Detailed information NTG0066431

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PSCI_RS05015
• Genome accession: NZ_AP014637
• Coordinates: 1084255..1085127 (+)
• Length: 290 a.a.
• NCBI ID: WP_045483605.1
• Uniprot ID: A0A077LD00
• Organism: Pseudomonas sp. StFLB209
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1079255..1090127

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSCI_RS04995 (PSCI_0990)	-	1080148..1080495 (-)	348	WP_045483578.1	cupin domain-containing protein	-
PSCI_RS05000 (PSCI_0991)	-	1080669..1081109 (-)	441	WP_045483582.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
PSCI_RS05005 (PSCI_0992)	pilB	1081333..1083030 (+)	1698	WP_045483599.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PSCI_RS05010 (PSCI_0993)	pilC	1083033..1084253 (+)	1221	WP_045483602.1	type II secretion system F family protein	Machinery gene
PSCI_RS05015 (PSCI_0994)	pilD	1084255..1085127 (+)	873	WP_045483605.1	A24 family peptidase	Machinery gene
PSCI_RS05020 (PSCI_0995)	coaE	1085124..1085744 (+)	621	WP_045483609.1	dephospho-CoA kinase	-
PSCI_RS05025 (PSCI_0996)	yacG	1085744..1085953 (+)	210	WP_045483612.1	DNA gyrase inhibitor YacG	-
PSCI_RS05030 (PSCI_0997)	-	1086072..1086539 (-)	468	WP_045483615.1	hypothetical protein	-
PSCI_RS05035 (PSCI_0998)	-	1086640..1087269 (+)	630	WP_045483618.1	DUF1780 domain-containing protein	-
PSCI_RS05040 (PSCI_0999)	-	1087269..1087766 (+)	498	WP_045493854.1	MOSC domain-containing protein	-
PSCI_RS05045 (PSCI_1000)	-	1088019..1089317 (+)	1299	WP_045483621.1	NAD(P)/FAD-dependent oxidoreductase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31861.06 Da
• Isoelectric Point: 8.3904

>NTDB_id=66431 PSCI_RS05015 WP_045483605.1 1084255..1085127(+) (pilD) [Pseudomonas sp. StFLB209]
MSLAEFLAASPAAFIVCTLLLGLLVGSFLNVVIHRLPKMMLRDWQTQAREVLELPAEPAPARYNLILPHSACPHCEHRVR
PWENVPVISWLLLKGRCSSCRAPISKRYPLVELACGVLSAGVAWHFGFGWQCAAMLVLGWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNSFGLFTTLGDALWGAVAGYLSLWTVFWLFKLLTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
ALLGMILLRLRRHQAGTPMPFGPYLAIAGWIALLWGGQITDSYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=66431 PSCI_RS05015 WP_045483605.1 1084255..1085127(+) (pilD) [Pseudomonas sp. StFLB209]
ATGTCACTGGCCGAATTTCTGGCCGCCTCCCCGGCGGCCTTTATCGTTTGCACCCTGCTGCTCGGCCTGCTGGTCGGCAG
CTTCCTGAATGTGGTGATCCACCGCCTGCCGAAGATGATGCTGCGCGACTGGCAGACTCAGGCCCGTGAAGTGCTTGAGC
TGCCCGCAGAACCGGCCCCGGCGCGCTACAACCTGATCCTGCCGCATTCCGCTTGCCCGCATTGTGAACACCGTGTCCGC
CCCTGGGAAAACGTGCCGGTCATCAGTTGGTTGCTGCTCAAGGGCCGCTGTTCCAGTTGCCGGGCGCCGATCAGTAAACG
CTACCCGTTGGTCGAGCTGGCGTGCGGGGTATTATCGGCCGGCGTCGCCTGGCACTTCGGCTTCGGCTGGCAATGCGCAG
CAATGCTGGTGCTGGGCTGGGGTCTGTTGGCCATGAGCCTGATCGATGCCGATCACCAGTTGCTCCCCGACAGCCTGGTG
CTGCCGCTGTTGTGGCTGGGCCTGATCGTCAACAGCTTCGGCCTGTTCACGACCCTCGGCGATGCGCTGTGGGGGGCGGT
GGCCGGTTACCTGTCGTTGTGGACGGTGTTCTGGCTATTCAAGCTGCTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGCTATTGGCCATGCTCGGCGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATTTTGCTGTCGTCACTGGTCGGC
GCGCTGCTGGGCATGATTTTGCTGCGTCTGCGCCGCCATCAGGCGGGCACGCCAATGCCTTTTGGTCCTTATCTGGCCAT
CGCGGGGTGGATCGCTTTGCTATGGGGTGGTCAAATAACCGACTCCTATCTGCAATTTGCCGGTTTTAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

3D structure

• AlphaFold DB: A0A077LD00

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.191	97.241	0.517
pilD	Vibrio campbellii strain DS40M4	51.418	97.241	0.5
pilD	Acinetobacter baumannii D1279779	49.825	98.276	0.49
pilD	Acinetobacter nosocomialis M2	49.123	98.276	0.483
pilD	Neisseria gonorrhoeae MS11	52.174	87.241	0.455