Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MID13_RS14245 Genome accession   NZ_CP092384
Coordinates   3152681..3154375 (-) Length   564 a.a.
NCBI ID   WP_239718124.1    Uniprot ID   -
Organism   Vibrio gigantis strain ACE001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3147681..3159375
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MID13_RS14225 (MID13_14225) - 3148360..3149205 (-) 846 WP_239716778.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  MID13_RS14230 (MID13_14230) - 3149275..3150792 (-) 1518 WP_239716780.1 SPOR domain-containing protein -
  MID13_RS14235 (MID13_14235) aroB 3150872..3151960 (-) 1089 WP_215406522.1 3-dehydroquinate synthase -
  MID13_RS14240 (MID13_14240) aroK 3151986..3152504 (-) 519 WP_017061125.1 shikimate kinase AroK -
  MID13_RS14245 (MID13_14245) pilQ 3152681..3154375 (-) 1695 WP_239718124.1 type IV pilus secretin PilQ Machinery gene
  MID13_RS14250 (MID13_14250) pilP 3154435..3154953 (-) 519 WP_086715781.1 pilus assembly protein PilP Machinery gene
  MID13_RS14255 (MID13_14255) pilO 3154940..3155551 (-) 612 WP_086715787.1 type 4a pilus biogenesis protein PilO Machinery gene
  MID13_RS14260 (MID13_14260) pilN 3155544..3156143 (-) 600 WP_215406528.1 PilN domain-containing protein Machinery gene
  MID13_RS14265 (MID13_14265) pilM 3156127..3157152 (-) 1026 WP_239716782.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 61433.98 Da        Isoelectric Point: 4.3431

>NTDB_id=657102 MID13_RS14245 WP_239718124.1 3152681..3154375(-) (pilQ) [Vibrio gigantis strain ACE001]
MNKGLQGLIVSVILVFSMLSHAESLPNKLENIDFRVNKDKDAVIIIELATSTAVVDVQRAQEGLSIELINTQVDDDKLYL
LDVKDFATLVEGIEVYKETPSTRLLVTIASDYQYEYNLKDRFIEVVISKPVIDEASKEKSILEKEGKLISINFQDIPVRN
VLQLIADYNDFNLVVSDSVAGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKAELDLREQQALEKSRLEEELG
ELKSEIIKINFAKATDIADMIGGEGSVSMLSDRGSITIDERTNSLLIRELEENIAVIRGIIESLDIPVKQVQIEARIVTV
TEGNLDELGVRWGVSSTNGSFTVGGSIEGNHPSQITPYDDDGGNSAIDDYLNVNLGATSPNASSIAFQVAKLGSDTLLDL
ELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLVLDLSVTQDRP
GQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSVSSTVDKVPLLGDLPVLGALFRRSYENVGKSELLIFVTPK
VVIQ

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=657102 MID13_RS14245 WP_239718124.1 3152681..3154375(-) (pilQ) [Vibrio gigantis strain ACE001]
ATGAACAAAGGGCTGCAAGGCTTAATTGTGTCTGTGATATTGGTTTTTAGCATGCTCAGCCATGCCGAGAGCTTACCTAA
TAAATTAGAGAATATTGATTTTAGGGTAAACAAGGATAAAGACGCTGTCATCATCATTGAGCTAGCGACGAGCACCGCTG
TGGTGGATGTTCAACGTGCTCAGGAAGGCTTAAGTATCGAGTTGATCAATACTCAGGTTGATGACGACAAGCTCTATCTT
CTGGATGTGAAAGACTTCGCTACCTTAGTTGAAGGCATCGAAGTCTATAAAGAGACGCCAAGCACACGACTGTTGGTGAC
AATCGCGAGTGACTATCAGTATGAATACAACCTAAAAGATCGTTTTATTGAGGTCGTGATCAGCAAACCTGTTATCGATG
AAGCGTCTAAAGAAAAAAGCATTCTAGAGAAAGAGGGCAAGTTGATATCGATTAACTTTCAAGATATTCCAGTACGGAAC
GTCCTGCAGTTGATTGCCGACTATAACGATTTCAATCTCGTCGTGTCTGATTCGGTAGCCGGAAACCTGACGTTACGCTT
AGATGGCGTACCTTGGCAGCAAGTTCTCGACATTATCTTGCAAGTTAAAGGCTTGGATAAGCGTGTTGATGGTAATGTCA
TCCTGGTTGCGCCGAAAGCGGAGCTTGATCTTCGAGAGCAACAAGCGTTAGAGAAGTCTCGTTTGGAAGAGGAGTTAGGG
GAGCTTAAGTCGGAAATCATCAAGATTAACTTTGCTAAAGCCACCGACATTGCCGATATGATTGGCGGTGAAGGCTCGGT
AAGCATGTTGTCTGATCGCGGTTCGATTACCATTGATGAGCGCACTAACTCTTTGTTGATTCGTGAGTTGGAGGAGAACA
TTGCCGTGATACGAGGCATCATTGAATCCTTAGATATTCCCGTGAAACAGGTGCAGATAGAGGCGCGTATCGTGACCGTC
ACTGAGGGTAATCTTGATGAGCTTGGTGTGCGCTGGGGTGTTTCCTCGACCAACGGAAGCTTCACCGTTGGCGGTTCAAT
AGAAGGTAATCATCCATCACAAATCACACCGTATGACGATGATGGTGGCAACAGTGCGATTGATGACTACCTCAACGTTA
ACTTGGGGGCGACATCGCCGAATGCATCGAGCATTGCTTTTCAGGTCGCCAAGCTTGGTTCGGATACTTTGCTCGACCTT
GAACTGTCGGCACTGCAACAAGAGTCGAAAGCTGAGATTATTTCTAGCCCACGTTTAATCACCACCAATAAAAAGCCCGC
TTATATTGAGCAAGGTACTGAAATTCCTTACTTAGAGTCTTCTTCAAGTGGTGCAACGTCGGTCGCATTTAAGAAAGCGG
TATTGAGCCTCAAGGTGACTCCGCAGATAACCCCTGACAATCGTTTGGTATTGGATTTGAGCGTGACTCAAGATAGACCA
GGGCAAGTGGTCAAAACCGGAACGGGTGAAGCAGTAGCAATTGACACGCAAAGAATAGGTACGCAAGTGCTTGTTAATAA
TGGTGAAACGGTTGTTCTTGGCGGGATATTTCAACACAGCGTAAGCAGCACGGTGGATAAAGTTCCTCTGTTGGGAGACC
TGCCAGTTTTGGGTGCATTGTTCCGTCGCAGTTATGAAAATGTGGGTAAAAGTGAACTACTTATTTTTGTCACACCCAAA
GTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(245-310)

Bacterial type II and III secretion system protein (Secretin)

(404-562)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

80.328

97.34

0.782
  pilQ Vibrio cholerae strain A1552

80.328

97.34

0.782
  pilQ Vibrio campbellii strain DS40M4

80.331

96.454

0.775
  pilQ Legionella pneumophila strain ERS1305867

42.674

96.809

0.413
  pilQ Legionella pneumophila strain Lp02

42.674

96.809

0.413
  pilQ Pseudomonas aeruginosa PAK

41.441

98.404

0.408
  comQ Acinetobacter baylyi ADP1

39.223

100

0.394
  pilQ Acinetobacter baumannii D1279779

37.651

100

0.387
  pilQ Acinetobacter baumannii strain A118

37.651

100

0.387