Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   LVJ77_RS03955 Genome accession   NZ_CP091521
Coordinates   828199..829467 (+) Length   422 a.a.
NCBI ID   WP_027009371.1    Uniprot ID   -
Organism   Conchiformibius kuhniae strain 17694     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 823199..834467
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ77_RS03935 (LVJ77_11145) hisC 823603..824697 (-) 1095 WP_156900807.1 histidinol-phosphate transaminase -
  LVJ77_RS03940 (LVJ77_11140) aroE 824836..825666 (+) 831 WP_027009368.1 shikimate dehydrogenase -
  LVJ77_RS03945 (LVJ77_11135) mtgA 825663..826349 (+) 687 WP_027009369.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  LVJ77_RS03950 (LVJ77_11130) pilF 826484..828163 (+) 1680 WP_027009370.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LVJ77_RS03955 (LVJ77_11125) pilG 828199..829467 (+) 1269 WP_027009371.1 type II secretion system F family protein Machinery gene
  LVJ77_RS03960 (LVJ77_11120) pilD 829467..830381 (+) 915 WP_027009372.1 A24 family peptidase Machinery gene
  LVJ77_RS03965 (LVJ77_11115) coaE 830385..831011 (+) 627 WP_027009373.1 dephospho-CoA kinase -
  LVJ77_RS03970 (LVJ77_11110) zapD 831124..831879 (+) 756 WP_027009374.1 cell division protein ZapD -
  LVJ77_RS03975 (LVJ77_11105) - 831956..832390 (-) 435 WP_027009375.1 hypothetical protein -
  LVJ77_RS03980 (LVJ77_11100) - 832423..833370 (-) 948 WP_245571932.1 YheT family hydrolase -
  LVJ77_RS03985 (LVJ77_11095) - 833376..833855 (-) 480 WP_376986744.1 DUF2750 domain-containing protein -
  LVJ77_RS03990 (LVJ77_11090) yjgA 833921..834445 (-) 525 WP_027009378.1 ribosome biogenesis factor YjgA -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46247.63 Da        Isoelectric Point: 10.0580

>NTDB_id=652035 LVJ77_RS03955 WP_027009371.1 828199..829467(+) (pilG) [Conchiformibius kuhniae strain 17694]
MAARKPVKTVSAKKAAAFNPKQKEKGNRYQFEGRNLQTESIVRGEVVARNEEEARQKLQRRQVKVLQITKMKKTRDKKIT
QADLTVFTRQLSTMMKAGLPLMQAFDIVAKGHSNATMTRMLLSVRADVEQGTALASAFAKHPKYFDKFYCNLVAAGEAGG
VLEALLDKLATYKEKTQAIKKKVKSALTYPISVIVVAIVLVIVMMMFVLPEFKKVYDGLGAELPALTAFMMNISDIFVAY
GWLIILGLVGSVVALVQIHQRSPAFQKKVDAWLLKMPIFGDIVQKATIARWARTTATLFAAGVPLVEALDSVAGASGNIL
YEEATIEIRSKVNQGISLTSAMQAAQIFPNMVVQMASIGEESGALDDMLNKVAEFYEDEVDNAVAMLSSLMEPIIMVVLG
SIIGVILVAMYLPLFNIGNVFG

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=652035 LVJ77_RS03955 WP_027009371.1 828199..829467(+) (pilG) [Conchiformibius kuhniae strain 17694]
ATGGCAGCCAGAAAACCCGTCAAAACCGTTTCGGCAAAAAAAGCCGCCGCCTTCAATCCCAAACAAAAGGAAAAAGGCAA
CCGCTACCAGTTTGAAGGCCGCAACCTGCAAACCGAATCCATCGTGCGCGGCGAAGTGGTCGCCCGCAACGAAGAAGAAG
CGCGTCAGAAACTGCAGCGCCGCCAAGTCAAGGTGCTGCAAATCACCAAGATGAAAAAAACGCGCGACAAAAAAATCACC
CAAGCCGACCTGACTGTGTTTACCCGCCAATTGTCCACCATGATGAAAGCGGGTTTGCCCCTGATGCAGGCATTCGACAT
CGTGGCAAAAGGACACTCCAACGCCACCATGACGCGCATGCTGCTGAGCGTGCGCGCCGACGTGGAGCAAGGCACGGCAC
TGGCAAGCGCGTTTGCCAAACACCCCAAGTATTTCGACAAATTTTACTGCAACTTGGTGGCGGCGGGCGAAGCGGGCGGC
GTGCTGGAAGCCCTGCTCGACAAATTGGCAACCTATAAGGAAAAAACCCAAGCCATCAAGAAAAAAGTCAAAAGCGCGCT
GACCTACCCGATTTCGGTGATTGTGGTTGCCATCGTGCTGGTGATTGTGATGATGATGTTCGTCTTGCCCGAGTTCAAAA
AGGTGTATGACGGCTTGGGCGCGGAACTGCCCGCGCTGACGGCGTTTATGATGAACATTTCCGATATTTTCGTTGCCTAC
GGCTGGCTGATTATCTTGGGCTTGGTCGGCTCGGTGGTGGCATTGGTGCAGATTCACCAACGCTCGCCCGCTTTCCAGAA
AAAGGTCGATGCGTGGCTGCTGAAAATGCCGATTTTCGGCGACATCGTGCAAAAAGCCACGATTGCGCGGTGGGCGCGGA
CAACGGCAACGCTGTTTGCGGCGGGCGTGCCTTTGGTGGAGGCTTTGGATTCGGTGGCGGGCGCATCGGGCAATATTCTG
TATGAAGAAGCCACGATTGAAATCCGCAGCAAGGTCAATCAGGGTATTTCACTGACTTCCGCCATGCAAGCGGCGCAGAT
TTTCCCGAACATGGTGGTGCAGATGGCGTCCATCGGTGAGGAATCGGGCGCGCTGGACGACATGCTGAACAAGGTTGCCG
AGTTTTACGAAGACGAGGTGGACAACGCGGTGGCGATGCTGTCTTCGCTGATGGAGCCGATTATCATGGTGGTGCTGGGC
AGCATCATCGGTGTGATTTTGGTGGCGATGTACCTGCCCCTGTTCAACATCGGTAACGTGTTCGGCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

66.165

94.55

0.626
  pilG Neisseria meningitidis 44/76-A

65.915

94.55

0.623
  pilC Legionella pneumophila strain ERS1305867

48.485

93.839

0.455
  pilC Pseudomonas stutzeri DSM 10701

45.614

94.55

0.431
  pilC Acinetobacter baumannii D1279779

47.135

90.995

0.429
  pilC Acinetobacter baylyi ADP1

46.234

91.232

0.422
  pilC Vibrio cholerae strain A1552

38.903

95.024

0.37
  pilC Vibrio campbellii strain DS40M4

39.196

94.313

0.37
  pilC Thermus thermophilus HB27

37.108

98.341

0.365