Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSCI_RS05005 Genome accession   NZ_AP014637
Coordinates   1081333..1083030 (+) Length   565 a.a.
NCBI ID   WP_045483599.1    Uniprot ID   -
Organism   Pseudomonas sp. StFLB209     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1076333..1088030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSCI_RS04980 (PSCI_0987) - 1076946..1077785 (+) 840 WP_045483572.1 AraC family transcriptional regulator -
  PSCI_RS04985 (PSCI_0988) - 1077826..1078419 (+) 594 WP_045483574.1 LysE family translocator -
  PSCI_RS04990 (PSCI_0989) - 1078416..1079816 (-) 1401 WP_045483576.1 amino acid permease -
  PSCI_RS04995 (PSCI_0990) - 1080148..1080495 (-) 348 WP_045483578.1 cupin domain-containing protein -
  PSCI_RS05000 (PSCI_0991) - 1080669..1081109 (-) 441 WP_045483582.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PSCI_RS05005 (PSCI_0992) pilB 1081333..1083030 (+) 1698 WP_045483599.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSCI_RS05010 (PSCI_0993) pilC 1083033..1084253 (+) 1221 WP_045483602.1 type II secretion system F family protein Machinery gene
  PSCI_RS05015 (PSCI_0994) pilD 1084255..1085127 (+) 873 WP_045483605.1 A24 family peptidase Machinery gene
  PSCI_RS05020 (PSCI_0995) coaE 1085124..1085744 (+) 621 WP_045483609.1 dephospho-CoA kinase -
  PSCI_RS05025 (PSCI_0996) yacG 1085744..1085953 (+) 210 WP_045483612.1 DNA gyrase inhibitor YacG -
  PSCI_RS05030 (PSCI_0997) - 1086072..1086539 (-) 468 WP_045483615.1 hypothetical protein -
  PSCI_RS05035 (PSCI_0998) - 1086640..1087269 (+) 630 WP_045483618.1 DUF1780 domain-containing protein -
  PSCI_RS05040 (PSCI_0999) - 1087269..1087766 (+) 498 WP_045493854.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 62203.38 Da        Isoelectric Point: 6.4523

>NTDB_id=66429 PSCI_RS05005 WP_045483599.1 1081333..1083030(+) (pilB) [Pseudomonas sp. StFLB209]
MSDVVLSGLAKQLVASELLTEKAAQQASQQARRDKVSLITYLVQHKLVKSLTLAEMASEQFGVPFMDLHSLDKDGQPKGL
VSEKLVRQHHALPLWRRGNKLFIGVSDPTNHQAVTDIQFSTGLNTEAILVEDDKLSDAIERLFDTGGGLAEIGDIDLNVD
LEPTDNLKETSLSAQSDADDAPVVRFVNKMLLDAIRLGSSDLHFEPYEKSYRVRFRTDGILHEAAKPPIQLAGRIAARLK
VMASLDISERRKPQDGRVKLRISNNKAIDFRMNTLPTLWGEKIVMRILDSASAKMGIDALGYEPDQKALYLEALKQPQGM
ILVTGPTGSGKTVSLYTGLNILNTVGINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRDLE
TAEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVASFNIATAINLIIAQRLARKLCKHCRKEIEVPHDTLLKEGFSEA
QIGTFTLYAPVGCEQCNGGYKGRVGIYEVVKKTPELERIIMEEGNALQIAAQMRKDGFNDLRTSGLVKVMQGVTSLEEVN
RVTKD

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=66429 PSCI_RS05005 WP_045483599.1 1081333..1083030(+) (pilB) [Pseudomonas sp. StFLB209]
ATGTCTGATGTTGTCCTCAGCGGTCTGGCCAAGCAACTGGTCGCTTCCGAGCTGTTAACCGAAAAAGCCGCCCAGCAGGC
CTCCCAGCAGGCGCGCCGCGACAAGGTTTCGCTGATTACCTATCTGGTCCAGCACAAGCTGGTGAAAAGCCTGACACTGG
CCGAGATGGCTTCCGAGCAATTCGGTGTGCCATTCATGGACCTGCACAGCCTCGACAAGGACGGCCAGCCCAAAGGACTG
GTCAGCGAAAAGCTGGTGCGCCAGCATCACGCCCTGCCGCTCTGGCGGCGCGGCAACAAGCTGTTCATTGGCGTGTCGGA
CCCGACCAACCATCAAGCTGTTACCGATATCCAGTTCAGCACCGGCCTGAATACCGAAGCCATTCTGGTTGAAGACGATA
AGCTCAGCGATGCCATCGAGCGCCTGTTCGATACCGGTGGGGGCCTTGCAGAAATTGGCGACATTGATCTAAACGTCGAT
CTGGAGCCTACCGATAATCTCAAAGAAACATCGCTCAGCGCCCAAAGCGATGCCGACGACGCGCCGGTGGTGCGCTTCGT
CAACAAGATGCTGCTCGATGCCATTCGCCTGGGCTCTTCGGACCTGCACTTCGAGCCCTATGAAAAGAGCTACCGGGTAC
GCTTTCGCACCGATGGCATCCTGCATGAAGCCGCCAAACCGCCGATTCAGTTGGCCGGGCGGATTGCCGCACGCCTGAAG
GTGATGGCCAGCCTGGATATTTCCGAGCGGCGCAAGCCGCAGGACGGGCGGGTCAAGTTGCGCATTTCCAATAACAAGGC
CATCGATTTCCGGATGAACACCCTGCCCACTCTGTGGGGCGAAAAGATCGTGATGCGGATTCTCGACTCTGCCAGTGCGA
AGATGGGTATCGACGCCCTGGGCTACGAACCGGATCAGAAAGCCCTGTACCTGGAAGCGCTCAAGCAACCGCAAGGCATG
ATTCTGGTCACCGGCCCGACCGGCTCGGGCAAGACCGTGTCGCTGTACACCGGCCTGAATATTCTCAATACCGTGGGCAT
CAATATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTCGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGGGCA
TGGACTTCGCCCAGGCCCTGCGAGCGTTTTTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTGGAA
ACCGCCGAAATCGCTATCAAGGCCTCGCAGACCGGCCATATGGTGCTCTCGACCCTGCACACCAACAGCGCGGCAGAAAC
CCTTACGCGCCTGCACCATATGGGCGTGGCTTCGTTCAACATTGCCACCGCGATCAACCTGATCATTGCCCAGCGGCTGG
CCCGCAAGCTGTGCAAGCATTGCCGCAAAGAGATCGAAGTGCCGCACGACACCCTGCTTAAAGAAGGCTTCAGCGAGGCG
CAGATCGGCACCTTTACCCTGTACGCGCCGGTCGGTTGCGAGCAGTGCAATGGCGGCTATAAGGGCCGGGTGGGGATCTA
CGAGGTGGTGAAGAAAACCCCGGAACTGGAACGGATCATCATGGAAGAAGGCAATGCCCTGCAAATCGCCGCGCAGATGC
GCAAGGACGGCTTCAATGACCTGCGCACCTCTGGTCTGGTGAAAGTGATGCAGGGCGTTACCAGCCTGGAAGAAGTCAAC
CGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.23

100

0.582
  pilB Acinetobacter baylyi ADP1

57.904

99.646

0.577
  pilB Legionella pneumophila strain ERS1305867

54.577

100

0.549
  pilB Vibrio cholerae strain A1552

51.515

99.292

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

50.442

100

0.504
  pilB Vibrio campbellii strain DS40M4

49.376

99.292

0.49
  pilF Neisseria gonorrhoeae MS11

48.577

99.469

0.483
  pilF Thermus thermophilus HB27

38.624

100

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.076

93.628

0.375

Multiple sequence alignment