Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSCI_RS05010 Genome accession   NZ_AP014637
Coordinates   1083033..1084253 (+) Length   406 a.a.
NCBI ID   WP_045483602.1    Uniprot ID   A0A077LBE1
Organism   Pseudomonas sp. StFLB209     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1078033..1089253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSCI_RS04990 (PSCI_0989) - 1078416..1079816 (-) 1401 WP_045483576.1 amino acid permease -
  PSCI_RS04995 (PSCI_0990) - 1080148..1080495 (-) 348 WP_045483578.1 cupin domain-containing protein -
  PSCI_RS05000 (PSCI_0991) - 1080669..1081109 (-) 441 WP_045483582.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PSCI_RS05005 (PSCI_0992) pilB 1081333..1083030 (+) 1698 WP_045483599.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSCI_RS05010 (PSCI_0993) pilC 1083033..1084253 (+) 1221 WP_045483602.1 type II secretion system F family protein Machinery gene
  PSCI_RS05015 (PSCI_0994) pilD 1084255..1085127 (+) 873 WP_045483605.1 A24 family peptidase Machinery gene
  PSCI_RS05020 (PSCI_0995) coaE 1085124..1085744 (+) 621 WP_045483609.1 dephospho-CoA kinase -
  PSCI_RS05025 (PSCI_0996) yacG 1085744..1085953 (+) 210 WP_045483612.1 DNA gyrase inhibitor YacG -
  PSCI_RS05030 (PSCI_0997) - 1086072..1086539 (-) 468 WP_045483615.1 hypothetical protein -
  PSCI_RS05035 (PSCI_0998) - 1086640..1087269 (+) 630 WP_045483618.1 DUF1780 domain-containing protein -
  PSCI_RS05040 (PSCI_0999) - 1087269..1087766 (+) 498 WP_045493854.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44327.21 Da        Isoelectric Point: 10.0099

>NTDB_id=66430 PSCI_RS05010 WP_045483602.1 1083033..1084253(+) (pilC) [Pseudomonas sp. StFLB209]
MAKKAVKLSTFNWEGLDRKGAKVRGEMDGHNPALIKAQLRKQGINPTKVRKKSTSIFGSRGKKIKPLDIAFFSRQMATMM
KAGVPLLQSFDIIAEGSEKPAMRVLVESLKQDVAAGNSFATALRQKPQYFDELYCNLVDAGEQAGALEALLDRVATYKEK
TEALKAKIKKAMTYPAAVMIVAAIVSSILLIKVVPQFKSVFEGFGAELPAFTLMVIGLSEVFEQWWYIILGGLIGGIFLF
LRSYNNSQKFRDTVDRFLLKLPLVGPLIYKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFKNAVYKVKQDVATG
MQLNFSMRSTNVFPSLAIQMTAIGEESGALDSMLDKVATYYEDEVDNMVNNLTTLMEPMIMAVLGVIVGGLVIAMYLPIF
KLGAAV

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=66430 PSCI_RS05010 WP_045483602.1 1083033..1084253(+) (pilC) [Pseudomonas sp. StFLB209]
ATGGCCAAGAAGGCAGTCAAACTCAGTACCTTCAATTGGGAAGGCCTGGACCGCAAAGGCGCCAAGGTGCGTGGCGAGAT
GGACGGTCACAACCCGGCACTGATCAAGGCCCAGTTGCGCAAGCAGGGCATCAACCCGACCAAGGTGCGCAAAAAATCCA
CCTCGATCTTTGGCAGCCGGGGCAAAAAGATCAAACCGCTGGATATCGCCTTCTTCTCACGGCAGATGGCGACCATGATG
AAAGCCGGCGTGCCGCTGCTGCAGTCGTTCGACATCATTGCCGAAGGCTCGGAAAAACCGGCCATGCGCGTGCTGGTCGA
GTCGCTGAAGCAGGACGTGGCAGCCGGTAACAGCTTTGCCACCGCACTGCGCCAGAAGCCGCAGTATTTCGACGAGCTGT
ATTGCAACCTGGTGGACGCCGGCGAACAGGCCGGTGCCCTTGAAGCGTTGCTGGACCGGGTCGCCACCTACAAGGAAAAA
ACCGAAGCGCTCAAGGCCAAGATCAAGAAAGCCATGACCTATCCGGCAGCGGTGATGATAGTGGCGGCCATCGTCTCGTC
GATTCTGTTGATCAAGGTCGTGCCGCAATTCAAGTCGGTGTTCGAAGGCTTCGGTGCCGAGCTACCGGCCTTTACCCTGA
TGGTGATTGGCCTTTCCGAAGTATTTGAACAATGGTGGTACATCATCCTCGGCGGGCTGATCGGCGGGATTTTTCTGTTC
CTGCGCAGCTACAACAACTCGCAGAAATTCCGCGATACGGTTGACCGTTTTCTGCTCAAGCTACCGCTGGTAGGCCCGCT
GATCTACAAGTCCTCCGTGGCGCGCTATGCCCGCACCCTGGCCACGACCTTTGCCGCCGGCGTGCCGCTGGTCGAAGCGC
TGGACTCGGTGGCCGGTGCGACCGGCAACGTGGTGTTCAAGAATGCGGTATACAAGGTCAAGCAGGACGTGGCGACCGGT
ATGCAACTGAACTTCTCGATGCGCTCGACCAATGTATTTCCCAGCCTGGCGATCCAGATGACCGCCATCGGCGAAGAATC
TGGCGCACTGGACAGCATGCTCGACAAGGTCGCGACCTACTACGAAGACGAAGTGGACAATATGGTCAATAACCTCACCA
CCCTGATGGAGCCGATGATCATGGCGGTACTGGGGGTGATTGTCGGCGGCCTGGTGATCGCCATGTACCTGCCGATCTTC
AAACTGGGGGCGGCGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(277-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A077LBE1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.645

100

0.736
  pilC Acinetobacter baylyi ADP1

59.413

100

0.599
  pilC Acinetobacter baumannii D1279779

59.506

99.754

0.594
  pilC Legionella pneumophila strain ERS1305867

53.695

100

0.537
  pilG Neisseria gonorrhoeae MS11

42.928

99.261

0.426
  pilG Neisseria meningitidis 44/76-A

42.928

99.261

0.426
  pilC Vibrio campbellii strain DS40M4

41.278

100

0.414
  pilC Vibrio cholerae strain A1552

41.337

99.507

0.411
  pilC Thermus thermophilus HB27

37.469

99.261

0.372

Multiple sequence alignment