Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MID13_RS12830 Genome accession   NZ_CP092384
Coordinates   2897023..2897430 (+) Length   135 a.a.
NCBI ID   WP_215404157.1    Uniprot ID   -
Organism   Vibrio gigantis strain ACE001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2892023..2902430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MID13_RS12815 (MID13_12815) pdhR 2893951..2894712 (-) 762 WP_009847596.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  MID13_RS12820 (MID13_12820) ampD 2895196..2895795 (-) 600 WP_239716624.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MID13_RS12825 (MID13_12825) nadC 2895879..2896769 (+) 891 WP_239716626.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MID13_RS12830 (MID13_12830) pilA 2897023..2897430 (+) 408 WP_215404157.1 pilin Machinery gene
  MID13_RS12835 (MID13_12835) pilB 2897436..2899121 (+) 1686 WP_239716628.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MID13_RS12840 (MID13_12840) pilC 2899137..2900366 (+) 1230 WP_239716630.1 type II secretion system F family protein Machinery gene
  MID13_RS12845 (MID13_12845) pilD 2900434..2901303 (+) 870 WP_239716632.1 prepilin peptidase Machinery gene
  MID13_RS12850 (MID13_12850) coaE 2901306..2901920 (+) 615 WP_239716634.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 14118.94 Da        Isoelectric Point: 4.5994

>NTDB_id=657090 MID13_RS12830 WP_215404157.1 2897023..2897430(+) (pilA) [Vibrio gigantis strain ACE001]
MNNNKRKNQKGFTLIELMIVVAIIGVLSAIAVPAYKDYVQKSEVSSALATLKSLQTPAEMAFQENGTIASLDELGTASGA
SSLGEITLNPDSSVSASIGFTFDDGALSGKKLQLSRESTGWSCTHDTGITLEGCS

Nucleotide


Download         Length: 408 bp        

>NTDB_id=657090 MID13_RS12830 WP_215404157.1 2897023..2897430(+) (pilA) [Vibrio gigantis strain ACE001]
ATGAATAACAACAAAAGAAAAAATCAGAAAGGTTTTACCTTAATTGAATTGATGATAGTGGTGGCGATCATTGGTGTTCT
ATCTGCTATTGCTGTACCTGCTTACAAAGACTACGTTCAAAAAAGCGAAGTTTCATCCGCACTAGCTACTTTAAAATCCC
TTCAAACACCGGCTGAAATGGCCTTCCAAGAAAATGGTACAATCGCAAGTTTAGATGAATTAGGTACAGCTTCTGGGGCC
AGTTCACTAGGAGAGATTACATTGAATCCAGACTCCAGTGTATCTGCATCAATTGGTTTCACTTTTGATGACGGTGCTCT
TTCTGGGAAAAAACTTCAGTTAAGCCGAGAGAGCACTGGTTGGAGTTGTACTCATGATACAGGTATAACTCTTGAAGGTT
GTTCTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-128)

Prokaryotic N-terminal methylation motif (N_methyl)

(6-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

51.049

100

0.541
  pilA Pseudomonas aeruginosa PAK

42.568

100

0.467
  pilA Vibrio cholerae strain A1552

41.892

100

0.459
  pilA Vibrio cholerae C6706

41.892

100

0.459
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.892

100

0.459
  pilA Acinetobacter baumannii strain A118

38.028

100

0.4
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.6

92.593

0.385
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.778

100

0.378