Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   L8M16_RS04250 Genome accession   NZ_CP091977
Coordinates   890398..891633 (-) Length   411 a.a.
NCBI ID   WP_011238274.1    Uniprot ID   Q5P276
Organism   Aromatoleum aromaticum strain pCyN1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 885398..896633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L8M16_RS04220 - 885566..886009 (-) 444 WP_011238268.1 barstar family protein -
  L8M16_RS04225 - 886006..886371 (-) 366 WP_011238269.1 ribonuclease domain-containing protein -
  L8M16_RS04230 aspS 886589..888388 (-) 1800 WP_011238270.1 aspartate--tRNA ligase -
  L8M16_RS04235 - 888433..889059 (-) 627 WP_011238271.1 DUF502 domain-containing protein -
  L8M16_RS04240 - 889075..889341 (-) 267 WP_011238272.1 FmdB family zinc ribbon protein -
  L8M16_RS04245 pilD 889480..890331 (-) 852 WP_011238273.1 A24 family peptidase Machinery gene
  L8M16_RS04250 pilC 890398..891633 (-) 1236 WP_011238274.1 type II secretion system F family protein Machinery gene
  L8M16_RS04255 pilB 891650..893365 (-) 1716 WP_011238275.1 type IV-A pilus assembly ATPase PilB Machinery gene
  L8M16_RS04260 - 893481..894305 (-) 825 WP_011238276.1 inner membrane protein YpjD -
  L8M16_RS04265 ffh 894348..895706 (+) 1359 WP_011238277.1 signal recognition particle protein -
  L8M16_RS04270 - 895723..896487 (+) 765 WP_011238278.1 EI24 domain-containing protein -

Sequence

Protein


Download         Length: 411 a.a.        Molecular weight: 44167.03 Da        Isoelectric Point: 10.0826

>NTDB_id=654817 L8M16_RS04250 WP_011238274.1 890398..891633(-) (pilC) [Aromatoleum aromaticum strain pCyN1]
MATTTRAGRAVPARSRETLYSWEGKDKNGKLVRGEMRAGGPSLVQATLRRQGVLVSKVRKQKMSRGHKITDKDIALFTRQ
LATMMKSGVPLLQAFDIGIKGSGNPSLARLLNEVRTDVETGSSLSQAFARHPLYFDKLFCNLVAAGEQAGILDGLLDRIA
TYKEKVLAIKGKIKSAMFYPIAVVVVAAIVVTVIMLFVVPEFKKVFTSFGADLPAPTLMVIAISDAFVAYWYILFGIIAA
TVIGIGAMYKRSDAMQIAVDRAVLQLPVVGDIIRKATIARWTRTLATMFAAGVPLVEALDSVGGASGNHVYVTATRQIQT
EVSTGTSLTVAMQNATVFPSMVVQMVSIGEESGQLDSMLGKVADFFEQEVDDAVAGLSTLLEPIIMVFLGVVIGGLVVAM
YLPIFKLGQVV

Nucleotide


Download         Length: 1236 bp        

>NTDB_id=654817 L8M16_RS04250 WP_011238274.1 890398..891633(-) (pilC) [Aromatoleum aromaticum strain pCyN1]
ATGGCGACGACAACGCGAGCCGGACGCGCAGTCCCTGCGCGCTCCAGGGAAACGCTCTACAGCTGGGAAGGCAAGGACAA
GAACGGCAAGCTCGTGCGCGGGGAAATGCGCGCGGGCGGGCCGTCGCTCGTGCAGGCGACACTGCGCCGCCAGGGCGTGC
TGGTGAGCAAGGTCAGAAAGCAGAAAATGTCGCGCGGTCACAAGATCACCGACAAGGACATCGCGCTCTTCACCCGCCAG
CTCGCGACGATGATGAAATCCGGCGTGCCGCTGCTGCAGGCCTTCGACATCGGCATCAAGGGTTCCGGCAATCCGAGCCT
CGCACGGCTGTTGAACGAGGTGCGCACCGACGTCGAGACCGGCTCGAGCCTGTCCCAGGCGTTCGCCAGGCATCCGCTCT
ATTTCGACAAGCTGTTCTGCAATCTCGTCGCTGCCGGCGAGCAGGCGGGAATTCTTGACGGCCTGCTGGACCGCATCGCG
ACCTACAAGGAAAAGGTTCTCGCGATCAAGGGCAAGATCAAGTCGGCGATGTTCTACCCGATCGCGGTCGTCGTCGTCGC
AGCGATCGTTGTCACCGTGATCATGCTGTTCGTCGTGCCCGAGTTCAAGAAAGTGTTCACCAGCTTCGGCGCCGACCTGC
CCGCGCCGACGCTGATGGTCATCGCCATCTCGGACGCCTTCGTCGCGTACTGGTACATCCTCTTCGGCATCATCGCGGCA
ACGGTGATCGGCATCGGTGCGATGTACAAACGCTCGGACGCGATGCAGATCGCCGTCGACCGCGCGGTGCTGCAGCTGCC
GGTGGTCGGCGACATTATCCGCAAGGCGACGATCGCGCGCTGGACACGCACGCTGGCGACGATGTTCGCTGCCGGCGTGC
CGCTCGTCGAAGCGCTCGACTCGGTCGGCGGCGCGTCGGGCAACCACGTCTACGTCACGGCGACGCGCCAGATCCAGACC
GAGGTCAGCACCGGCACCAGCCTCACGGTCGCGATGCAGAATGCGACGGTATTCCCGAGCATGGTCGTGCAGATGGTGTC
GATCGGCGAGGAATCCGGGCAGCTCGACTCGATGCTCGGCAAAGTCGCGGACTTCTTCGAACAGGAAGTCGACGACGCCG
TGGCCGGGCTGTCCACGCTGCTCGAGCCGATCATCATGGTGTTTCTCGGCGTGGTCATCGGTGGTCTCGTCGTCGCGATG
TACCTGCCGATCTTCAAGCTCGGCCAGGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(281-403)

Type II secretion system (T2SS), protein F (T2SSF)

(77-200)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5P276

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

52.13

97.08

0.506
  pilC Legionella pneumophila strain ERS1305867

51.759

96.837

0.501
  pilG Neisseria gonorrhoeae MS11

50.126

96.594

0.484
  pilG Neisseria meningitidis 44/76-A

49.622

96.594

0.479
  pilC Acinetobacter baylyi ADP1

49.246

96.837

0.477
  pilC Acinetobacter baumannii D1279779

48.615

96.594

0.47
  pilC Vibrio cholerae strain A1552

40.488

99.757

0.404
  pilC Vibrio campbellii strain DS40M4

41.058

96.594

0.397
  pilC Thermus thermophilus HB27

39.75

97.324

0.387