Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MME58_RS03755 Genome accession   NZ_CP092923
Coordinates   843663..844880 (-) Length   405 a.a.
NCBI ID   WP_055007276.1    Uniprot ID   A0A0Q0E2I9
Organism   Pseudomonas syringae pv. tagetis strain ICMP 4091     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 838663..849880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MME58_RS03725 (MME58_03725) - 838892..839359 (+) 468 WP_055005980.1 hypothetical protein -
  MME58_RS03730 (MME58_03730) - 839750..841270 (+) 1521 WP_055005981.1 tetratricopeptide repeat protein -
  MME58_RS03735 (MME58_03735) - 841375..841851 (+) 477 WP_241409118.1 hypothetical protein -
  MME58_RS03740 (MME58_03740) yacG 841963..842172 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  MME58_RS03745 (MME58_03745) coaE 842169..842792 (-) 624 WP_055007274.1 dephospho-CoA kinase -
  MME58_RS03750 (MME58_03750) pilD 842789..843661 (-) 873 WP_055007275.1 prepilin peptidase Machinery gene
  MME58_RS03755 (MME58_03755) pilC 843663..844880 (-) 1218 WP_055007276.1 type II secretion system F family protein Machinery gene
  MME58_RS03760 (MME58_03760) pilB 844883..846577 (-) 1695 WP_055007277.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MME58_RS03765 (MME58_03765) - 846802..847230 (+) 429 WP_055007278.1 pilin -
  MME58_RS27540 - 847458..847827 (-) 370 Protein_744 winged helix-turn-helix transcriptional regulator -
  MME58_RS27545 - 847980..848586 (+) 607 Protein_745 glutathione S-transferase family protein -
  MME58_RS03785 (MME58_03785) - 848826..849518 (+) 693 WP_055007280.1 DUF433 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44002.94 Da        Isoelectric Point: 10.1387

>NTDB_id=661152 MME58_RS03755 WP_055007276.1 843663..844880(-) (pilC) [Pseudomonas syringae pv. tagetis strain ICMP 4091]
MASKAVKVIVYTWEGVDKKGVKLSGELSGHNPALIKAQLRKQGVNPTKVRKKAASIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIIGEGAENPNMRALVGSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPAAVLIVALIVSGILLIKVVPQFQSVFAGFGAELPTFTLMVISLSEIVQKWWLAIAGMFFFSIFIFK
RAYNKSQKFRDSLDRLLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVNKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDNMLDKVATYYEDEVDNMVDSLTSLMEPMIMAVLGVVVGGLVIAMYLPIFK
LGDVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=661152 MME58_RS03755 WP_055007276.1 843663..844880(-) (pilC) [Pseudomonas syringae pv. tagetis strain ICMP 4091]
ATGGCCAGCAAGGCAGTCAAAGTCATCGTTTACACATGGGAAGGCGTGGACAAAAAAGGCGTCAAACTTAGCGGAGAATT
AAGCGGCCACAATCCGGCGCTTATCAAAGCACAATTGCGTAAACAGGGTGTCAACCCTACCAAGGTGCGCAAAAAAGCTG
CCTCGATCTTCGGCAAGGGTAAAAAAATCAAGCCTCTAGACATCGCTTTTTTCTCTCGGCAAATGGCGACGATGATGAAA
GCCGGAGTTCCGCTGCTACAGTCGTTCGATATTATCGGTGAGGGTGCAGAAAATCCGAATATGCGAGCGCTGGTAGGCTC
ACTCAAACAAGAAGTATCAGCAGGTAACAGTTTTGCAACGGCTTTGCGGCAAAAGCCCGAGTACTTTGATGATCTCTTTT
GCAACCTCGTGGATGCGGGCGAGCAAGCTGGTGCTCTTGAGAGTCTGCTGGATCGAGTAGCAAGCTACAAAGAAAAGACA
GAAAAGCTCAAAGCAAAAATAAAGAAAGCCATGACCTACCCCGCTGCTGTTTTAATCGTTGCACTTATTGTGTCCGGCAT
TCTATTAATTAAAGTAGTTCCGCAGTTTCAGTCAGTTTTTGCAGGCTTTGGTGCAGAACTCCCTACGTTCACATTGATGG
TTATAAGTCTTTCAGAGATTGTGCAGAAATGGTGGCTGGCGATTGCAGGCATGTTTTTCTTCAGCATTTTCATATTCAAA
CGTGCTTATAATAAGTCTCAGAAATTTCGCGACAGCCTTGACCGTCTTTTGCTGAAAGTCCCCATCATTGGGCCGCTGAT
TTTTAAGTCATCAGTAGCGCGTTATGCCCGCACGCTCGCAACAACTTTTGCCGCTGGAGTTCCGCTAGTAGAGGCTCTCG
ACTCTGTCGCAGGGGCAACGGGCAACGTGGTGTTCAGAAACGCTGTGAACAAGGTTAAGCAAGACGTGTCCACCGGAATG
CAGCTTAATTTCTCAATGCGCTCTACTGGAGTTTTTCCCAGTTTAGCAATTCAGATGACTGCCATTGGAGAAGAGTCTGG
TGCGTTGGATAATATGTTGGATAAGGTCGCAACTTATTATGAGGATGAGGTCGACAATATGGTAGACAGTCTGACCAGCC
TTATGGAACCTATGATTATGGCCGTTCTCGGTGTGGTAGTGGGCGGATTGGTCATAGCAATGTACTTACCGATTTTCAAG
CTCGGAGACGTTGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Q0E2I9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.321

100

0.743
  pilC Acinetobacter baylyi ADP1

59.559

100

0.6
  pilC Acinetobacter baumannii D1279779

59.901

99.753

0.598
  pilC Legionella pneumophila strain ERS1305867

51.88

98.519

0.511
  pilG Neisseria gonorrhoeae MS11

42.821

98.025

0.42
  pilG Neisseria meningitidis 44/76-A

42.569

98.025

0.417
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilC Vibrio campbellii strain DS40M4

42.317

98.025

0.415
  pilC Thermus thermophilus HB27

37.406

99.012

0.37