Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SRAA_RS00440 Genome accession   NZ_AP014568
Coordinates   87065..88201 (-) Length   378 a.a.
NCBI ID   WP_045530255.1    Uniprot ID   A0A060NKI0
Organism   Serpentinimonas raichei strain A1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 82065..93201
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SRAA_RS00410 (SRAA_0083) - 82881..83501 (-) 621 WP_045530240.1 DUF502 domain-containing protein -
  SRAA_RS00415 (SRAA_0084) - 83641..84012 (+) 372 WP_045530245.1 hypothetical protein -
  SRAA_RS00420 (SRAA_0085) rsmI 84022..84951 (-) 930 WP_045530247.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  SRAA_RS00425 (SRAA_0086) - 84950..85405 (+) 456 WP_045530249.1 YraN family protein -
  SRAA_RS00430 (SRAA_0087) - 85475..86143 (+) 669 WP_045530251.1 BON domain-containing protein -
  SRAA_RS00435 (SRAA_0088) - 86150..87052 (-) 903 WP_045530253.1 NAD(P)-dependent oxidoreductase -
  SRAA_RS00440 (SRAA_0089) pilU 87065..88201 (-) 1137 WP_045530255.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SRAA_RS00445 (SRAA_0090) pilT 88236..89279 (-) 1044 WP_045530257.1 type IV pilus twitching motility protein PilT Machinery gene
  SRAA_RS00450 (SRAA_0091) - 89313..90032 (+) 720 WP_045530259.1 YggS family pyridoxal phosphate-dependent enzyme -
  SRAA_RS11970 (SRAA_0092) - 90008..91462 (-) 1455 WP_052467419.1 PAS domain-containing sensor histidine kinase -
  SRAA_RS00460 (SRAA_0093) - 91537..91950 (-) 414 WP_034109967.1 helix-turn-helix domain-containing protein -
  SRAA_RS00465 (SRAA_0094) - 91978..92271 (-) 294 WP_045530262.1 type II toxin-antitoxin system HigB family toxin -
  SRAA_RS00470 (SRAA_0095) hepT 92443..92865 (-) 423 WP_034109969.1 type VII toxin-antitoxin system HepT family RNase toxin -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42048.23 Da        Isoelectric Point: 7.0408

>NTDB_id=65921 SRAA_RS00440 WP_045530255.1 87065..88201(-) (pilU) [Serpentinimonas raichei strain A1]
MERDQASQFVNDLLRLMVSRSGSDLFLTADFPPAIKVDGKITKVSPQPLSGQHTQALARAIMNDKQSAEFERSMECNFAI
SPGGIGRFRVNAFVQMGKVGLVMRTIPAKIPTIDELGLPQVLKNLALAKRGICILVGGTGSGKSTTLAAMVDWRNEHTHD
HIVTIEDPVEFVHQHKNCIVTQREVGLDTDDWGIALKNSLRQAPNVILMGEIRDRETMQHAISFSETGHLCLATLHANSA
NQAMERIINFFPDEVHMQILKDVSLNVRGMVAQRLLPRQDGKGRHAVVEILLHSGLIADQIMKGEINELKETMRKSRESG
MQTFDQSLFDAFEANLISFEDAIRNADSQNDLKLRIKLHSQRARNTDLAAGTEHLHIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=65921 SRAA_RS00440 WP_045530255.1 87065..88201(-) (pilU) [Serpentinimonas raichei strain A1]
ATGGAACGCGATCAAGCCAGCCAATTTGTCAACGACCTGCTGCGGCTCATGGTGAGCCGCAGCGGCAGCGACTTGTTTCT
CACCGCCGACTTCCCGCCCGCGATCAAGGTCGATGGCAAGATCACCAAGGTCTCGCCGCAGCCGCTCAGCGGACAGCACA
CGCAGGCGCTGGCGCGCGCCATCATGAACGACAAGCAGTCGGCCGAGTTCGAGCGCAGCATGGAGTGCAACTTCGCCATC
TCGCCCGGTGGCATCGGGCGCTTTCGCGTCAACGCCTTTGTGCAAATGGGCAAGGTGGGGCTGGTGATGCGCACCATTCC
GGCCAAAATTCCGACCATCGACGAGCTGGGTCTGCCGCAGGTGCTCAAGAACCTGGCCTTGGCCAAGCGCGGCATCTGCA
TCCTGGTGGGCGGCACCGGTTCCGGCAAATCGACCACGCTGGCGGCCATGGTGGACTGGCGCAACGAGCACACGCACGAC
CACATCGTGACCATCGAAGACCCGGTGGAGTTTGTGCACCAGCACAAGAACTGCATCGTGACCCAGCGCGAGGTGGGGCT
GGACACCGACGACTGGGGCATTGCCCTGAAAAACTCGCTGCGCCAGGCCCCGAACGTGATTTTGATGGGCGAAATCCGCG
ACCGGGAAACTATGCAGCACGCCATTTCCTTCTCGGAAACCGGGCATTTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGATGGAGCGCATCATCAACTTCTTCCCCGACGAAGTGCACATGCAGATCCTCAAAGACGTGTCGCTCAACGT
GCGCGGCATGGTGGCGCAGCGTCTGCTGCCGCGCCAAGACGGCAAGGGCCGCCACGCGGTGGTGGAAATCCTGCTGCACA
GTGGCTTGATCGCGGACCAGATCATGAAGGGCGAGATCAATGAGCTCAAAGAGACCATGCGCAAGAGCCGCGAGAGCGGG
ATGCAGACCTTCGATCAATCGTTGTTCGACGCCTTCGAGGCCAACCTGATCAGCTTCGAGGACGCCATCCGCAACGCCGA
CTCGCAAAACGACCTCAAGCTGCGCATCAAGCTGCACAGCCAGCGCGCCCGCAACACCGACCTCGCAGCGGGCACCGAAC
ACTTGCACATCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(86-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A060NKI0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.453

92.328

0.54
  pilU Vibrio cholerae strain A1552

53.168

96.032

0.511
  pilU Acinetobacter baylyi ADP1

52.793

94.709

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.323

89.153

0.386
  pilT Acinetobacter baumannii D1279779

40.882

89.947

0.368
  pilT Acinetobacter baumannii strain A118

40.882

89.947

0.368
  pilT Acinetobacter nosocomialis M2

40.882

89.947

0.368
  pilT Legionella pneumophila strain ERS1305867

41.945

87.037

0.365
  pilT Legionella pneumophila strain Lp02

41.945

87.037

0.365
  pilT Pseudomonas aeruginosa PAK

40.653

89.153

0.362

Multiple sequence alignment