Detailed information NTG0065402

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EBS_RS01610
• Genome accession: NZ_AP012978
• Coordinates: 334905..336020 (+)
• Length: 371 a.a.
• NCBI ID: WP_043109041.1
• Uniprot ID: -
• Organism: Endosymbiont of unidentified scaly snail isolate Monju
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 329905..341020

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EBS_RS01585 (EBS_0303)	-	330939..331253 (-)	315	WP_043107000.1	DUF167 family protein	-
EBS_RS01590 (EBS_0304)	-	331253..331828 (-)	576	WP_043107003.1	YggT family protein	-
EBS_RS01595 (EBS_0305)	proC	331832..332659 (-)	828	WP_043107005.1	pyrroline-5-carboxylate reductase	-
EBS_RS01600 (EBS_0306)	-	332957..333643 (-)	687	WP_043107006.1	YggS family pyridoxal phosphate-dependent enzyme	-
EBS_RS01605 (EBS_0307)	pilT	333776..334813 (+)	1038	WP_043107007.1	type IV pilus twitching motility protein PilT	Machinery gene
EBS_RS01610 (EBS_0308)	pilU	334905..336020 (+)	1116	WP_043109041.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EBS_RS01615 (EBS_0309)	-	336037..336900 (-)	864	WP_043107008.1	dihydroorotate dehydrogenase electron transfer subunit	-
EBS_RS13370 (EBS_0310)	-	337070..339856 (+)	2787	WP_171816160.1	MMPL family transporter	-

Sequence

Protein

• Length: 371 a.a.
• Molecular weight: 41412.68 Da
• Isoelectric Point: 6.9280

>NTDB_id=65402 EBS_RS01610 WP_043109041.1 334905..336020(+) (pilU) [Endosymbiont of unidentified scaly snail isolate Monju]
MDFNDIIALMVEKKASDLFITADMPPCIKVNGRVMPISKTRLTEQQSRELTYALMSPEQREEFLHTKECNFAFSARGVGR
FRVSAFVQRDAVGMVLRRIETRIPSFDELGLPTVLQDLAMAKRGLVIFVGATGTGKSTSLAAMVGHRNHNSTGHIISIED
PIEFLHKHGGCIITQREVGVDTESYEVALRNTLRQAPDVILIGEIRTREAMEYAITFAETGHLVLTTLHANNANQALERI
IHFFPDDQHAQVLMDLSLNLRGIVAQQLVPRIDGNGRRAIVEILLNTPLASDLIAKGEVSKLKDLMKRSREHGMITFDQA
LYEACVRGEISEKDAMHYADSANEVRLMLKLGDNRPGARSRDQNAMKLVPE

Nucleotide

• Length: 1116 bp

>NTDB_id=65402 EBS_RS01610 WP_043109041.1 334905..336020(+) (pilU) [Endosymbiont of unidentified scaly snail isolate Monju]
ATGGATTTCAACGACATCATCGCCCTGATGGTGGAAAAGAAGGCATCCGACCTGTTCATCACGGCGGACATGCCACCCTG
CATCAAGGTGAACGGGCGGGTGATGCCCATCTCCAAGACGCGTCTGACCGAGCAACAGAGCCGGGAGCTGACCTATGCCC
TGATGAGTCCGGAACAGCGCGAGGAATTCCTGCACACCAAGGAGTGCAACTTCGCCTTCTCGGCGCGCGGGGTCGGCCGT
TTCCGGGTCAGCGCCTTTGTGCAGCGCGATGCGGTCGGTATGGTGCTGCGCCGTATCGAGACGCGTATTCCCTCTTTCGA
CGAGCTGGGTCTTCCCACGGTATTGCAGGACCTGGCGATGGCCAAGCGGGGGCTGGTGATCTTTGTGGGTGCTACCGGCA
CCGGCAAGTCCACCTCGTTGGCCGCGATGGTCGGTCATCGCAACCACAACAGTACCGGGCATATCATCTCCATCGAGGAC
CCGATCGAGTTCCTGCACAAGCACGGGGGCTGCATCATCACCCAGCGCGAAGTCGGCGTGGATACCGAGTCCTACGAGGT
GGCCTTGCGCAACACTCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGTACCCGAGAGGCCATGGAGTATG
CCATCACCTTCGCCGAGACCGGCCACCTGGTGCTGACCACCCTGCATGCCAACAACGCCAACCAGGCGCTCGAGCGCATC
ATCCATTTCTTTCCGGATGACCAGCATGCGCAGGTGCTCATGGACCTGTCGCTGAACCTGCGTGGCATCGTCGCCCAGCA
GTTGGTACCGCGTATCGACGGCAACGGCCGGCGGGCAATCGTGGAGATCCTGCTCAACACGCCGCTGGCCTCCGACCTGA
TCGCCAAGGGCGAGGTGTCCAAGCTCAAGGATCTGATGAAGCGCTCTCGCGAGCACGGCATGATCACCTTCGACCAGGCG
CTCTACGAGGCCTGCGTGCGCGGTGAAATCAGCGAGAAGGACGCCATGCACTATGCCGATTCGGCCAACGAGGTGCGCCT
GATGCTCAAGCTGGGCGACAACCGGCCCGGTGCGCGCTCGCGCGACCAGAACGCCATGAAGCTGGTGCCGGAGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	66.667	96.226	0.642
pilU	Acinetobacter baylyi ADP1	62.117	96.765	0.601
pilU	Vibrio cholerae strain A1552	59.429	94.34	0.561
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.237	86.523	0.383
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.12	90.027	0.361
pilT	Vibrio cholerae strain A1552	40.12	90.027	0.361
pilT	Legionella pneumophila strain Lp02	40	90.296	0.361
pilT	Legionella pneumophila strain ERS1305867	40	90.296	0.361
pilT	Pseudomonas aeruginosa PAK	39.528	91.375	0.361