Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LVJ77_RS03950 Genome accession   NZ_CP091521
Coordinates   826484..828163 (+) Length   559 a.a.
NCBI ID   WP_027009370.1    Uniprot ID   -
Organism   Conchiformibius kuhniae strain 17694     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 821484..833163
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ77_RS03925 (LVJ77_11155) - 822410..822976 (-) 567 WP_027009365.1 Uma2 family endonuclease -
  LVJ77_RS03930 (LVJ77_11150) hisB 822973..823563 (-) 591 Protein_775 imidazoleglycerol-phosphate dehydratase HisB -
  LVJ77_RS03935 (LVJ77_11145) hisC 823603..824697 (-) 1095 WP_156900807.1 histidinol-phosphate transaminase -
  LVJ77_RS03940 (LVJ77_11140) aroE 824836..825666 (+) 831 WP_027009368.1 shikimate dehydrogenase -
  LVJ77_RS03945 (LVJ77_11135) mtgA 825663..826349 (+) 687 WP_027009369.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  LVJ77_RS03950 (LVJ77_11130) pilF 826484..828163 (+) 1680 WP_027009370.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LVJ77_RS03955 (LVJ77_11125) pilG 828199..829467 (+) 1269 WP_027009371.1 type II secretion system F family protein Machinery gene
  LVJ77_RS03960 (LVJ77_11120) pilD 829467..830381 (+) 915 WP_027009372.1 A24 family peptidase Machinery gene
  LVJ77_RS03965 (LVJ77_11115) coaE 830385..831011 (+) 627 WP_027009373.1 dephospho-CoA kinase -
  LVJ77_RS03970 (LVJ77_11110) zapD 831124..831879 (+) 756 WP_027009374.1 cell division protein ZapD -
  LVJ77_RS03975 (LVJ77_11105) - 831956..832390 (-) 435 WP_027009375.1 hypothetical protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62453.15 Da        Isoelectric Point: 5.3058

>NTDB_id=652034 LVJ77_RS03950 WP_027009370.1 826484..828163(+) (pilF) [Conchiformibius kuhniae strain 17694]
MSIGLFRVLLQQKLVPQEKIEKYQQAAERGTFLKMIFDDKIITPQDLCALLSRMFNYPQFNINAFSRSKMMTDLLDENQM
MAHRCVPLFKRGRKLFMAVSDPTNIQALQKLVFSSGLSIDLVLVRDDHLNSFLEWFGQSSTSILREMGTDSTLQEAQAQT
MIVDGQEEEDGPIARFIQKVLSDALNAGASDIHFEFYEFMARVRFRVDGQLREVVQPPLNLRSQLASRIKVMAKLDISEK
RIPQDGRIQLQFSPDSKAIDFRVNTMPCLFGEKVVMRILNSDAATLNIDQLGFEDFQKDLIMEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTDDVNISTAEDPAEINLPGINQVNVNDKQGLTFEAALKAFLRQDPDIIMIGEIRDLGTADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLARRLCSACKEPVERPPEPALKRAGFTDEDLAGDWTM
YRPVGCEICKGKGYKGRAGLYEVMPMTEKMKAVIMNNGTEVDIANIAYEEGLVDLRRSGLLKVMQGITSLEEVIATTNE

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=652034 LVJ77_RS03950 WP_027009370.1 826484..828163(+) (pilF) [Conchiformibius kuhniae strain 17694]
ATGAGCATAGGTTTATTTCGGGTCTTGCTGCAACAAAAGCTCGTCCCGCAAGAAAAAATCGAAAAATACCAACAAGCCGC
CGAGCGCGGCACCTTCCTGAAAATGATTTTTGACGACAAAATCATCACCCCTCAGGATTTGTGCGCCCTGTTGTCGCGCA
TGTTCAACTACCCGCAATTCAACATCAACGCGTTTTCGCGCAGCAAAATGATGACCGACCTGCTGGACGAAAACCAAATG
ATGGCACACCGTTGCGTGCCACTGTTCAAGCGCGGCAGAAAACTGTTCATGGCGGTTTCCGACCCCACCAACATCCAAGC
CCTGCAAAAACTCGTCTTCTCTAGCGGCTTGTCGATAGACTTGGTTTTGGTGCGCGACGACCACCTGAACAGCTTTCTCG
AATGGTTCGGACAAAGCAGCACCAGCATCCTGCGCGAAATGGGCACCGACAGCACCCTGCAGGAAGCCCAAGCGCAAACC
ATGATTGTGGACGGACAAGAAGAAGAAGACGGGCCCATCGCCCGTTTTATCCAAAAAGTCCTGTCAGACGCACTCAACGC
CGGCGCATCAGACATTCACTTTGAATTTTACGAATTTATGGCACGGGTGCGTTTCCGCGTGGACGGACAACTGCGCGAAG
TGGTTCAGCCCCCGCTCAACCTGCGCAGCCAGCTTGCCTCACGGATTAAAGTGATGGCAAAGCTCGACATTTCCGAAAAA
CGCATTCCGCAGGACGGACGCATCCAGCTCCAATTCAGCCCAGACAGCAAAGCCATCGACTTTCGTGTGAACACCATGCC
CTGCCTGTTTGGCGAAAAAGTGGTGATGCGTATCCTCAACTCCGATGCCGCCACCCTCAACATCGACCAACTGGGCTTTG
AAGACTTCCAAAAAGACCTGATTATGGAAGCGATTCACCGCCCCTACGGCATGGTGCTGGTAACGGGCCCCACAGGCTCG
GGCAAAACCGTCTCGCTCTACACCTGCCTGAACATTCTCAACACCGACGACGTAAACATCTCCACCGCCGAAGACCCCGC
CGAGATTAACCTGCCCGGCATCAATCAGGTAAACGTGAACGACAAGCAGGGCTTGACCTTTGAAGCCGCGCTCAAAGCCT
TCCTGCGTCAAGACCCGGACATCATCATGATTGGTGAGATTCGCGACTTGGGCACGGCGGACATCGCCATCAAAGCCGCG
CAAACGGGCCATATGGTGTTCTCCACCCTGCACACCAACAACGCCCCCGCCACCCTGTCGCGGATGCTGAACATGGGCGT
TGCGCCGTTCAACATCGCCAGCTCGGTCAATCTGATTATGGCGCAGCGTTTGGCACGGCGTTTGTGTTCCGCCTGCAAAG
AACCGGTGGAGCGTCCGCCCGAACCCGCACTCAAACGCGCCGGCTTTACCGATGAAGACTTAGCGGGCGATTGGACGATG
TACCGCCCCGTCGGCTGCGAAATCTGCAAAGGCAAAGGCTACAAAGGCCGTGCCGGCTTGTACGAAGTGATGCCGATGAC
CGAAAAAATGAAAGCCGTCATCATGAACAACGGCACGGAAGTGGACATCGCCAACATCGCCTACGAAGAAGGCTTGGTGG
ATTTGCGCCGCTCCGGCCTGCTCAAAGTGATGCAGGGCATCACCTCGCTGGAAGAAGTCATCGCCACCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

68.214

100

0.683
  pilB Acinetobacter baumannii D1279779

52.669

100

0.53
  pilB Acinetobacter baylyi ADP1

51.327

100

0.519
  pilB Legionella pneumophila strain ERS1305867

48.142

100

0.487
  pilB Vibrio cholerae strain A1552

44.662

100

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

45.936

94.633

0.435
  pilB Vibrio campbellii strain DS40M4

45.736

92.308

0.422
  pilF Thermus thermophilus HB27

36.106

100

0.365