Detailed information NTG0657333

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: MJO52_RS15855
• Genome accession: NZ_CP092418
• Coordinates: 3572360..3574072 (+)
• Length: 570 a.a.
• NCBI ID: WP_252082948.1
• Uniprot ID: -
• Organism: Microbulbifer variabilis strain SCSIO 43006
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3567360..3579072

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MJO52_RS15830 (MJO52_15820)	-	3567493..3568701 (-)	1209	WP_252082944.1	NAD(P)/FAD-dependent oxidoreductase	-
MJO52_RS15835 (MJO52_15825)	dcd	3568730..3569299 (-)	570	WP_252082945.1	dCTP deaminase	-
MJO52_RS15840 (MJO52_15830)	imuA	3569505..3570212 (+)	708	WP_252082946.1	translesion DNA synthesis-associated protein ImuA	-
MJO52_RS15845 (MJO52_15835)	-	3570234..3571214 (+)	981	WP_252082947.1	DNA polymerase Y family protein	-
MJO52_RS21325	-	3571558..3571980 (-)	423	WP_286036973.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
MJO52_RS15855 (MJO52_15845)	pilB	3572360..3574072 (+)	1713	WP_252082948.1	type IV-A pilus assembly ATPase PilB	Machinery gene
MJO52_RS15860 (MJO52_15850)	pilC	3574227..3575444 (+)	1218	WP_252082949.1	type II secretion system F family protein	Machinery gene
MJO52_RS15865 (MJO52_15855)	pilD	3575457..3576335 (+)	879	WP_252082950.1	A24 family peptidase	Machinery gene
MJO52_RS15870 (MJO52_15860)	coaE	3576470..3577066 (+)	597	WP_252082951.1	dephospho-CoA kinase	-
MJO52_RS15875 (MJO52_15865)	yacG	3577140..3577355 (+)	216	WP_252082952.1	DNA gyrase inhibitor YacG	-
MJO52_RS15880 (MJO52_15870)	mutT	3577421..3577819 (-)	399	WP_252082953.1	8-oxo-dGTP diphosphatase MutT	-
MJO52_RS15885 (MJO52_15875)	argJ	3577829..3579031 (-)	1203	WP_252082954.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-

Sequence

Protein

• Length: 570 a.a.
• Molecular weight: 62244.02 Da
• Isoelectric Point: 5.4620

>NTDB_id=657333 MJO52_RS15855 WP_252082948.1 3572360..3574072(+) (pilB) [Microbulbifer variabilis strain SCSIO 43006]
MTSPSLNGLAKRLVADGILDEATAISALKAAHRSGQSFAQHAVEAKLVKSRELANIASQAFGTPLFDFGSYNFEMIPKGI
IDEKLISKHFTLPLYRRGNRLFIAVADPTNLAGLDEINFNTGLSTDAVLVETDKLGKAIESYLSDSQDIGGGLKGIDDEE
LDSLNVGESESESDDESEPGGDEAPVVRFVNKVLLDAIRTGASDIHFEPYEKSYRVRFRTDGVLHEIARPPIQLATRLSA
RLKVMSKMDISERRVPQDGRIKMKLSKTKAIDFRVNSLPTLWGEKIVLRILDPSSAKMGIDALGYEEEQKKIYMDALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTPELNISTAEDPVEINLEGINQVNVSSKVGLNFAEALRSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNIATSVSVIIAQRLARRLCNECKKATTLPSEVLEAEGF
SAVNIPRNDWAIYQPVGCEHCSKGYKGRVGVYEVVRITDGISRIIMEGGNSIQIADQARKEGFNNLRTSALRKVVMGITS
LEEANRVTKD

Nucleotide

• Length: 1713 bp

>NTDB_id=657333 MJO52_RS15855 WP_252082948.1 3572360..3574072(+) (pilB) [Microbulbifer variabilis strain SCSIO 43006]
ATGACTAGCCCCTCCCTTAACGGCCTGGCAAAACGCTTGGTAGCAGATGGAATTCTCGACGAAGCCACAGCAATTTCTGC
ACTTAAGGCTGCCCATCGTTCAGGCCAGAGTTTCGCCCAACATGCGGTAGAAGCCAAGCTAGTAAAAAGCCGGGAGCTCG
CCAATATTGCTTCCCAAGCCTTCGGCACACCGTTATTTGATTTTGGCAGTTACAATTTCGAGATGATCCCCAAGGGTATC
ATCGACGAAAAATTAATCAGCAAACATTTTACGCTCCCCCTCTATAGGCGTGGTAATCGCTTATTTATCGCTGTCGCAGA
CCCCACCAACCTGGCAGGGTTGGACGAGATCAATTTTAATACTGGCCTCAGTACTGATGCGGTTTTAGTAGAAACCGATA
AGCTGGGAAAGGCTATTGAAAGCTACCTCTCTGATAGCCAAGATATCGGGGGAGGCCTGAAGGGTATCGATGATGAAGAG
CTAGACTCCCTCAATGTAGGCGAAAGCGAATCTGAATCCGACGATGAAAGCGAACCGGGTGGGGATGAAGCGCCCGTTGT
AAGGTTTGTCAATAAGGTTCTTCTAGATGCAATTCGCACTGGTGCCTCCGATATTCACTTTGAACCCTACGAAAAATCTT
ATCGGGTTCGTTTTCGCACCGATGGCGTATTGCATGAAATTGCCAGGCCTCCGATTCAGTTAGCAACACGCCTATCTGCC
CGCTTGAAAGTGATGTCGAAGATGGATATCTCGGAACGGCGAGTGCCCCAGGACGGCCGGATTAAAATGAAGCTGTCCAA
GACCAAGGCTATCGACTTCCGGGTAAACAGTTTGCCAACCCTATGGGGAGAAAAGATTGTTTTACGGATTCTCGACCCCT
CTTCAGCAAAAATGGGAATTGATGCCCTCGGCTATGAAGAGGAACAAAAAAAGATCTACATGGATGCCCTGGCACAACCC
CAAGGGATGATCCTGGTTACCGGCCCCACTGGTTCAGGTAAAACAGTTTCTCTATACACCGGCCTCAATATTCTCAACAC
TCCTGAGCTCAACATTTCTACCGCTGAAGACCCGGTAGAAATCAACCTGGAAGGGATCAATCAGGTTAATGTCTCCAGCA
AAGTTGGGCTAAATTTCGCTGAAGCCCTGCGCTCTTTCCTGCGACAAGACCCTGATATCGTCATGGTAGGAGAGATTAGG
GATTTGGAAACCGCAGAAATTGCTATCAAAGCAGCGCAAACTGGGCACCTGGTACTCTCAACCCTGCATACCAACTCAGC
TCCAGAAACCCTTACGCGCCTGATGAATATGGGGGTTCCCACTTTTAATATTGCGACTTCCGTCAGTGTTATTATCGCCC
AGCGCTTGGCGAGGCGTTTGTGTAATGAATGCAAAAAGGCTACTACTCTTCCCAGTGAGGTCTTGGAAGCCGAGGGTTTC
TCTGCAGTGAATATTCCAAGGAACGACTGGGCCATTTACCAGCCTGTTGGCTGCGAGCACTGCTCAAAAGGCTACAAGGG
CCGCGTGGGTGTGTATGAAGTGGTTCGCATCACTGATGGCATCTCCAGAATTATAATGGAGGGCGGCAATTCCATTCAGA
TCGCCGATCAGGCGCGCAAAGAGGGTTTTAATAATCTAAGGACTTCTGCATTGCGCAAAGTGGTAATGGGCATTACCAGC
CTGGAGGAAGCCAACCGAGTAACCAAAGATTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (13-145)

• Type II/IV secretion system protein (T2SSE) (192-459)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	60.702	100	0.607
pilB	Acinetobacter baylyi ADP1	58.947	100	0.589
pilB	Legionella pneumophila strain ERS1305867	54.145	99.474	0.539
pilB	Vibrio campbellii strain DS40M4	52.021	99.825	0.519
pilB	Vibrio parahaemolyticus RIMD 2210633	52.135	98.596	0.514
pilB	Vibrio cholerae strain A1552	51.878	98.07	0.509
pilF	Neisseria gonorrhoeae MS11	47.95	98.421	0.472
pilB/pilB1	Synechocystis sp. PCC 6803	38.399	100	0.412