Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LVJ78_RS08270 Genome accession   NZ_CP091507
Coordinates   1820142..1821272 (+) Length   376 a.a.
NCBI ID   WP_132953201.1    Uniprot ID   A0AAE9GVR8
Organism   Uruburuella suis strain 1258/02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1815142..1826272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ78_RS08250 (LVJ78_08250) - 1816061..1817821 (+) 1761 WP_132953211.1 assimilatory sulfite reductase (NADPH) flavoprotein subunit -
  LVJ78_RS08255 (LVJ78_08255) aroD 1818038..1818796 (-) 759 WP_132953207.1 type I 3-dehydroquinate dehydratase -
  LVJ78_RS08260 (LVJ78_08260) - 1818863..1819180 (-) 318 WP_244802564.1 glycine cleavage system H protein -
  LVJ78_RS08265 (LVJ78_08265) - 1819311..1820015 (-) 705 WP_132953203.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  LVJ78_RS08270 (LVJ78_08270) pilU 1820142..1821272 (+) 1131 WP_132953201.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LVJ78_RS08275 (LVJ78_08275) - 1821672..1821989 (+) 318 WP_132953199.1 hypothetical protein -
  LVJ78_RS08280 (LVJ78_08280) ccoS 1821989..1822213 (+) 225 WP_132953197.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  LVJ78_RS08285 (LVJ78_08285) - 1822197..1823513 (+) 1317 WP_132953195.1 MFS transporter -
  LVJ78_RS08290 (LVJ78_08290) - 1823627..1824154 (+) 528 WP_207894280.1 DUF2062 domain-containing protein -
  LVJ78_RS08295 (LVJ78_08295) - 1824151..1824690 (+) 540 WP_207894279.1 type IV secretion protein Rhs -
  LVJ78_RS08300 (LVJ78_08300) - 1825273..1826184 (-) 912 WP_132953193.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42038.16 Da        Isoelectric Point: 6.1123

>NTDB_id=651752 LVJ78_RS08270 WP_132953201.1 1820142..1821272(+) (pilU) [Uruburuella suis strain 1258/02]
MTSSEPQSAKEALFSWLRHLNKYKGSDLFVTTNFPPAMKVDGKIVPITEEPLTAERCMEIAFNIMSPKQIEEFTNTNECN
FAISLPDTSRFRVNAMVQRGATALVLRAITSKIPTFESLNLPPVLKQIALKKRGLVIFVGGTGSGKSTSLASMIDYRNEN
SQDHIITIEDPIEFVHPHKSCIISQREVGVDTDNWFAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERRTQLLTDLSLNLQAFISQRLVPREHGKGRVAAVEVLLNSPLIADLIHKGEVLGIKEIMKRSN
DIGMITFDQALYELYESGQIGYQDAIKNADSANDLRLDIQLRSRRAQNAGPDLELI

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=651752 LVJ78_RS08270 WP_132953201.1 1820142..1821272(+) (pilU) [Uruburuella suis strain 1258/02]
ATGACCTCTTCAGAACCTCAATCTGCCAAAGAAGCGCTCTTCAGCTGGCTGCGCCACCTTAATAAATACAAAGGCTCCGA
TCTTTTCGTTACCACCAATTTCCCGCCTGCCATGAAAGTGGACGGCAAAATCGTGCCCATCACCGAAGAGCCGCTCACCG
CCGAGCGCTGCATGGAAATCGCGTTCAACATCATGAGCCCCAAGCAAATAGAGGAATTCACCAACACCAACGAATGCAAT
TTCGCCATCAGCCTGCCCGACACCAGCCGTTTCCGTGTAAACGCCATGGTGCAGCGCGGCGCCACCGCGCTGGTGCTGCG
CGCCATCACCAGCAAAATCCCCACGTTTGAAAGCCTCAACCTGCCGCCCGTGTTGAAGCAGATTGCCCTCAAAAAGCGCG
GGCTGGTGATTTTTGTCGGCGGCACAGGCTCGGGTAAATCCACCTCGCTCGCCTCGATGATCGACTACCGCAACGAAAAC
AGCCAAGACCACATCATCACCATCGAAGACCCGATTGAATTTGTGCACCCGCACAAAAGCTGCATCATCAGCCAGCGCGA
AGTGGGCGTAGACACCGACAACTGGTTTGCCGCATTGAAAAACACGCTGCGCCAAGCGCCCGATGTGATTTTGATCGGCG
AAATCCGCGACCGCGAAACCATGGATTACGCCATCGCCTTTGCCGAAACCGGCCACTTATGCATGGCCACGCTGCACGCC
AACAGCACCAACCAGGCGCTCGACCGCATCATCAACTTTTTTCCCGAAGAGCGCCGCACCCAGCTGCTCACCGATTTATC
GCTCAACCTGCAAGCCTTTATCTCGCAGCGGCTGGTGCCGCGCGAGCACGGCAAAGGGCGCGTGGCGGCGGTGGAAGTGT
TGCTCAATTCGCCGCTGATTGCCGATTTGATCCACAAAGGCGAAGTACTCGGCATCAAAGAAATCATGAAGCGCTCCAAC
GACATCGGCATGATTACATTCGACCAAGCGCTCTACGAATTATACGAAAGCGGCCAAATCGGCTATCAAGATGCGATTAA
AAATGCCGATTCCGCCAACGATTTGCGCCTCGATATCCAGCTGCGCAGCCGCCGCGCCCAAAATGCCGGGCCGGATTTGG
AATTGATTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(54-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.536

92.287

0.577
  pilU Acinetobacter baylyi ADP1

57.143

91.223

0.521
  pilU Vibrio cholerae strain A1552

53.073

95.213

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.844

88.564

0.388
  pilT Acinetobacter baumannii D1279779

42.901

86.17

0.37
  pilT Acinetobacter baumannii strain A118

42.901

86.17

0.37
  pilT Acinetobacter nosocomialis M2

42.901

86.17

0.37
  pilT Acinetobacter baylyi ADP1

42.073

87.234

0.367
  pilT Legionella pneumophila strain ERS1305867

42.284

86.17

0.364
  pilT Legionella pneumophila strain Lp02

42.284

86.17

0.364