Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   MKR81_RS01600 Genome accession   NZ_CP092682
Coordinates   337642..338235 (+) Length   197 a.a.
NCBI ID   WP_240304958.1    Uniprot ID   -
Organism   Vibrio campbellii strain IFL1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 332642..343235
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKR81_RS01585 (MKR81_01585) - 333328..335895 (-) 2568 WP_038889585.1 PBP1A family penicillin-binding protein -
  MKR81_RS01590 (MKR81_01590) pilM 336074..337090 (+) 1017 WP_005432712.1 type IV pilus assembly protein PilM Machinery gene
  MKR81_RS01595 (MKR81_01595) pilN 337074..337649 (+) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  MKR81_RS01600 (MKR81_01600) pilO 337642..338235 (+) 594 WP_240304958.1 type 4a pilus biogenesis protein PilO Machinery gene
  MKR81_RS01605 (MKR81_01605) pilP 338225..338740 (+) 516 WP_005432702.1 pilus assembly protein PilP Machinery gene
  MKR81_RS01610 (MKR81_01610) pilQ 338775..340523 (+) 1749 WP_240305555.1 type IV pilus secretin PilQ family protein Machinery gene
  MKR81_RS01615 (MKR81_01615) aroK 340712..341230 (+) 519 WP_010443904.1 shikimate kinase AroK -
  MKR81_RS01620 (MKR81_01620) aroB 341262..342362 (+) 1101 WP_240304959.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 22990.44 Da        Isoelectric Point: 4.8135

>NTDB_id=659651 MKR81_RS01600 WP_240304958.1 337642..338235(+) (pilO) [Vibrio campbellii strain IFL1]
MVNWQDLEMDEIAEWPLVPQLVAILLMVVLIQGFGYWFYLKPEEARLNTLTQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGDKESEHFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFQDVTWQRVSQESETLHFRVRANTYQFKPEEKKDAK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=659651 MKR81_RS01600 WP_240304958.1 337642..338235(+) (pilO) [Vibrio campbellii strain IFL1]
ATGGTTAATTGGCAAGACCTAGAAATGGATGAAATCGCTGAATGGCCGCTGGTACCTCAGTTGGTGGCGATTCTTCTTAT
GGTGGTGTTGATCCAAGGTTTTGGTTACTGGTTTTATTTGAAGCCAGAGGAAGCTCGCCTTAATACTTTGACCCAAGAAG
AGCAGACTTTAAAAGTCGCGCTGAGGATCAAAGCCAATAAAGTCGCTGCACTGCCGCAGCTTCAGTCACAACTTGATGAA
CTGACCACCCGCTATGAACTGCTTTTGCAACAACTGCCGGCACAGAAAGAATTGGCGACCATGCTAGCGTCGGTTAATGA
ACTCGGCATCGATAACAAGCTGACATTTACGCGTATTGATTGGGGAGATAAAGAGTCAGAGCACTTTCTTTACCGTTTGC
CGCTCAATATTGAGCTAACGGGTGACTTCCATGACATCGGTCGTTTTTCACAGGCCATCGCCACCTTACCACGCATCATC
ACATTTCAAGATGTAACTTGGCAGCGAGTTAGTCAAGAGAGTGAAACCTTGCACTTCCGTGTAAGAGCAAACACTTACCA
ATTCAAACCAGAGGAAAAGAAAGATGCGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

98.947

96.447

0.954
  pilO Vibrio cholerae strain A1552

72.251

96.954

0.701