Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MTP24_RS13905 Genome accession   NZ_CP094441
Coordinates   3008068..3009744 (+) Length   558 a.a.
NCBI ID   WP_243531725.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. APM04     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3003068..3014744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTP24_RS13875 (MTP24_13875) nadC 3003157..3004002 (+) 846 WP_243531718.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MTP24_RS13885 (MTP24_13885) pilA 3004530..3004946 (+) 417 WP_279388985.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  MTP24_RS13890 (MTP24_13890) pilC 3005015..3006244 (+) 1230 WP_243531720.1 type II secretion system F family protein Machinery gene
  MTP24_RS13895 (MTP24_13895) pilD 3006392..3007285 (+) 894 WP_243531722.1 A24 family peptidase Machinery gene
  MTP24_RS13900 (MTP24_13900) coaE 3007303..3007935 (+) 633 WP_203985175.1 dephospho-CoA kinase -
  MTP24_RS13905 (MTP24_13905) pilB 3008068..3009744 (+) 1677 WP_243531725.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MTP24_RS13910 (MTP24_13910) yacG 3009812..3010042 (+) 231 WP_006794561.1 DNA gyrase inhibitor YacG -
  MTP24_RS13915 (MTP24_13915) - 3010124..3010615 (-) 492 WP_203985191.1 Spy/CpxP family protein refolding chaperone -
  MTP24_RS13920 (MTP24_13920) - 3010799..3011488 (+) 690 WP_203985199.1 response regulator -
  MTP24_RS13925 (MTP24_13925) - 3011466..3012881 (+) 1416 WP_203985207.1 ATP-binding protein -
  MTP24_RS13930 (MTP24_13930) - 3012885..3013832 (+) 948 WP_243531729.1 alpha/beta hydrolase -
  MTP24_RS13935 (MTP24_13935) - 3013792..3014658 (-) 867 WP_243533731.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61939.12 Da        Isoelectric Point: 5.2452

>NTDB_id=669473 MTP24_RS13905 WP_243531725.1 3008068..3009744(+) (pilB) [Pseudoalteromonas sp. APM04]
MNINSPLLRKFLTLGRIDADTVKAKQQEFASTAELISKCGKIDSKELAEQCIDLFRVPYFDLKDFDPASIPADLVKEKLI
RKHHILPLVQKGRKVYVAASDPTDYGAFENFEFSTGLSCEIVVVDYIQLDNKIEQLLDATGSLNLSDDEFKEFADLETES
QKETAPQDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLATKLSARIKVMSRLDI
AEKRKPQDGRIKLKITERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEEEQKKLYMDALGQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGINQVQINPKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTAEQLPSEELSRQGFSTAQIEDITL
YAPKGCDGCTDGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIAEVSLKAGFNNLRLSGLRKAADGLTSLAEINRVTNM

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=669473 MTP24_RS13905 WP_243531725.1 3008068..3009744(+) (pilB) [Pseudoalteromonas sp. APM04]
ATGAATATTAACTCACCGTTATTAAGAAAGTTTCTTACGCTTGGGCGTATTGATGCCGATACGGTAAAAGCAAAGCAGCA
AGAGTTTGCCTCAACCGCTGAACTGATTTCTAAATGCGGAAAAATAGACAGTAAAGAGTTAGCAGAGCAATGTATTGATT
TGTTTCGTGTTCCCTATTTTGATTTAAAAGACTTTGATCCTGCATCTATTCCTGCCGATCTTGTAAAAGAAAAACTGATC
CGCAAACACCATATTTTACCGTTGGTACAAAAAGGCCGAAAAGTGTATGTGGCGGCCTCTGATCCAACCGATTACGGCGC
CTTCGAAAACTTTGAATTTAGTACCGGCCTTTCATGTGAAATAGTCGTCGTTGATTACATTCAGCTCGATAATAAAATTG
AGCAATTGCTAGATGCAACCGGCAGCCTAAATTTAAGCGATGATGAGTTTAAAGAATTTGCCGACCTAGAGACAGAAAGC
CAAAAAGAAACCGCCCCACAAGATGACGACAAAGACGATGCCCCAATTATTGTGTACATTAATAAAATTTTAATGGACGC
GATAAAAAAAGGCGCATCGGATCTACATTTTGAACCTTACGAGCATAAGTACCGAATTCGTTTTCGTATTGATGGTATTT
TGCACGAAGTGGCAAGCCCGCCTAATACATTAGCAACCAAGCTTTCTGCTCGTATTAAAGTAATGTCGCGACTTGATATT
GCCGAAAAGCGTAAACCACAAGATGGCCGAATTAAATTAAAAATTACTGAACGAAAAAGTATCGATTTTCGTGTCAGTAC
TATGCCCACGCTGTGGGGCGAAAAAATAGTAATGCGTATTCTTGATTCATCAAGCGCTATGCTAGGCATTGATGTACTTG
GTTACGAAGAAGAGCAGAAAAAGTTATACATGGATGCACTTGGTCAACCACAAGGCATGATACTTGTTACAGGTCCTACC
GGGTCAGGTAAAACGGTATCGCTTTATACGGGTTTAAACATATTAAACCAACCTGAGCGAAACATTAGTACCGCTGAGGA
TCCAGTTGAGATAAATCTTGAAGGGATAAACCAAGTACAAATTAACCCTAAAGCCGACATGACCTTTGCTAATGCACTGC
GTGCGTTTTTACGTCAAGATCCCGATGTAGTTATGGTAGGTGAAATACGTGACCTTGAAACCGCAGAAATTTCAATAAAA
GCGGCGCAAACAGGTCACTTGGTTTTATCTACATTACATACCAATTCAGCGCCAGAAACCATTACCCGACTATTAAATAT
GGGCGTACCCGCCTATAACGTAGCCAGCTCAATTAGTTTAATTATTGCACAGCGACTAGCACGTCGTTTATGCCCTAAAT
GTAAAACGGCAGAACAATTGCCAAGCGAAGAGCTCTCTCGTCAAGGATTTAGTACAGCGCAAATTGAAGATATAACTTTA
TATGCACCTAAAGGGTGTGATGGCTGTACCGATGGTTACAAAGGCCGTGTAGGTATTTACGAAGTTATGCAAATCACACC
AGAAATTGCACAAATAATCATGCGCGGTGGTAACTCACTAGAAATCGCTGAGGTATCATTAAAAGCCGGTTTTAATAATT
TGCGCCTATCGGGTTTACGTAAAGCCGCTGATGGTTTAACGTCACTTGCTGAAATAAACCGCGTAACTAATATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.452

100

0.565
  pilB Acinetobacter baylyi ADP1

58.416

90.502

0.529
  pilB Legionella pneumophila strain ERS1305867

53.05

96.953

0.514
  pilB Vibrio cholerae strain A1552

53.296

95.161

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

52.919

95.161

0.504
  pilB Vibrio campbellii strain DS40M4

52.542

95.161

0.5
  pilF Neisseria gonorrhoeae MS11

50.192

93.548

0.47
  pilB/pilB1 Synechocystis sp. PCC 6803

37.46

100

0.418
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.13

94.803

0.371

Multiple sequence alignment