Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MME58_RS03760 Genome accession   NZ_CP092923
Coordinates   844883..846577 (-) Length   564 a.a.
NCBI ID   WP_055007277.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. tagetis strain ICMP 4091     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 839883..851577
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MME58_RS03735 (MME58_03735) - 841375..841851 (+) 477 WP_241409118.1 hypothetical protein -
  MME58_RS03740 (MME58_03740) yacG 841963..842172 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  MME58_RS03745 (MME58_03745) coaE 842169..842792 (-) 624 WP_055007274.1 dephospho-CoA kinase -
  MME58_RS03750 (MME58_03750) pilD 842789..843661 (-) 873 WP_055007275.1 prepilin peptidase Machinery gene
  MME58_RS03755 (MME58_03755) pilC 843663..844880 (-) 1218 WP_055007276.1 type II secretion system F family protein Machinery gene
  MME58_RS03760 (MME58_03760) pilB 844883..846577 (-) 1695 WP_055007277.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MME58_RS03765 (MME58_03765) - 846802..847230 (+) 429 WP_055007278.1 pilin -
  MME58_RS27540 - 847458..847827 (-) 370 Protein_744 winged helix-turn-helix transcriptional regulator -
  MME58_RS27545 - 847980..848586 (+) 607 Protein_745 glutathione S-transferase family protein -
  MME58_RS03785 (MME58_03785) - 848826..849518 (+) 693 WP_055007280.1 DUF433 domain-containing protein -
  MME58_RS03790 (MME58_03790) - 849515..849922 (+) 408 WP_055007281.1 hypothetical protein -
  MME58_RS03795 (MME58_03795) - 849968..850513 (-) 546 WP_122239075.1 DUF1523 family protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62530.74 Da        Isoelectric Point: 6.1206

>NTDB_id=661153 MME58_RS03760 WP_055007277.1 844883..846577(-) (pilB) [Pseudomonas syringae pv. tagetis strain ICMP 4091]
MTDAVLTGLAKQLVLAELLTEPVAQQAYQQARRDKISLVSYLVQNKLVKSLTLAELASDQFGVPFMDLASLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTTAIDRFFDSDSGLGNLEDVDLGVD
IEPADGKETSLATQNDADDAPVVRFVNKMLLDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEVARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRMSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELDIPRETLIQEGFPEEK
IGNFKIYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=661153 MME58_RS03760 WP_055007277.1 844883..846577(-) (pilB) [Pseudomonas syringae pv. tagetis strain ICMP 4091]
ATGACTGATGCTGTCCTCACTGGTTTGGCCAAGCAATTGGTCCTCGCCGAGCTCCTTACTGAACCTGTTGCGCAGCAAGC
GTATCAACAGGCGCGCCGCGACAAGATTTCGTTGGTGAGCTATCTGGTTCAGAACAAGCTGGTCAAAAGCCTCACGCTGG
CGGAATTGGCTTCTGATCAGTTCGGCGTGCCGTTCATGGACCTCGCCAGCCTGGACAAGGAAAGCCAGCCTAAAGGTCTA
GTGAGTGAAAAGCTGGTTCGGCAGCATCACGCCCTGCCGCTCTGGCGACGAGGTAACAAGCTGTTCATTGGCATATCGGA
TCCGACCAATCATCAGGCTGTGACCGATATTCAGTTCAGTACTGGCCTAAACACCGAAGCGATTCTGGTCGAAGATGACA
AGCTGACCACTGCGATAGATCGATTTTTTGACAGTGATAGCGGTCTGGGCAATCTGGAAGATGTCGATCTGGGTGTGGAT
ATCGAACCGGCTGATGGTAAGGAAACGTCCCTCGCGACGCAGAATGACGCTGACGATGCCCCGGTGGTCCGTTTCGTCAA
CAAAATGCTGTTGGATGCCATCCGGTTAGGATCGTCCGATCTACATTTCGAGCCCTACGAAAAGATCTTTCGCGTGCGCC
TGCGTACCGATGGCATTTTGCATGAGGTTGCCAGGCCGCCGATTCATCTGGCAAATCGTATTGCTGCACGCCTGAAAGTC
ATGGCGAGCCTCGACATTTCGGAACGGCGCAAACCACAGGACGGCCGGGTCAAGTTACGAATGTCCAAAACCAAAGCTAT
TGATTTTCGTATGAATACCTTGCCCACTCTATGGGGCGAGAAAATCGTGATGCGGATTCTCGACCCGACCAGCGCACAGA
TGGGCATCGATGCTCTGGGCTACGAGCCAGAGCAAAAAGCACTGTATCTGGAGGCGCTGAAACAGCCGCAGGGCATGATT
CTAGTGACCGGCCCCACGGGTTCCGGCAAGACCGTTTCTCTGTATACCGGTCTGAATATCCTCAATACTGTGGACATCAA
TATATCCACGGCCGAAGACCCGGTAGAGATCAACCTAGAAGGTATCAATCAGGTCAACGTCAACCCGCGTCAGGGGCTAG
ACTTTTCTCAAGCATTGCGCGCGTTCCTGCGTCAAGATCCCGACGTGATAATGGTCGGTGAGATACGAGACTTGGAAACG
GCAGAAATTGCCATCAAAGCCTCGCAGACCGGGCATATGGTGCTTTCCACACTGCACACCAACAGCGCAGCGGAAACGCT
GACTCGACTGCATCATATGGGTGTCGCGGCATTCAACATTGCTACGGCAATCAACCTGATTATTGCGCAACGGCTGGCAC
GTAAGCTGTGCAGCCACTGCAAGAAAGAATTGGATATTCCTCGCGAGACACTTATTCAGGAAGGTTTTCCAGAAGAGAAA
ATCGGCAATTTCAAGATATACGGCCCGGTGGGCTGTGAGCACTGCAACGGGGGTTACCGAGGCAGGGTGGGCATTTACGA
AGTGGTCAAGAAAACACCGGAACTGGAACGCATCATCATGGAAGAAGGCAACTCGCTGGAGATCTCTAGACAGATGCGCA
AGGACGGTTTCAATGACCTTCGCACATCAGGGCTTTCGAAAGCCATGCAGGGCATCACCAGCCTTGAAGAAGTCAACCGC
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.025

100

0.583
  pilB Acinetobacter baylyi ADP1

57.473

99.645

0.573
  pilB Legionella pneumophila strain ERS1305867

55.575

100

0.557
  pilB Vibrio cholerae strain A1552

51.068

99.645

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

50.719

98.582

0.5
  pilB Vibrio campbellii strain DS40M4

49.47

100

0.496
  pilF Neisseria gonorrhoeae MS11

48.399

99.645

0.482
  pilF Thermus thermophilus HB27

38.693

100

0.388