Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   MID13_RS14265 Genome accession   NZ_CP092384
Coordinates   3156127..3157152 (-) Length   341 a.a.
NCBI ID   WP_239716782.1    Uniprot ID   -
Organism   Vibrio gigantis strain ACE001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3151127..3162152
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MID13_RS14240 (MID13_14240) aroK 3151986..3152504 (-) 519 WP_017061125.1 shikimate kinase AroK -
  MID13_RS14245 (MID13_14245) pilQ 3152681..3154375 (-) 1695 WP_239718124.1 type IV pilus secretin PilQ Machinery gene
  MID13_RS14250 (MID13_14250) pilP 3154435..3154953 (-) 519 WP_086715781.1 pilus assembly protein PilP Machinery gene
  MID13_RS14255 (MID13_14255) pilO 3154940..3155551 (-) 612 WP_086715787.1 type 4a pilus biogenesis protein PilO Machinery gene
  MID13_RS14260 (MID13_14260) pilN 3155544..3156143 (-) 600 WP_215406528.1 PilN domain-containing protein Machinery gene
  MID13_RS14265 (MID13_14265) pilM 3156127..3157152 (-) 1026 WP_239716782.1 type IV pilus assembly protein PilM Machinery gene
  MID13_RS14270 (MID13_14270) - 3157304..3159862 (+) 2559 WP_239716784.1 penicillin-binding protein 1A -
  MID13_RS14275 (MID13_14275) oxyR 3159956..3160849 (-) 894 WP_004737158.1 DNA-binding transcriptional regulator OxyR -
  MID13_RS14280 (MID13_14280) - 3161071..3161799 (+) 729 WP_048659171.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38388.86 Da        Isoelectric Point: 6.5757

>NTDB_id=657106 MID13_RS14265 WP_239716782.1 3156127..3157152(-) (pilM) [Vibrio gigantis strain ACE001]
MGSSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKELPFGCRNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQIYATRKEVVDSRADALTKAGFKP
VVVDTQAHGLLNIWQLASRLYPEKKNWLLVDVGIDQTSLGIIPQGSVPFYKDIGFGTKDLRSHDDPSDMECAFGNTEDTH
QFIVNLIEKLKRQLQLYSSGHAQQSISGIWLMGEGASIPMVTEELERHFQMSCESLNPLSLFENKVAKRRRLSMDWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=657106 MID13_RS14265 WP_239716782.1 3156127..3157152(-) (pilM) [Vibrio gigantis strain ACE001]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGCATTAAAGCCGTGGTACTAAAACCCGTGGGGGAGTT
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTTAAAGAACTTAGGAAAGAACTGCCTTTTGGCTGTCGCAACGTCGCCATTTCGGTACCTGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGTGAGTTAGAAGACAGAGAAAAAGAGTTTTCGATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCTATTGAGGAGCTGAGTTTAGATTTTGTAAAGCTGGAAGACAAACGATTTGGCAAAGGTTCGACAA
GCAGTTATCAAATATACGCAACTCGTAAGGAAGTGGTGGATAGCCGAGCGGATGCATTAACTAAGGCTGGCTTTAAGCCT
GTTGTGGTGGATACGCAAGCGCATGGGTTACTTAATATCTGGCAATTGGCCTCGCGCCTGTATCCTGAAAAGAAAAACTG
GCTGCTGGTGGATGTTGGAATCGATCAAACGTCGCTAGGTATTATTCCACAAGGTTCGGTACCGTTTTACAAAGACATCG
GCTTTGGTACCAAAGATCTTCGTAGTCATGACGACCCTAGTGATATGGAGTGTGCTTTCGGCAACACGGAAGACACACAT
CAATTCATTGTTAACCTGATTGAGAAGCTCAAACGTCAATTGCAGCTCTATTCTTCGGGACATGCGCAGCAATCTATCTC
TGGGATCTGGCTGATGGGTGAAGGTGCCAGCATCCCTATGGTAACCGAAGAGTTAGAACGTCATTTTCAGATGAGCTGCG
AGTCATTGAATCCTTTGTCGTTGTTTGAGAATAAGGTTGCCAAGAGACGCCGACTGTCGATGGACTGGCAACACTTTGGT
ATTGCGGCAGGTATGGCGATGAGTGGACTCAAGTGGCAGGGAGAGAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(242-328)

Type IV pilus assembly protein PilM; (PilM_2)

(8-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

54.252

100

0.543
  pilM Vibrio campbellii strain DS40M4

48.406

100

0.49