Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   MID13_RS14255 Genome accession   NZ_CP092384
Coordinates   3154940..3155551 (-) Length   203 a.a.
NCBI ID   WP_086715787.1    Uniprot ID   A0A5M9NBH1
Organism   Vibrio gigantis strain ACE001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3149940..3160551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MID13_RS14235 (MID13_14235) aroB 3150872..3151960 (-) 1089 WP_215406522.1 3-dehydroquinate synthase -
  MID13_RS14240 (MID13_14240) aroK 3151986..3152504 (-) 519 WP_017061125.1 shikimate kinase AroK -
  MID13_RS14245 (MID13_14245) pilQ 3152681..3154375 (-) 1695 WP_239718124.1 type IV pilus secretin PilQ Machinery gene
  MID13_RS14250 (MID13_14250) pilP 3154435..3154953 (-) 519 WP_086715781.1 pilus assembly protein PilP Machinery gene
  MID13_RS14255 (MID13_14255) pilO 3154940..3155551 (-) 612 WP_086715787.1 type 4a pilus biogenesis protein PilO Machinery gene
  MID13_RS14260 (MID13_14260) pilN 3155544..3156143 (-) 600 WP_215406528.1 PilN domain-containing protein Machinery gene
  MID13_RS14265 (MID13_14265) pilM 3156127..3157152 (-) 1026 WP_239716782.1 type IV pilus assembly protein PilM Machinery gene
  MID13_RS14270 (MID13_14270) - 3157304..3159862 (+) 2559 WP_239716784.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 203 a.a.        Molecular weight: 23573.00 Da        Isoelectric Point: 4.4907

>NTDB_id=657104 MID13_RS14255 WP_086715787.1 3154940..3155551(-) (pilO) [Vibrio gigantis strain ACE001]
MANLQNRMSFQDLDVDEITEWPLLPQLLVILVLVVLIQGIGTWLYVLPLDDELQQMKQQEQTLKTTLRIKANKVAALPKL
QSQLDELTSRYDYLLEQLPVQKELASMLASVNELGLDNSLTFTRIDWGQKQNKEFLYRLPLNIELTGDYHEIGDFSAAIA
KLPRIISFDDVNWQRVSQESSTLHFRVRAYTYQFKSEVNDEIQ

Nucleotide


Download         Length: 612 bp        

>NTDB_id=657104 MID13_RS14255 WP_086715787.1 3154940..3155551(-) (pilO) [Vibrio gigantis strain ACE001]
ATGGCTAACCTTCAAAACAGGATGAGTTTTCAGGATCTTGATGTTGACGAGATCACCGAATGGCCGTTGCTGCCTCAGCT
CCTAGTCATTCTGGTGTTGGTAGTATTAATCCAAGGTATCGGAACCTGGCTTTATGTGCTGCCGCTCGACGACGAACTGC
AACAGATGAAGCAGCAAGAGCAGACCTTAAAAACGACCTTGCGAATAAAGGCCAACAAGGTTGCGGCTCTGCCTAAGTTA
CAGAGCCAGCTGGATGAGCTAACCAGTCGCTACGATTACCTGTTAGAGCAGCTCCCTGTTCAAAAAGAGCTAGCCAGTAT
GTTGGCGTCAGTGAATGAACTCGGCTTGGATAATTCGCTGACTTTCACTCGAATCGATTGGGGACAAAAGCAGAACAAGG
AGTTTCTCTATCGTTTACCGCTCAATATCGAGTTGACAGGTGACTACCATGAGATAGGCGACTTCTCTGCGGCTATCGCC
AAGCTGCCGCGTATCATCAGCTTTGATGATGTGAATTGGCAACGAGTTAGTCAAGAAAGCAGCACGCTGCACTTTAGGGT
TCGAGCTTATACCTACCAGTTTAAGTCGGAGGTTAATGATGAAATCCAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(53-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5M9NBH1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

75.648

95.074

0.719
  pilO Vibrio campbellii strain DS40M4

72.34

92.611

0.67