Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MKR81_RS01610 Genome accession   NZ_CP092682
Coordinates   338775..340523 (+) Length   582 a.a.
NCBI ID   WP_240305555.1    Uniprot ID   -
Organism   Vibrio campbellii strain IFL1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 333775..345523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKR81_RS01590 (MKR81_01590) pilM 336074..337090 (+) 1017 WP_005432712.1 type IV pilus assembly protein PilM Machinery gene
  MKR81_RS01595 (MKR81_01595) pilN 337074..337649 (+) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  MKR81_RS01600 (MKR81_01600) pilO 337642..338235 (+) 594 WP_240304958.1 type 4a pilus biogenesis protein PilO Machinery gene
  MKR81_RS01605 (MKR81_01605) pilP 338225..338740 (+) 516 WP_005432702.1 pilus assembly protein PilP Machinery gene
  MKR81_RS01610 (MKR81_01610) pilQ 338775..340523 (+) 1749 WP_240305555.1 type IV pilus secretin PilQ family protein Machinery gene
  MKR81_RS01615 (MKR81_01615) aroK 340712..341230 (+) 519 WP_010443904.1 shikimate kinase AroK -
  MKR81_RS01620 (MKR81_01620) aroB 341262..342362 (+) 1101 WP_240304959.1 3-dehydroquinate synthase -
  MKR81_RS01625 (MKR81_01625) - 342416..343909 (+) 1494 WP_038889579.1 AAA family ATPase -
  MKR81_RS01630 (MKR81_01630) - 343979..344818 (+) 840 WP_038889578.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63748.71 Da        Isoelectric Point: 4.4873

>NTDB_id=659653 MKR81_RS01610 WP_240305555.1 338775..340523(+) (pilQ) [Vibrio campbellii strain IFL1]
MKMRHGLTLTPRKLTGLFSLWFFLLLAPFVAAEEVQSNSLKSIDFRTNKDKDAVIVVELASPAAIVDIKCVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAYIDEDYSHDYRLTGRYLEVKVGKLKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEKEIGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGDGEGDDGGSSVGIDDFLNVNLAATSPNA
TSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=659653 MKR81_RS01610 WP_240305555.1 338775..340523(+) (pilQ) [Vibrio campbellii strain IFL1]
ATGAAAATGAGACACGGATTAACACTGACACCAAGGAAGCTAACAGGGCTCTTCTCTTTATGGTTCTTTCTACTTTTAGC
CCCTTTCGTCGCAGCAGAAGAAGTACAGTCTAACTCGCTTAAGAGCATCGACTTCCGTACGAACAAAGACAAAGACGCCG
TGATTGTGGTTGAGCTCGCATCACCCGCTGCGATTGTTGATATTAAATGCGTACAAGAAGGCTTGAGTATCGACTTACTC
AACACCTCCGTTAAAGATGACCAGCTTTACCTACTTGATGTGAAAGATTTCTCGACGGTTGTCGAGAGCGTTGAGGTATT
CCGCGATACTTCTACAACACGTCTGGTGGCTTATATCGATGAGGACTATTCTCACGACTACCGTCTTACTGGTCGTTATT
TAGAGGTAAAGGTTGGCAAGCTTAAAGCCAACGAAAAAGCGCCGGATAAAAGCATTTTGGAAAAAGAAGGCAAGCTAATT
TCGATTAACTTCCAAGACATTCCTGTGCGAAACGTGTTGCAACTTATCGCCGACTACAATCAATTTAACTTGGTCGTCTC
TGACTCGGTTGAGGGTAACTTGACCTTGCGTTTGGATGGTGTGCCTTGGCAGCAAGTACTCGACATTATCTTGCAAGTGA
AAGGGTTAGATAAGCGTGTTGATGGCAATGTCATTCTCGTTGCACCAAAAGATGAATTGGATCTGCGCGAGAAGCAGCAA
CTCGAAAAGCAAAAGCTAGAGAAAGAGATCGGTGAGCTTTCATCAGAGATCATCAAGGTGAACTTCGCTAAAGCGTCAGA
CATTGCTGAGATGATCAATGGGGAGGGCAACATCAGTATGCTTTCAGAACGAGGCAGCATGACCATTGATGAGCGAACAA
ACTCGTTATTGATTCGTGAGTTACCTGAGAATATTGAAGTGATTCGCGAGATCATAGAATCATTGGATATTCCTGTAAAA
CAGGTTCAGATTGAAGCGCGTATTGTGACTATTAATGAAGGTAACATGGACGAACTTGGCGTTCGTTGGGGCTTCACCTC
CATTAATGGCAGTCATACTGTGGGTGGTTCAATTGAGAATAACCTTGCGACAATCGGCTTGTACGATGGCGGCGGTGACG
GTGAAGGTGATGACGGTGGCAGCAGTGTGGGTATTGACGACTTTCTCAACGTTAACCTTGCTGCGACCAGTCCAAATGCG
ACGAGTATTGCCTTCCAAGTTGCGAAGTTAGGTTCGGATACCTTACTAGATTTGGAACTTTCGGCGCTGCAACAAGAATC
AAAAGCAGAAATTATTTCCAGTCCACGTTTGATCACCACGAATAAGAAGCCAGCTTACATTGAGCAGGGTACCGAGATCC
CTTACCTTGAATCTTCTTCCAGTGGTGCGACTTCTGTGACGTTCAAAAAAGCGGTATTGAGCTTGAAAGTGACACCACAG
ATTACACCTGACAACCGTTTGGTGTTGGATTTGAGCGTTACGCAAGACAGACCGGGCCAAGTGGTAAAAACAGGCACTGG
TGAAGCGGTGGCGATCGATACGCAACGTATTGGTACACAAGTTTTAGTCAATAATGGCGAGACAGTCGTGTTGGGCGGTA
TTTTTCAACACAGCATTACAAACTCCGTCGATAAGGTGCCTTTATTAGGTGATTTGCCGCTGTTAGGTGCGCTTTTCCGT
CGAAGTTACGAAAATGTCGGTAAAAGTGAACTACTGATTTTTGTTACACCTAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(422-580)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

98.797

100

0.988
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.641

97.595

0.758
  pilQ Vibrio cholerae strain A1552

77.641

97.595

0.758
  pilQ Legionella pneumophila strain Lp02

41.561

94.674

0.393
  pilQ Legionella pneumophila strain ERS1305867

41.561

94.674

0.393
  pilQ Pseudomonas aeruginosa PAK

40.32

96.735

0.39
  pilQ Acinetobacter baumannii D1279779

37.271

100

0.385
  pilQ Acinetobacter baumannii strain A118

37.271

100

0.385
  comQ Acinetobacter baylyi ADP1

37.005

99.828

0.369