Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   MKR81_RS01590 Genome accession   NZ_CP092682
Coordinates   336074..337090 (+) Length   338 a.a.
NCBI ID   WP_005432712.1    Uniprot ID   -
Organism   Vibrio campbellii strain IFL1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 331074..342090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKR81_RS01575 (MKR81_01575) - 331439..332167 (-) 729 WP_005432732.1 redoxin family protein -
  MKR81_RS01580 (MKR81_01580) oxyR 332341..333249 (+) 909 WP_005432705.1 DNA-binding transcriptional regulator OxyR -
  MKR81_RS01585 (MKR81_01585) - 333328..335895 (-) 2568 WP_038889585.1 PBP1A family penicillin-binding protein -
  MKR81_RS01590 (MKR81_01590) pilM 336074..337090 (+) 1017 WP_005432712.1 type IV pilus assembly protein PilM Machinery gene
  MKR81_RS01595 (MKR81_01595) pilN 337074..337649 (+) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  MKR81_RS01600 (MKR81_01600) pilO 337642..338235 (+) 594 WP_240304958.1 type 4a pilus biogenesis protein PilO Machinery gene
  MKR81_RS01605 (MKR81_01605) pilP 338225..338740 (+) 516 WP_005432702.1 pilus assembly protein PilP Machinery gene
  MKR81_RS01610 (MKR81_01610) pilQ 338775..340523 (+) 1749 WP_240305555.1 type IV pilus secretin PilQ family protein Machinery gene
  MKR81_RS01615 (MKR81_01615) aroK 340712..341230 (+) 519 WP_010443904.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37860.29 Da        Isoelectric Point: 6.3141

>NTDB_id=659649 MKR81_RS01590 WP_005432712.1 336074..337090(+) (pilM) [Vibrio campbellii strain IFL1]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEMKKELPFRARKVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFIKVDTDEQMRGTDSYQVFATRKDVVETRVEALQKSGLKPV
LVDVHSQSLGHMWKLVAERFPEKNKYCLLDIGSLNSSFTMFTEQGELFHKEFPCGTRTTGSLFQQDPLADDAQVATEQFN
RQIVERVKRQMQLYTSINGSQDIKGVWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDNAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=659649 MKR81_RS01590 WP_005432712.1 336074..337090(+) (pilM) [Vibrio campbellii strain IFL1]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCACTACTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATGAAAAAAGAGCTACCTTTTCGTGCTCGAAAGGTCGCAATTTCAGTTCCAGATAATGCA
GTGATCAGTAAAAAGCTGCAGATAGAGCAGAACTTAGAAGAGAGTGAAAAGGAGTTTGCTGTTGTACAAGCCTTCTCACA
TCAATCTCCATTCCCCGTGGAAGAGCTGAGTCTTGACTTCATCAAGGTTGATACGGACGAGCAGATGCGAGGCACTGACA
GTTATCAGGTCTTCGCGACTCGTAAAGACGTGGTAGAAACCCGAGTTGAGGCATTGCAGAAATCAGGCTTGAAACCAGTA
CTGGTGGACGTGCACTCACAAAGTCTTGGTCATATGTGGAAATTGGTAGCGGAACGTTTTCCTGAAAAGAACAAGTATTG
TTTACTGGACATTGGTTCTTTGAACAGTTCTTTCACTATGTTCACTGAACAAGGTGAATTGTTTCATAAAGAATTTCCCT
GTGGTACTCGCACTACGGGCAGTTTGTTTCAACAAGATCCATTAGCAGACGATGCACAAGTGGCAACGGAGCAATTTAAT
CGACAAATCGTTGAGCGTGTTAAACGTCAGATGCAGCTCTATACCTCAATTAATGGAAGCCAGGATATCAAAGGCGTTTG
GCTTTCGGGAGAGGGCGCTGCAACACCTATGTTGGCCGAGGAGCTGTCTAGCCAGTTGGCACTAGAGTGTGAACTGCTTA
ATCCACTTGGTTTGTTTGAAATGAAGGTGTCTAAACGCAAACGTCGTGCCGCGGATTGGCAACATTTCACTGCTGCAGCC
GGACTGGCTGTACGTGGTATTCAGTGGCTAGGAGGTGACAATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

98.225

100

0.982
  pilM Vibrio cholerae strain A1552

49.415

100

0.5