Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L8M16_RS04255 Genome accession   NZ_CP091977
Coordinates   891650..893365 (-) Length   571 a.a.
NCBI ID   WP_011238275.1    Uniprot ID   Q5P275
Organism   Aromatoleum aromaticum strain pCyN1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 886650..898365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L8M16_RS04235 - 888433..889059 (-) 627 WP_011238271.1 DUF502 domain-containing protein -
  L8M16_RS04240 - 889075..889341 (-) 267 WP_011238272.1 FmdB family zinc ribbon protein -
  L8M16_RS04245 pilD 889480..890331 (-) 852 WP_011238273.1 A24 family peptidase Machinery gene
  L8M16_RS04250 pilC 890398..891633 (-) 1236 WP_011238274.1 type II secretion system F family protein Machinery gene
  L8M16_RS04255 pilB 891650..893365 (-) 1716 WP_011238275.1 type IV-A pilus assembly ATPase PilB Machinery gene
  L8M16_RS04260 - 893481..894305 (-) 825 WP_011238276.1 inner membrane protein YpjD -
  L8M16_RS04265 ffh 894348..895706 (+) 1359 WP_011238277.1 signal recognition particle protein -
  L8M16_RS04270 - 895723..896487 (+) 765 WP_011238278.1 EI24 domain-containing protein -
  L8M16_RS04275 - 896498..897247 (+) 750 WP_041646293.1 molybdopterin-binding protein -
  L8M16_RS04280 - 897244..898017 (+) 774 WP_011238280.1 M48 family metallopeptidase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62214.38 Da        Isoelectric Point: 5.2141

>NTDB_id=654818 L8M16_RS04255 WP_011238275.1 891650..893365(-) (pilB) [Aromatoleum aromaticum strain pCyN1]
MAANPQAALSGLARAMIQHGRLSETDAMSCTTGAASAPNGFILELAHRKLMTNQAVAWFAAETFGYPLLDISAIDSTSIA
RDAIDRKLMSKHQVVALGKRQNRLTVAAADPSNMRALDEIRFQTGLQVDLVVVEADRLARLVESLAETAEQTLRELTGEE
FDMDLLQQQEGPGDAPSEDTQEVDDAPVVKFIQKVLIDAINEGASDIHFEPYEKYYRIRVRTDGVLREIAQPPLILKEKI
AARIKVISRLDISEKRVPQDGRMKLVLSKNKSIDFRVSSLPTLHGEKIVMRILDASSAMLGVDALGYEPEQKKILLDAIE
RPYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNISTAEDPAEINLSGINQVNVNDKAGLTFAASLRAFLRQDPDIIMVGE
IRDLETAEISIKAAQTGHLVLSTLHTNDAPTTLERLKNMGVAPFNIASSVILITAQRLARRLCTCKQPVDIPIEALLQAG
FPADELDGSWQPYGPVGCERCKGSGYKGRLGIYQVMPITDEIAHIIMTNGNSMDIAAQAQREGVKDLRQSGLLKVRQGVT
SLEEVLASTNE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=654818 L8M16_RS04255 WP_011238275.1 891650..893365(-) (pilB) [Aromatoleum aromaticum strain pCyN1]
ATGGCAGCGAATCCCCAGGCCGCGCTGAGCGGCCTCGCACGAGCGATGATCCAGCACGGCCGGTTGTCCGAAACCGACGC
CATGTCGTGCACGACAGGCGCTGCCAGCGCGCCGAACGGATTCATCCTCGAGCTTGCCCATCGCAAACTGATGACGAACC
AGGCGGTCGCCTGGTTCGCCGCCGAAACGTTCGGCTACCCGCTGCTCGACATCTCGGCTATCGACAGCACGAGCATTGCG
CGCGACGCGATCGACCGCAAGCTGATGAGCAAGCACCAGGTCGTCGCGCTCGGCAAGCGGCAGAACCGGCTGACGGTCGC
TGCCGCCGATCCGTCGAACATGCGCGCGCTCGACGAGATCCGTTTCCAGACCGGTCTCCAGGTGGACCTCGTCGTCGTCG
AAGCGGACCGGCTCGCGCGTCTGGTCGAGTCCCTGGCCGAAACCGCCGAACAGACGCTGCGGGAGCTCACGGGGGAGGAG
TTCGACATGGATCTCCTGCAGCAGCAGGAAGGTCCCGGTGACGCCCCGAGCGAGGACACGCAGGAAGTCGATGACGCGCC
GGTCGTCAAGTTCATCCAGAAAGTGCTGATCGACGCGATCAACGAAGGCGCTTCGGATATCCACTTCGAGCCGTACGAGA
AGTATTACCGCATCCGCGTGCGCACCGACGGCGTGCTGCGCGAAATCGCCCAGCCGCCGCTGATCCTGAAGGAGAAGATC
GCCGCCCGGATCAAGGTCATCTCGCGCCTCGACATCTCGGAAAAGCGCGTCCCCCAGGACGGGCGGATGAAGCTGGTGCT
GTCGAAGAACAAGTCGATCGACTTTCGTGTCTCGTCGCTGCCGACGCTGCATGGCGAAAAGATCGTGATGCGGATTCTCG
ACGCTTCGTCGGCAATGCTCGGCGTCGATGCGCTGGGCTACGAACCGGAACAGAAGAAGATCCTGCTCGACGCGATCGAA
CGGCCCTACGGAATGATCCTCGTGACCGGACCGACCGGTTCCGGCAAGACCGTGTCGCTCTATACCTGCCTGAACCTGCT
CAACAAAGCCGGCGTGAATATCTCCACGGCCGAAGACCCGGCCGAAATCAACTTGTCCGGGATCAACCAGGTCAACGTCA
ACGACAAGGCCGGGCTGACTTTCGCCGCATCGCTGCGGGCCTTTCTGCGCCAGGATCCGGACATCATCATGGTCGGCGAA
ATCCGCGACCTCGAAACCGCCGAGATTTCGATCAAGGCCGCGCAGACCGGCCACCTGGTGCTGTCGACGCTGCATACCAA
CGACGCCCCGACGACGCTCGAGCGCCTGAAGAACATGGGCGTAGCGCCTTTCAACATCGCCTCGTCGGTGATCCTGATCA
CGGCGCAACGGCTGGCGCGGCGCCTGTGCACGTGCAAGCAGCCGGTCGACATCCCGATCGAAGCGCTGCTGCAGGCGGGG
TTTCCCGCCGACGAGCTCGACGGCAGCTGGCAGCCCTATGGCCCGGTCGGCTGCGAGCGCTGCAAGGGCAGCGGCTACAA
GGGGCGGCTCGGGATCTACCAGGTCATGCCGATCACCGACGAGATCGCGCACATCATCATGACCAACGGCAACTCGATGG
ACATCGCTGCGCAGGCCCAGCGCGAAGGCGTCAAGGATCTGCGCCAGTCCGGACTGCTGAAAGTCAGGCAGGGCGTCACA
TCGCTCGAAGAAGTCCTCGCTTCAACCAACGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-150)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5P275

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.398

98.949

0.548
  pilB Acinetobacter baumannii D1279779

54.754

99.475

0.545
  pilB Legionella pneumophila strain ERS1305867

51.93

99.825

0.518
  pilF Neisseria gonorrhoeae MS11

55.238

91.944

0.508
  pilB Vibrio cholerae strain A1552

49.174

95.447

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

48.092

91.769

0.441
  pilB Vibrio campbellii strain DS40M4

48.343

89.842

0.434
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.636

100

0.387
  pilF Thermus thermophilus HB27

40.762

91.944

0.375