Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   L8M16_RS04245 Genome accession   NZ_CP091977
Coordinates   889480..890331 (-) Length   283 a.a.
NCBI ID   WP_011238273.1    Uniprot ID   Q5P277
Organism   Aromatoleum aromaticum strain pCyN1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 884480..895331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L8M16_RS04210 - 884875..885099 (+) 225 WP_011238266.1 cysteine-rich CWC family protein -
  L8M16_RS04215 nudB 885096..885566 (-) 471 WP_011238267.1 dihydroneopterin triphosphate diphosphatase -
  L8M16_RS04220 - 885566..886009 (-) 444 WP_011238268.1 barstar family protein -
  L8M16_RS04225 - 886006..886371 (-) 366 WP_011238269.1 ribonuclease domain-containing protein -
  L8M16_RS04230 aspS 886589..888388 (-) 1800 WP_011238270.1 aspartate--tRNA ligase -
  L8M16_RS04235 - 888433..889059 (-) 627 WP_011238271.1 DUF502 domain-containing protein -
  L8M16_RS04240 - 889075..889341 (-) 267 WP_011238272.1 FmdB family zinc ribbon protein -
  L8M16_RS04245 pilD 889480..890331 (-) 852 WP_011238273.1 A24 family peptidase Machinery gene
  L8M16_RS04250 pilC 890398..891633 (-) 1236 WP_011238274.1 type II secretion system F family protein Machinery gene
  L8M16_RS04255 pilB 891650..893365 (-) 1716 WP_011238275.1 type IV-A pilus assembly ATPase PilB Machinery gene
  L8M16_RS04260 - 893481..894305 (-) 825 WP_011238276.1 inner membrane protein YpjD -

Sequence

Protein


Download         Length: 283 a.a.        Molecular weight: 31098.70 Da        Isoelectric Point: 6.6862

>NTDB_id=654816 L8M16_RS04245 WP_011238273.1 889480..890331(-) (pilD) [Aromatoleum aromaticum strain pCyN1]
MPDLFHDLLLFTALATFVGLFVGSFLNVVIHRLPRMMEREWHAQAAELRGESLPEQETYNLAVPRSRCPHCGHMITAFEN
IPVISYLVLRGRCRHCSAPIGRRYPTVEMLTAVLSGYAAWHFGFGLAAAGAMLFLWTMVALAFIDLDTQLLPDSLTLPLL
WLGLLFNLDGTYSELPQAVVGAIAGYLALWAVYWLFKLATGKEGMGYGDFKLLAAIGAWLGWQMLPLTILFSSLVGAVTG
LGLIVFARHGRDVPIPFGPYLAAAGVLALFWGEPLTQLYLGSL

Nucleotide


Download         Length: 852 bp        

>NTDB_id=654816 L8M16_RS04245 WP_011238273.1 889480..890331(-) (pilD) [Aromatoleum aromaticum strain pCyN1]
ATGCCCGACCTCTTTCACGACCTTCTCCTGTTCACCGCGCTCGCGACGTTCGTCGGCCTTTTCGTCGGCAGCTTCCTGAA
CGTCGTCATCCACCGGTTGCCGCGCATGATGGAGCGCGAATGGCACGCCCAGGCTGCCGAGCTGCGCGGCGAATCGCTCC
CCGAGCAGGAAACCTACAATCTGGCGGTGCCGCGCTCGCGCTGCCCGCACTGCGGCCACATGATCACCGCGTTCGAGAAC
ATCCCGGTCATCAGCTATCTTGTCCTGCGCGGGCGATGCCGTCACTGCAGCGCGCCCATTGGCCGCCGCTACCCGACCGT
CGAAATGCTCACCGCTGTGCTCTCGGGCTACGCTGCGTGGCACTTCGGCTTCGGCCTCGCGGCCGCCGGCGCAATGCTTT
TCCTGTGGACAATGGTCGCGCTCGCGTTCATCGATCTCGACACCCAGCTGCTGCCCGACAGCCTTACCCTCCCGCTGCTG
TGGCTCGGCCTGCTTTTCAATCTCGATGGCACGTACAGCGAGCTCCCGCAGGCCGTCGTCGGCGCGATCGCCGGTTACCT
CGCGCTGTGGGCAGTGTACTGGCTGTTCAAGCTCGCCACCGGCAAGGAAGGCATGGGCTACGGCGATTTCAAGCTGCTCG
CCGCGATCGGGGCGTGGCTCGGATGGCAGATGCTGCCCTTGACGATCCTGTTCTCGTCGCTCGTCGGCGCGGTCACCGGC
CTCGGCCTGATCGTTTTCGCCCGGCACGGCCGGGACGTGCCGATTCCGTTCGGCCCCTATCTCGCCGCTGCCGGCGTGCT
GGCGCTGTTCTGGGGCGAGCCGCTCACGCAGTTGTACCTCGGCAGCCTGTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(132-241)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5P277

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

54.513

97.88

0.534
  pilD Vibrio cholerae strain A1552

54.71

97.527

0.534
  pilD Acinetobacter nosocomialis M2

48.54

96.82

0.47
  pilD Acinetobacter baumannii D1279779

48.54

96.82

0.47
  pilD Neisseria gonorrhoeae MS11

50

89.753

0.449