Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MJO52_RS15860 Genome accession   NZ_CP092418
Coordinates   3574227..3575444 (+) Length   405 a.a.
NCBI ID   WP_252082949.1    Uniprot ID   -
Organism   Microbulbifer variabilis strain SCSIO 43006     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3569227..3580444
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJO52_RS15840 (MJO52_15830) imuA 3569505..3570212 (+) 708 WP_252082946.1 translesion DNA synthesis-associated protein ImuA -
  MJO52_RS15845 (MJO52_15835) - 3570234..3571214 (+) 981 WP_252082947.1 DNA polymerase Y family protein -
  MJO52_RS21325 - 3571558..3571980 (-) 423 WP_286036973.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MJO52_RS15855 (MJO52_15845) pilB 3572360..3574072 (+) 1713 WP_252082948.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MJO52_RS15860 (MJO52_15850) pilC 3574227..3575444 (+) 1218 WP_252082949.1 type II secretion system F family protein Machinery gene
  MJO52_RS15865 (MJO52_15855) pilD 3575457..3576335 (+) 879 WP_252082950.1 A24 family peptidase Machinery gene
  MJO52_RS15870 (MJO52_15860) coaE 3576470..3577066 (+) 597 WP_252082951.1 dephospho-CoA kinase -
  MJO52_RS15875 (MJO52_15865) yacG 3577140..3577355 (+) 216 WP_252082952.1 DNA gyrase inhibitor YacG -
  MJO52_RS15880 (MJO52_15870) mutT 3577421..3577819 (-) 399 WP_252082953.1 8-oxo-dGTP diphosphatase MutT -
  MJO52_RS15885 (MJO52_15875) argJ 3577829..3579031 (-) 1203 WP_252082954.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43683.43 Da        Isoelectric Point: 9.6629

>NTDB_id=657334 MJO52_RS15860 WP_252082949.1 3574227..3575444(+) (pilC) [Microbulbifer variabilis strain SCSIO 43006]
MANAAAVAYIYKGVDSKGAKVQGEISGTSPALVKAQLRKQGIIANRVQKKPKPLFGGGKKKVKPADIALFTRQLATMMKA
GVPLVQSFEIVADGLDNEGVRELILKIRDDVASGTAFADALRKHPLFFDDLFCNLVASGEQSGALEVMLDRIATYKEKTE
ALKAKIKKAMTYPLAVIVVAVIVTSILLIKVVPQFADTFESFGAELPAFTQFVVGISEWMQAYWFIALIGTTLTVGGILE
AKKRNKTVDRFFGKLALKLPIIGKITYNSIAARFARTLSTTFAAGVPLIDALRSVAGATGNYIYEEATLKIRDSVATGIP
LNAAMRQSGLYPVMLVQMTSIGEESGALDEMLSKAATFYEEAVDNDVDNLTTLLEPMIMSILGVLVGGLLIAMYLPIFQM
GQVIK

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=657334 MJO52_RS15860 WP_252082949.1 3574227..3575444(+) (pilC) [Microbulbifer variabilis strain SCSIO 43006]
ATGGCCAATGCCGCAGCAGTCGCATATATCTATAAAGGTGTAGATAGCAAAGGGGCTAAGGTTCAAGGTGAGATAAGCGG
TACCAGCCCCGCACTAGTTAAGGCGCAACTGCGTAAGCAGGGCATTATAGCCAACCGTGTACAGAAAAAGCCCAAACCAT
TATTTGGTGGTGGCAAGAAGAAGGTAAAACCTGCAGATATCGCCCTATTTACTCGGCAGCTAGCCACAATGATGAAAGCA
GGGGTGCCTCTTGTACAAAGCTTTGAGATCGTTGCCGATGGCTTAGACAACGAAGGGGTTAGGGAACTAATTTTAAAGAT
CCGTGACGATGTGGCCTCTGGTACCGCATTCGCTGATGCCCTTCGTAAGCATCCTCTCTTTTTTGATGATTTATTTTGCA
ACTTAGTCGCCTCAGGAGAGCAATCGGGCGCCTTAGAAGTGATGCTCGACCGCATCGCCACGTATAAGGAAAAGACCGAA
GCCCTAAAAGCAAAAATTAAAAAAGCGATGACTTATCCGCTAGCGGTTATCGTCGTAGCAGTTATTGTTACCTCTATACT
TCTTATCAAGGTCGTACCACAATTTGCAGATACTTTTGAAAGTTTCGGAGCAGAGCTCCCTGCATTTACCCAGTTTGTAG
TTGGTATTTCTGAGTGGATGCAGGCTTATTGGTTCATTGCCTTAATTGGCACTACCCTTACAGTTGGCGGCATCCTCGAA
GCAAAAAAGAGAAATAAAACAGTTGATCGTTTTTTTGGAAAGCTAGCTTTAAAGCTACCAATTATTGGCAAAATAACTTA
CAACTCTATTGCTGCTCGTTTTGCTCGAACTCTTTCTACAACCTTTGCAGCCGGTGTCCCCCTCATCGACGCATTGCGAT
CAGTAGCTGGGGCAACAGGCAATTATATTTATGAAGAGGCAACACTCAAAATTCGTGACTCTGTTGCTACGGGCATCCCA
CTAAATGCCGCCATGCGCCAGTCCGGACTATATCCTGTAATGCTTGTCCAAATGACATCCATCGGCGAAGAATCAGGAGC
GCTAGATGAAATGCTGAGTAAGGCCGCCACTTTCTATGAAGAAGCAGTAGACAATGATGTAGATAACTTAACCACACTTC
TTGAACCAATGATTATGTCTATCCTCGGGGTACTGGTCGGGGGGCTATTGATAGCCATGTACCTACCTATATTCCAAATG
GGCCAAGTAATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(70-193)

Type II secretion system (T2SS), protein F (T2SSF)

(274-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

64.141

97.778

0.627
  pilC Acinetobacter baumannii D1279779

62.217

98.025

0.61
  pilC Acinetobacter baylyi ADP1

60.957

98.025

0.598
  pilC Legionella pneumophila strain ERS1305867

55.025

98.272

0.541
  pilG Neisseria gonorrhoeae MS11

44.584

98.025

0.437
  pilG Neisseria meningitidis 44/76-A

44.332

98.025

0.435
  pilC Vibrio cholerae strain A1552

41.667

97.778

0.407
  pilC Vibrio campbellii strain DS40M4

39.295

98.025

0.385
  pilC Thermus thermophilus HB27

35.885

100

0.37