATGGTGTTTTAGC
AACAGATACATATACATTAACGCCTGTAGGGGCTACAAATGCAGGGGATCAGATAACTAGCTGGACTGAAACCTGTGCTA
ATGGTATCTACTGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-129)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

51.095

99.275

0.507
  pilA Haemophilus influenzae Rd KW20

49.63

97.826

0.486
  pilA/pilA1 Eikenella corrodens VA1

41.892

100

0.449
  pilA Vibrio parahaemolyticus RIMD 2210633

41.007

100

0.413
  pilA Glaesserella parasuis strain SC1401

43.511

94.928

0.413
  pilA2 Legionella pneumophila str. Paris

42.308

94.203

0.399
  pilA2 Legionella pneumophila strain ERS1305867

41.538

94.203

0.391

Multiple sequence alignment