Detailed information NTG0661151

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MME58_RS03750
• Genome accession: NZ_CP092923
• Coordinates: 842789..843661 (-)
• Length: 290 a.a.
• NCBI ID: WP_055007275.1
• Uniprot ID: -
• Organism: Pseudomonas syringae pv. tagetis strain ICMP 4091
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 837789..848661

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MME58_RS03720 (MME58_03720)	-	838135..838764 (-)	630	WP_003345130.1	DUF1780 domain-containing protein	-
MME58_RS03725 (MME58_03725)	-	838892..839359 (+)	468	WP_055005980.1	hypothetical protein	-
MME58_RS03730 (MME58_03730)	-	839750..841270 (+)	1521	WP_055005981.1	tetratricopeptide repeat protein	-
MME58_RS03735 (MME58_03735)	-	841375..841851 (+)	477	WP_241409118.1	hypothetical protein	-
MME58_RS03740 (MME58_03740)	yacG	841963..842172 (-)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
MME58_RS03745 (MME58_03745)	coaE	842169..842792 (-)	624	WP_055007274.1	dephospho-CoA kinase	-
MME58_RS03750 (MME58_03750)	pilD	842789..843661 (-)	873	WP_055007275.1	prepilin peptidase	Machinery gene
MME58_RS03755 (MME58_03755)	pilC	843663..844880 (-)	1218	WP_055007276.1	type II secretion system F family protein	Machinery gene
MME58_RS03760 (MME58_03760)	pilB	844883..846577 (-)	1695	WP_055007277.1	type IV-A pilus assembly ATPase PilB	Machinery gene
MME58_RS03765 (MME58_03765)	-	846802..847230 (+)	429	WP_055007278.1	pilin	-
MME58_RS27540	-	847458..847827 (-)	370	Protein_744	winged helix-turn-helix transcriptional regulator	-
MME58_RS27545	-	847980..848586 (+)	607	Protein_745	glutathione S-transferase family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 32041.20 Da
• Isoelectric Point: 8.3694

>NTDB_id=661151 MME58_RS03750 WP_055007275.1 842789..843661(-) (pilD) [Pseudomonas syringae pv. tagetis strain ICMP 4091]
MPLLDLLASSPLAFVTTCCILGLIIGSFLNVVVYRLPLMMERDWKAQSREMLGLPAEPDQPAFNLNRPRSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGYGWQAPAMLVLSWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=661151 MME58_RS03750 WP_055007275.1 842789..843661(-) (pilD) [Pseudomonas syringae pv. tagetis strain ICMP 4091]
ATGCCTCTCCTCGACCTACTGGCCAGCTCCCCCCTGGCCTTCGTCACCACCTGCTGCATCCTCGGCCTCATCATCGGAAG
CTTCCTCAACGTGGTCGTCTACCGCCTGCCACTAATGATGGAGCGCGACTGGAAAGCCCAATCCCGCGAAATGCTCGGCC
TGCCCGCCGAACCCGATCAGCCTGCCTTCAACCTGAACCGCCCCCGCTCCAGTTGCCCGCACTGCGCGCACAAAATCCGC
CCGTGGGAAAATCTGCCGGTCATCAGCTATCTGCTGCTGCGCGGTAAATGCTCGCAGTGCAAAGCGCCGATCAGCAAACG
CTACCCGCTGGTCGAGCTGACCTGCGCAGTGTTATCAGCCTACGTCGCCTGGCATTTCGGCTACGGCTGGCAGGCGCCGG
CGATGCTGGTGTTGAGCTGGGGGCTGCTGGCGATGAGTCTGATCGACGCTGATCACCAGCTGTTACCTGATTCTCTGGTG
CTGCCATTGCTGTGGCTGGGCCTGATCGTCAACGCTTTCGGGCTGTTCACCTCGCTGAATGATGCGCTATGGGGAGCGGT
GGCCGGCTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTTGGCGCGTGGGGCGGCTGGCAGATTCTACCGTTGACCATCCTGCTGTCGTCGCTGGTGGGC
GCGGTGCTGGGGGTGATCATGATGCGTGTGCGCAGGGTCGAAAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
TGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.004	97.586	0.517
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Acinetobacter baumannii D1279779	52.92	94.483	0.5
pilD	Acinetobacter nosocomialis M2	52.19	94.483	0.493
pilD	Neisseria gonorrhoeae MS11	51.493	92.414	0.476