Detailed information NTG0064860

Bioinformatically predicted

Overview

• Name: pilQ
• Type: Machinery gene
• Locus tag: KW3_RS0111285
• Genome accession: NZ_AKGH01000001
• Coordinates: 2480507..2482222 (-)
• Length: 571 a.a.
• NCBI ID: WP_000788426.1
• Uniprot ID: A0A0K9UP29
• Organism: Vibrio cholerae H1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2475507..2487222

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KW3_RS0111265	-	2476250..2477083 (-)	834	WP_000744680.1	Dam family site-specific DNA-(adenine-N6)-methyltransferase	-
KW3_RS0111270	-	2477153..2478628 (-)	1476	WP_000050195.1	ExeA family protein	-
KW3_RS0111275	aroB	2478683..2479768 (-)	1086	WP_000439805.1	3-dehydroquinate synthase	-
KW3_RS0111280	aroK	2479796..2480320 (-)	525	WP_000818616.1	shikimate kinase AroK	-
KW3_RS0111285	pilQ	2480507..2482222 (-)	1716	WP_000788426.1	type IV pilus secretin PilQ	Machinery gene
KW3_RS0111290	pilP	2482248..2482766 (-)	519	WP_000792032.1	pilus assembly protein PilP	Machinery gene
KW3_RS0111295	pilO	2482756..2483346 (-)	591	WP_000157690.1	type 4a pilus biogenesis protein PilO	Machinery gene
KW3_RS0111300	pilN	2483339..2483923 (-)	585	WP_000902745.1	PilN domain-containing protein	Machinery gene
KW3_RS0111305	pilM	2483913..2484929 (-)	1017	WP_000520444.1	type IV pilus assembly protein PilM	Machinery gene

Sequence

Protein

• Length: 571 a.a.
• Molecular weight: 62340.49 Da
• Isoelectric Point: 4.6119

>NTDB_id=64860 KW3_RS0111285 WP_000788426.1 2480507..2482222(-) (pilQ) [Vibrio cholerae H1]
MKNGLKTYVAQTWLTLWVGLALCASSMVFSAESATANQLENIDFRVNKEKAAVLIVELASPSAVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVESVEVFRKEPSTQLVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLEELGVRWGVMSTNGSHSVGGSIESNLWQKGLLADDEFPVDEFLNVNLASTSANASSIAFQVAKLG
SGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide

• Length: 1716 bp

>NTDB_id=64860 KW3_RS0111285 WP_000788426.1 2480507..2482222(-) (pilQ) [Vibrio cholerae H1]
ATGAAAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTGTGGGTTGGTTTGGCACTTTGTGCTTCTTCCAT
GGTGTTTAGTGCTGAATCCGCTACCGCCAACCAGTTGGAAAACATCGACTTTCGGGTCAACAAAGAGAAGGCGGCGGTCT
TAATTGTCGAATTGGCTTCGCCATCGGCGGTCGTGGATGTGCAAAAAGTCCAAGAAGGGTTGAGCATTGAACTCCTGAAA
ACCGATGTGGCGGATGACAAGCTGTATCTGCTTGATGTGAAAGATTTTTCGACCCCTGTTGAGAGCGTGGAAGTATTCCG
TAAAGAGCCAAGCACACAACTGGTTGTGACTGTCGATGGGGAGTTTCAGCACGATTATACCTTAAAAGGAAAATACCTTG
AGGTCGTGATCAGTAAACTCAAAGCCGATGAAAAGCCTAAGCCCAAGAGTGTCTTGGAAAAAGAAGGCAAGCTGATTTCA
ATTAACTTCCAAGATATTCCGGTGCGTAACGTACTGCAGCTGATTGCCGATTATAACGGTTTCAACTTAGTGGTTTCCGA
TTCGGTGGTGGGTAACCTGACTTTACGCTTGGATGGCGTGCCTTGGCAGCAAGTGTTGGACATTATTCTGCAAGTCAAAG
GGCTCGATAAACGGGTCGATGGTAATGTGATTTTGATTGCGCCGAAAGAGGAACTCGACTTGCGCGAGAAACAAGCGCTC
GAAAAAGCCCGTTTAGCAGAAGAGTTGGGCGATTTAAAATCAGAAATCATCAAGATTAACTTTGCCAAAGCGTCTGACAT
TGCGGCGATGATCGGCGGTGAAGGCAACGTTAACATGTTGTCTGAACGAGGTTCGATCAGCATCGACGAACGAACTAACT
CGCTATTGATCCGAGAGCTGCCCGATAATATTGCTGTGATCCGCGAGATCATTGAATCGCTGGATATTCCGGTCAAACAA
GTTCAAATCGAAGCGCGGATTGTCACCGTCAAAGAAGGTAACTTGGAGGAGCTGGGGGTACGCTGGGGCGTGATGTCGAC
CAATGGTAGCCACTCGGTCGGAGGAAGCATAGAGAGTAACTTATGGCAAAAAGGGTTATTGGCGGATGATGAATTTCCGG
TGGATGAATTCTTAAATGTGAATTTGGCTTCCACCTCTGCCAATGCGTCTTCGATTGCTTTCCAAGTCGCTAAATTGGGC
TCAGGTACGTTGTTGGATTTGGAGCTTTCAGCATTACAAAATGAATCGAAGGCCGAAATCATCTCTAGCCCTCGTTTGAT
TACCACTAATAAACAGCCTGCCTATATTGAGCAAGGTACTGAGATCCCTTATCTGGAATCGTCTTCCAGTGGTGCCTCTA
CGGTCGCGTTTAAAAAAGCGGTATTAAGCTTGAAGGTGACACCACAAATCACCCCAGATAACCGCTTGGTGCTCGACTTG
AGTGTGACCCAAGACCGACGCGGTGAAACGGTGAAAACCGGTACGGGTGAAGCGGTATCGATCGATACTCAGCGTATTGG
CACTCAAGTGTTGGTGAATAACGGAGAAACTGTGGTGTTGGGTGGCATTTTCCAACACAGCATCAATAATTCGGTAGATA
AGGTGCCACTGCTGGGGGACTTGCCTGTTTTGGGGGCGTTATTCAGACGAACCTATGAGCAAATGGGCAAAAGTGAACTG
CTGATTTTTGTCACGCCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

• Bacterial type II and III secretion system protein (Secretin) (411-569)

• Bacterial type II/III secretion system short domain (Secretin_N) (255-320)

3D structure

• AlphaFold DB: A0A0K9UP29

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilQ	Vibrio cholerae strain A1552	100	100	1
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	100	100	1
pilQ	Vibrio campbellii strain DS40M4	78.545	96.322	0.757
pilQ	Legionella pneumophila strain ERS1305867	42.778	94.571	0.405
pilQ	Legionella pneumophila strain Lp02	42.778	94.571	0.405
comQ	Acinetobacter baylyi ADP1	39.336	100	0.394
pilQ	Acinetobacter baumannii strain A118	37.819	100	0.389
pilQ	Acinetobacter baumannii D1279779	37.819	100	0.389
pilQ	Pseudomonas aeruginosa PAK	39.344	96.147	0.378