Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L3V77_RS14345 Genome accession   NZ_CP091975
Coordinates   3146484..3147731 (+) Length   415 a.a.
NCBI ID   WP_275134746.1    Uniprot ID   -
Organism   Vibrio sp. DW001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3141484..3152731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3V77_RS14325 (L3V77_14310) pdhR 3142916..3143683 (-) 768 WP_195704260.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  L3V77_RS14330 (L3V77_14315) ampD 3143968..3144519 (-) 552 WP_275134744.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L3V77_RS14335 (L3V77_14320) nadC 3144706..3145593 (+) 888 WP_275134745.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L3V77_RS14340 (L3V77_14325) - 3146022..3146426 (+) 405 WP_275136785.1 pilin -
  L3V77_RS14345 (L3V77_14330) pilB 3146484..3147731 (+) 1248 WP_275134746.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  L3V77_RS14350 (L3V77_14335) - 3147768..3148991 (+) 1224 WP_275134747.1 type II secretion system F family protein -
  L3V77_RS14355 (L3V77_14340) pilD 3149028..3149897 (+) 870 WP_275134748.1 A24 family peptidase Machinery gene
  L3V77_RS14360 (L3V77_14345) coaE 3149899..3150501 (+) 603 WP_275134749.1 dephospho-CoA kinase -
  L3V77_RS14365 (L3V77_14350) zapD 3150540..3151280 (+) 741 WP_275134750.1 cell division protein ZapD -
  L3V77_RS14370 (L3V77_14355) yacG 3151305..3151502 (+) 198 WP_275136786.1 DNA gyrase inhibitor YacG -
  L3V77_RS14375 (L3V77_14360) rplS 3151623..3151976 (-) 354 WP_195704269.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 415 a.a.        Molecular weight: 46042.07 Da        Isoelectric Point: 6.5010

>NTDB_id=654795 L3V77_RS14345 WP_275134746.1 3146484..3147731(+) (pilB) [Vibrio sp. DW001]
MDIIRNISNDQLSELVPLSNEELNTTSAEIQRNDPISRYINQILLEAMQKRSSDIHFEPYEHKYRIRLRCDGVLVEIDSP
PPQLANRLSSRIKVMANLDIAEKRLPQDGRMKHTLTSKQSVDIRVSTLPTLWGEKIVLRLLKTQSVPLDITALGLSQTQM
DLYVSAISKPQGLVLITGPTGSGKTISLYTALTYLNTPEKNISTAEDPIEINLLGINQVQMNDKIDLNFTSTLKALLRQD
PDIIMIGEIRDIESAEIVIKAAQTGHLVLSTLHTNSASEAVTRLLNMGVDKFSLASCLSLIVAQRLTRALCPACKELDLE
TSSNRKLNPRGSLQIYKANPHGCADCLSGYSGLIGIFELLNIDKTLMRTIERQSSASEINDIALENGFIPLSDSGRNKLI
EGITSIKELQRTLIF

Nucleotide


Download         Length: 1248 bp        

>NTDB_id=654795 L3V77_RS14345 WP_275134746.1 3146484..3147731(+) (pilB) [Vibrio sp. DW001]
ATGGATATAATTCGAAATATCAGCAATGACCAACTATCGGAATTGGTTCCTCTTTCTAATGAAGAACTAAACACAACCAG
TGCTGAGATTCAAAGAAATGACCCTATTAGCCGTTATATCAACCAAATTTTGTTAGAAGCAATGCAGAAAAGGTCCTCAG
ATATTCATTTCGAGCCTTATGAACATAAATACCGTATTCGCTTACGATGTGATGGTGTATTGGTCGAGATAGATTCTCCC
CCACCTCAATTAGCAAATAGGTTGTCCTCTAGAATAAAGGTAATGGCCAACTTAGATATTGCAGAGAAAAGACTTCCGCA
AGACGGACGGATGAAACATACCTTAACGTCTAAACAGAGCGTTGATATCCGCGTCTCCACATTGCCAACATTATGGGGAG
AAAAAATCGTCCTTCGATTACTCAAAACGCAATCAGTCCCATTAGACATAACAGCCCTAGGCTTATCCCAAACGCAAATG
GACCTCTATGTGTCTGCAATTAGTAAACCTCAAGGATTGGTGTTAATCACCGGTCCTACGGGAAGTGGTAAAACCATTTC
ACTATATACCGCGCTCACCTATCTCAATACACCAGAAAAGAACATATCAACAGCTGAAGATCCGATCGAGATTAACCTAT
TAGGTATCAATCAAGTGCAAATGAACGACAAAATCGACCTGAACTTCACCTCCACCTTAAAGGCCCTTCTAAGGCAAGAC
CCTGACATCATTATGATCGGAGAAATTCGTGATATTGAGAGCGCTGAGATTGTAATTAAAGCGGCCCAAACTGGTCACCT
TGTACTTTCCACACTGCATACCAACTCAGCTAGTGAAGCGGTGACTCGACTGCTTAACATGGGGGTAGATAAATTTAGCC
TAGCCTCATGCTTATCATTAATCGTCGCGCAGAGACTTACACGAGCACTATGTCCTGCATGCAAGGAATTAGATTTGGAG
ACCTCTTCGAATCGAAAGCTAAACCCTCGAGGTTCTCTACAAATATACAAAGCTAATCCTCACGGATGCGCCGACTGTCT
CAGCGGCTACTCAGGATTGATTGGTATATTCGAATTGCTAAACATAGACAAAACCCTTATGAGAACAATTGAACGCCAAA
GCAGCGCTTCTGAAATAAACGACATAGCGTTAGAGAATGGTTTTATTCCTCTATCTGATTCAGGGAGAAATAAATTGATT
GAAGGGATAACGAGTATCAAAGAATTGCAACGAACCTTAATCTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(42-309)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

55.422

100

0.554
  pilB Vibrio campbellii strain DS40M4

55.825

99.277

0.554
  pilB Vibrio parahaemolyticus RIMD 2210633

55.583

99.277

0.552
  pilB Acinetobacter baylyi ADP1

52.332

93.012

0.487
  pilB Legionella pneumophila strain ERS1305867

50.505

95.422

0.482
  pilB Acinetobacter baumannii D1279779

52.083

92.53

0.482
  pilF Neisseria gonorrhoeae MS11

50.128

94.217

0.472
  pilB Haemophilus influenzae 86-028NP

45.408

94.458

0.429
  pilB Haemophilus influenzae Rd KW20

45.408

94.458

0.429
  pilF Thermus thermophilus HB27

42.243

100

0.427
  pilB Glaesserella parasuis strain SC1401

43.864

92.289

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.932

92.048

0.395
  pilB/pilB1 Synechocystis sp. PCC 6803

39.798

95.663

0.381
  ctsE Campylobacter jejuni subsp. jejuni 81-176

39.896

93.012

0.371